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Molecular Anatomy of RNA Polymerase : Mapping of Transcription Factor Contact Sites

Research Project

Project/Area Number 06454672
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionNational Institute of Genetics

Principal Investigator

ISHIHAMA Akira  National Institute of Genetics, Department of Molecular Genetics, Professor, 分子遺伝研究系, 教授 (80019869)

Co-Investigator(Kenkyū-buntansha) FUJITA Nobuyuki  National Institute of Genetics, Department of Molecular Genetics, Assistant Prof, 分子遺伝研究系, 助手 (90173434)
山岸 正裕  国立遺伝学研究所, 分子遺伝研究系, 助手 (00220252)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsRNA polymerase / Transcription factor / Transcription regulation / Molecular assembly / Protein structure / Transcription signal / Protein-protein communication / 転写調節 / 分子間相互作用 / 分子解剖 / 遺伝解析 / 蛋白-蛋白相互作用 / 蛋白-DNA相互作用 / 構造機能相関
Research Abstract

RNA polymerase with the catalytic activity of RNA synthesis plays a Key role in gene transcription. Swiching of the global pattern of transcription takes place by modulating the promoter selectivity of RNA polymerase after interaction with various trans-acting protein and nucleotide factors. In order to understand the molecular mechaninism of promoter selectivity control of RNA polymerase, we have carried out a systematic mapping of the transcription factor contact sites on RNA polymerase. For this purpose, we reconstituted mutant E.coli RNA polymerases consisting of deletion, insertion or amino acid substitution mutant subunits and examined their responses to various transcription factors. Results indicated that : (i) the molecular contact between RNA polymerase and transcription factors involves narrow regions of about 10 amino acid residues on each component ; (ii) the contact sites are clustered on a short segment of each subunit, i.e., class-I factors on the C-terminal domain of alpha subunit and class-II factors on the C-terminal domain of sigma subunit ; and (iii) both protein factors and DNA enhancer signals interact with the same protein surface of RNA polymerase subunits as analyzed by NMR.The same line of studies has been initiated for mapping of protein-protein contact sites on RNA polymerase II from fission yeast S.pombe and on RNA polymerase from influenza viruses.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (159 results)

All Other

All Publications (159 results)

  • [Publications] Giladi, H.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoters of bacteriophage λ." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imamura, R.: "Identificiation of the cpdA gene encoding cyclic 3′,5′-adenosine monophosphate phosphodiesterase in Escherichia coli." J. Biol. Chem.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K-W.: "Ambidextrous transcription activation by SoxS: Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol. Microbiol.19(2). 307-314 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K-W.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawagishi-Kobayashi, M.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol. Gen. Genet.205(1). 1-5 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.: "Subunit assembly in vitro of Escherichia coli RNA polymerase: Role of the amino-terminal assembly domain of alpha subunit." Genes To Cells. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, M.: "Influenza virus PBI protein is the minimal and essential subunit of RNA polymerase." Arch. Virol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kusano, S.: "Promoter selectivity of Escherichia coli RNA Polymerase Eσ^<70> and Eσ^<38> holoenzymes: Effect of DNA supercoiling." J. Biol. Chem.271(4). 1998-2004 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai, S.: "The cyclic AMP receptor protein(CRP) gene function is required for expression of α(cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect. Immun.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Rajkumari, K.: "Effects of H-NS and potassium glutamate on σ^S-and σ^<70>-directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J. Bacteriol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T.: "Molecular assembly of influenza virus RNA polymerase: Identification of the subunit- subunit contact sites on each P protein subunit." J. Gen. Virol.(in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T.: "Molecular dissection of influenza virus RNA polymerase: PBI subunit alone is able to catalyze RNA synthesis." Virus Genes. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Asano, Y.: "Molecular anatomy of influenza virus RNA polymerase: Photoaffinity labeling of PBI subunit with 8-azido GTP." J. Biochem.117(3). 677-682 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Azuma, Y.: "Isolation of thermolabile RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene." J. Biochem.118(7). 216-220 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Choy, H.: "A. and Adhya, S.: Repression and activation of transcription by Gal and Lac repressors: Involvement of alpha-subunit of RNA polymerase." EMBO J.14(18). 4523-4529 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ding, Q.: "Promoter selectivity of osmoregulated promoters between E^D and E^S holoenzymes." Mol. Microbiol.16. 649-656 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fukuchi, J.: "Decrease in cell viability due to the accumulation of spermidine in spermidine in spermidine acetyltransferease-deficient mutant of Escherichia coli." J. Biol. Chem.270(32). 18831-18835 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishihara, A.: "Genetic strategies of bacteria for stationary-phase survival." Actinomycetologia. 9. 236-243 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K. -W.: "Purification and regulatory properties of MarA protein: a tarnscriptional activator of Escherichia coli multiple antiviotic and superoxide resistance promoters." J. Bacteriol.177. 7100-7104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jeon, Y. H.: "Solution structure of the C-terminal domain of RNA polymerase α subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Science. 270. 1495-1997 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jishage, M.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli: Intracellular levels of σ^<70> and σ^<38>." J. Bacteriol.177. 6832-6835 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, A.: "Gene organization of chicken anemia virus." Virology. 209(1). 480-488 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Insertion analysis of the amino-terminal assembly domain." J. Mol. Biol.248(2). 756-767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Amino acid substitutution within the amino-terminal assembly domain." J. Mol. Biol.254(3). 342-349 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kolb, A.: "Selectivity of the Escherichia coli RNA polymerase Eσ^<38> for overlapping promoters and ability to support CRP activation." Nucleic Acids Res.23(5). 819-826 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumar, A.: "A partially functional 245 amino acid internal deletion derivative of Escherichia coli sigma-70." J. Bacteriol.177(17). 5193-5196 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Lawley. B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J. Bacteriol.177(1). 238-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Liu, X.: "The C-terminal region of the α subunit of Escherichia coli RNA polymerase is required for transcription activation of the flagellar level II operons by the FlhD/FlhC complex." J. Bacteriol.177(17). 5186-5188 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakagawa, Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome, but is involved in capped RNA synthesis." J. Virol.69. 728-733 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Negishi, T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase: Structural domains identified by proteolytic cleavage." J. Mol. Biol.248(2). 723-728 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamagishi, M.: "Isolation of temperature-sensitive mutants of mRNA capping enzyme in Saccharomyces cerevisiae." Mol. Gen. Genet.249. 147-154 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Attey, A.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22(21). 4375-4380 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Busby, S.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein: Receptors bound in tandem at promoters can interact synergistically." J. Mol. Biol.241(1). 341-352 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, N.: "Systematic sequencing of the Escherichia coli genome: Analysis of the 2.4-4.1 min(110,917-193,643)region." Nucleic Acids Res.22(9). 1637-1639 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishihama. A.: "Molecular anatomy of viral RNA-directed RNA polymerase." Arch. Virol.134(1). 235-258 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kajitani, M.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Qβ." J. Bacteriol.176(2). 531-534 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinkawa, H.: "Purification and characterization of RNA polymerase holoenzyme (Eσ^B) from vegetative-phase mycelia of Streptomyce griseus." J. Biochem.118(3). 488-493 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ^<38>(the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tao, K.: "Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase α subunit." J. Bacteriol.177. 6740-6744 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T.: "Role of GTPase activity of Mxl protein in nuclear localization and antiviral activity." J. Gen. Virol.76. 1867-1869 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wada, A.: "Ribosome modulation factor: Stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli." Biochem. Biophys. Res. Commun.214(2). 410-417 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Xuan, X.: "Characterization of pseudorabies virus glycroprotein gII expressed by recombinant baculovirus." Virus Res.36. 151-161 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase: Deletion analysis of the amino-terminal assembly domain." J. Mol. Biol.242(1). 107-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, M.: "Molecular dissection of influenza virus nucleoprotein(NP): Deletion mapping of the RNA-binding domain." J. Virol.68(12). 8433-8436 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumar, A.: "Role of the simga70 subunit of Escherichia coli RNA polymerase in transcription activation." J. Mol. Biol.235(2). 405-413 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai, S.: "DNA sequence analysis of an allelic variant of the Actinomybacillus pleuropneumoniae-RTX-toxin(ApxlA)from serotype 10." Microb. Pathogenesis. 15. 485-495 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sharif, K.A.: "Epitope mapping and functional characterization of monoclonal antibodies for the α subunit of E. coli RNA polymerase." J. Biol. Chem.269. 23655-25660 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi, K.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toriyama, S.: "Nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses." J. Gen. Virol.75. 3569-3579 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T.: "Replication in vitro of the influenza virus genome: Selective dessociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch. Virol.136(3/4). 269-286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuda, J.: "Growth control of influenza A virus by M1 protein: Analysis of transfectant viruses carrying the chimeric M gene." J. Virol.68(12). 8141-8146 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zou, C.: "Asymmetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J. Mol. Biol.236. 1283-1288 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, N.: "Reconstitution of RNA polymerase." Meth. Enzymol., Academic Press, New York(In press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Giladi, H., Murakami, K., Ishihama, A.and Oppenheim, A.B.: "Identification of an UP element within the IHF binding site at the P_L1-P_L2 tandem promoters of bacteriophage lambda." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Imamura, R., Yamanaka, K., Ogura, T., Hiraga, S., Fujita, N., Ishihama, A.and Niki, H.: "Identificiation of the cpdA gene encoding cyclic 3', 5'-adenosine monophosphate phosphodiesterase in Escherichia coli." J.Biol.Chem.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K.-W., Fawcett, W.P., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Ambidextrous transcription activation by SoxS : Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of superoxide inducible genes." Mol.Microbiol.19 (2). 307-314 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K.-W., Yu, X., Skarstad, K., Thony, B., Fujita, N., Ishihama A.and Wolf, Jr., R.E.: "Transcription activation of promoters of the superoxide and multiple antibiotic resistance regulation by Rob, a binding protein of the Escherichia coli origin of chromosomal replication." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kawagishi-Kobayashi, M., Yamamoto, M.and Ishihama, A.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol.Gen.Genet.205 (1). 1-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.and Ishihama, A.: "Subunit assembly in vitro of Escherichia coli RNA polymerase : Role of the amino-terminal assembly domain of alpha subunit" Genes To Cells. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, M., Toyoda, T.and Ishihama, A.: "Influenza virus PB1 protein is the minimal and essential subunit of RNA polymerase." Arch.Virol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kusano, S., Ding, Q., Fujita, N., and Ishihama, A.: "Promoter selectivity of Escherichia coli RNA Polymerase Esigma^<70> and Esigma^<38> holoenzymes : Effect of DNA supercoiling." J.Biol.Chem.271 (4). 1998-2004 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai, S., Nagasawa, Y., Yagihashi, T.and Ishihama, A.: "The cyclic AMP receptor protein (CRP) gene function is required for expression of alpha (cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect.Immun.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Rajkumari, K., Kusano, S., Ishihama, A., T.Mizuno and Gowrishankar, J.: "Effects of H-NS and potassium glutamate on sigma^S and sigma^<70> -directed transcription in vitro from osmotically regulated P1 and P2 promoters of proU in Eschercihia coli." J.Bacteriol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T., Adyshev, D.M., Kobayashi, M., Iwata, A.and Ishihama, A.: "Molecular assembly of influenza virus RNA polymerase : Identification of the subunit-subunit contact sites on each P protein subunit." J.Gen.Virol.(in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T., Kobayashi, M., Nakada, S.and Ishihama, A.: "Molecular dissection of influenza virus RNA polymerase : PB1 subunit alone is able to catalyze RNA synthesis." Virus Genes. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Asano, Y., Mizumoto, K., Maruyama, T.and Ishihama, A.: "Molecular anatomy of influenza virus RNA polymerase : Photoaffinity labeling of PB1 subunit with 8-azido GTP." J.Biochem.117 (3). 677-682 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Azuma, Y., Yasui, K., Yamagishi, M.and Ishihama, A.: "Isolation of thermolabile RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene." J.Biochem.118 (7). 216-220 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Choy, H., Park, S.W., Aki, T., Parrack, P., Fujita, N., Ishihama, A.and Adhya, S.: "Repression and activation of transcription by Gal and Lac Repressors : Involvement alpha-subunit of RNA polymerase." EMBO.14 (18). 4523-4529 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ding, Q., Kusano, S.Villarejo, M.and Ishihama, A.: "Promoter selectivity of osmoregulated promoters between E^D and E^S holoenzymes." Mol.Microbiol.16. 649-656 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fukuchi, J., Kashiwagi, K., Yamagishi, M., Ishihama, A.and Igarashi, K.: "Decrease in cell viability due to the accumulation of spermidine in spermidine in spermidine acetyltransferease-deficient mutant of Escherichia coli." J.Biol.Chem.270 (32). 18831-18835 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishihama, A.: "Genetic strategies of bacteria for stationary-phase survival." Actinomycetologia. 9. 236-243 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jair, K.-W., Martin, R.G., Rosner, J.L., Fujita, N., Ishihama, A.and Wolf, Jr., R.E.: "Purification and regulatory properties of MarA protein : a tarnscriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters." J.Bacteriol.177. 7100-7104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jeon, Y.H., Negishi, T., Shirakawa, M., Yamazaki, M., Fujita, N., Ishihama, A.and Kyogoku, Y.: "Solution structure of the C-terminal domain of RNA polymerase alpha subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Science. 270. 1495-1997 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Jishage, M.and Ishihama, A.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli : Intracellular levels of sigma^<70> and sigma^<38>." J.Bacteriol.177. 6832-6835 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato, A.Fujino, M., Nakamura, T., Ishihama, A.and Otaki, Y.: "Gene organization of chicken anemia virus." Virology. 209 (1). 480-488 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.248 (2). 756-767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Amino acid substitution within the mino-terminal assembly domain." J.Mol.Biol.254 (3). 342-349 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kolb, A., Kotlarz, D., Kusano, S.and Ishihama, A.: "Selectivity of the Escherichia coli RNA polymerase Esigma^<38> for overlapping promoters and ability to support CRP activation." Nucleic Acids Res.23 (5). 819-826 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumar, A., Williamson, H.E., Fujita, N., Ishihama, A.and Hayward, R.S.: "A partially functional 245 amino acid internal delection derivative of Escherichia coli sigma-70." J.Bacteriol.177 (17). 5193-5196 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Lawley, B.Fujita, N., Ishihama, A.and Pittard, A.J.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177 (1). 238-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Liu, X., Fujita, N., Ishihama, A.and Matsumura, P.: "The C-terminal region of the alpha subunit of Escherichia coli RNA polymerase is required for transcription activation of the flagellar level II operons by the FlhD/FlhC complex." J.Bacteriol.177 (17). 5186-5188 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakagawa, Y., Kimura, N., Toyoda, T., Mizumoto, K., Ishihama, A., Oda, K.and Nakada, S.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome, but is involved in capped RNA synthesis." J.Virol.69. 728-733 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Negishi, T., Fujita, N.and Ishihama, A.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase : Structural domains identified by proteolytic cleavage." J.Mol.Biol.248 (2). 723-728 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinkawa, H., Fujita, N., Shiina, T., Tanaka, K., Takahashi, H., Ishihama, A.and Nimi, O.: "Purification and characterization of RNA polymerase holoenzyme (Esigma^B) from vegetative-phase mycelia of Streptomyce griseus." J.Biochem.118 (3). 488-493 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, K., Kusano, S., Fujita, N., Ishihama, A.and Takahashi, H.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing sigma^<38> (the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tao, K.Zou, C.Fujita, N.and Ishihama, A.: "Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase alpha subunit." J.Bacteriol.177. 6740-6744 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T., Asano, Y.and Ishihama, A.: "Role of GTPase activity of Mx1 protein in nuclear localization and antiviral activity." J.Gen.Virol.76. 1867-1869 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wada, A., Igarashi, K., Yoshimura, S., Aimoto, S.and Ishihama, A.: "Ribosome modulation factor : Stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli." Biochem.Biophys.Res.Commun.214 (2). 410-417 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Xuan, X., Nakamura, T., Ihara, T., Sato, I., Tuchiya, K., Nosetto, E., Ishihama, A.and Ueda, S.: "Characterization of pseudorabies virus glycroprotein gII expressed by recombinant baculovirus." Virus Res.36. 151-161 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamagishi, M., Mizumoto, K.and Ishihama, A.: "Isolation of temperature-sensitive mutants of mRNA capping enzyme in Saccharomyces cerevisiae." Mol.Gen.Genet.249. 147-154 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Attey, A., Belyaeva, T., Savery, N., Hoggett, J., Ishihama, A.and Busby, S.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22 (21). 4375-4380 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Busby, S., West, D., Lawes, M., Webster, C., Ishihama, A.and Kolb, A.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein : Receptors bound in tandem at Promoters can interact synergistically." J.Mol.Biol.241 (1). 341-352 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, N., Mori, H., Yura, T.and Ishihama, A.: "Systematic sequencing of the Escherichia coli genome : Analysis of the 2.4-4.1 min (110,917-193,643) region." Nucleic Acids Res.22 (9). 1637-1639 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishihama, A.and Barbier, P.: "Molecular anatomy of viral RNA-directed RNA polymerase." Arch.Virol.134 (1). 235-258 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kajitani, M., Kato, A., Wadda, A., Inokuchi, Y.and Ishihama, A.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Qbeta." J.Bacteriol.176 (2). 531-534 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura, M., Fujita, N.and Ishihama, A.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase : Deletion analysis of the amino-terminal assenbly domain." J.Mol.Biol.242 (1). 107-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kobayashi, M., Toyoda, T., Adyshev, D.M., Azuma, Y.and Ishihama, A.: "Molecular dissection of influenza virus nucleoprotein (NP) : Deletion mapping of the RNA-binding domain." J.Virol.68 (12). 8433-8436 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kumar, A., Grimes, B., Fujita, N., Makino, K., Malloch, R.A., Hayward, R.S.and Ishihama, A.: "Role of the simga70 subunit of Escherichia coli RNA polymerase in transcription activation." J.Mol.Biol.235 (2). 405-413 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nagai, S., Yagihashi, T.and Ishihama, A.: "DNA sequence analysis of an allelic variant of the Actinomybacillus pleuropneumoniae-RTX-toxin (Apx1A) from serotype 10." Microb.Pathogenesis. 15. 485-495 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sharif, K.A., Fujita, N., Igarashi, K., Ishihama, A.and Krakow, J.S.: "Epitope mapping and functional characterization of monoclonal antibodies for the alpha subunit of E.coli RNA polymerase." L.Biol.Chem.269. 23655-23660 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takahashi, K., Hattori, T., Noji, S., Nohno, T., Saito, T., Fujita, N., Ishihama, A.and Taniguchi, S.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toriyama, S., Takahashi, M., Sano, Y., Shimizu, T.and Ishihama, A.: "Nucleotide sequence of RNA 1, the largest genomic segment of rice stripe virus, the prototype of the tenuiviruses." J.Gen.Virol.75. 3569-3579 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyoda, T., Kobayashi, M.and Ishihama, A.: "Replication in vitro of the influenza virus genoma : Selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch.Virol.136 (3/4). 269-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yasuda, J., Bucher, D.J.and Ishihama, A.: "Growth control of influenza A virus by M1 protein : Analysis of transfectant viruses carrying the chimeric M gene." J.Virol.68 (12). 8141-8146 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Zou, C., Fujita, N.and Ishihama, A.: "Asymmetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J.Mol.Biol.236. 1283-1288 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, N.and Ishihama, A.: Reconstitution of RNA polymerase. Meth.Enzymol. (In press). Academic Press, New York.,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Asano,Y.: "Molecular anatomy of influenza virus RNA polymerase:Photoaffinity labeling of PBI subunit with 8-azido GTP.21GC01:J.Biochem." 117. 677-682 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Azuma,Y.: "Isolation of thermolabile RNA polymerase II from fission yeast Schizosaccharomyces pombe with mutations in the subunit 3 gene." J.Biochem.117. 216-220 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Choy,H.: "Repression and activation of transcription by Gal and Lac repressors:Involvement of alpha-subunit of RNA polymerase." EMBOJ.14. 4523-4529 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ding,Q.: "Promoter selectivity of osmoregulated promoters between E^D and E^S holoenzymes." Mol.Microbiol.16. 649-656 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuchi,J.: "Decrease in cell viability due to the accumulation of spermidine in spermidine in spermidine acetyltransferease-deficient mutant of Escherichia coli." J.Biol.Chem.270. 18831-18835 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishihama,A.: "Genetic strategies of bacteria for stationary-phase survival." Actinomycetologia. 9. 236-243 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi,M.: "Influenza virus PBI protein is the minimal and essential subunit of RNA polymerase." Arch.Virol.(1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kolb,A.: "Selectivity of the Escherichia coli RNA polymearase Eσ^<38> for overlapping promoters and ability to support CRP activation." Nucleic Acids Res.23. 819-826 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kumar,A.: "A partially functional 245 amino acid internal deletion derivative of Escherichia coli sigma-70." J.Bacteriol.177. 5193-5196 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kusano,S.: "Promoter selectivity of Escherichia coli RNA Polymerase Eσ^<70> and Eσ^<38> holoenzymes:Effect of DNA supercoiling." J.Biol.Chem.271. 1998-2004 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Lawley,B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177. 238-241 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Liu,X.: "The C-terminal region of the α subunit Escherichia coli RNA polymerase is required for transcription activation of the flagellar level II operons by the FlhD/FlhC complex." J.Bacteriol.177. 5186-5188 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jair,K.-W.: "ambidextrous transcription activation by SoxS:Requirement for the C-terminal domain of the RNA polymerase alpha subunit at a subset of supperoxide-inducible genes." Mol.Microbiol.19. 307-314 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jeon,Y.H.: "Solution structure of the C-terminal domain of RNA polymerase α subunit responsible for the contact with trans-acting transcription factors and cis-acting UP element of promoter." Science. 270. 1495-1997 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jishage,M.: "Regulation of RNA polymerase sigma subunit synthesis in Escherichia coli:Intracellular levels of σ^<70> and σ^<38>." J.Bacteriol.177. 6832-6835 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kawagishi-Kobayashi,M.: "Mutational analysis of the RNase-like domain in subunit 2 of fission yeast RNA polymerase II." Mol.Gen.Genet.205. 1-5 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.248. 756-767 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura,M: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Amino acid substitutution within the amino-terminal assembly domain." J.Mol.Biol.254. 342-349 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nagai,S.: "The cyclic AMP receptor protein(CRP)gene function is required for expression of α (cell-bound)-type hemolysis in an avian pathogenic Escherichia coli strain." Infect.Immun.

    • Related Report
      1995 Annual Research Report
  • [Publications] Nakagawa,Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome,but is involved in capped RNA synthesis." J.Virol.69. 728-733 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Negishi,T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase:Structural domains identified by proteolytic cleavage." J.Mol.Biol.248. 723-728 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinkawa,H.: "Purification and characterization of RNA polymerase holoenzyme(Eσ^B)from vegetative-phase mycelia of Streptomyce griseus." J.Biochem.118. 488-495 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ^<38>(the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tao,K.: "Mapping of the OxyR protein contact site in the C-terminal region of RNA polymerase α subunit." J.Bacteriol.177. 6740-6744 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyoda,T.: "Molecular dissection of influenza virus RNA polymerase:PBI subunit alone is able to catalyze RNA synthesis.21GC25:Virus.Genes"

    • Related Report
      1995 Annual Research Report
  • [Publications] Wada,A.: "Ribosome modulation factor:Stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli." Biochem.Biophys.Res.Commun.214. 410-417 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamagishi,M.: "Isolation of temperature-sensitive mutants of mRNA capping enzyme in Saccharomyces cerevisiae." Mol.Gen.Genet.249. 147-154 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jair,K.-W.: "Purification and regulatory properties of MarA protein:a tarnscriptional activator of Escherichia coli multiple antibiotic and superoxide resistance promoters." J.Bacteriol.177. 7100-7104 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fujita,N.: "Meth.Enzymol." (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Attey,A.: "Interactions between the cyclic AMP receptor protein and the alpha subunit of RNA polymerase at the Escherichia coli galactose operon P1 promoter." Nucleic Acids Res.22. 4375-4380 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Busby,S.: "Transcription activation by the Escherichia coli cyclic AMP receptor protein:Receptors bound in tandem at promoters can interact symergistically." J.Mol.Biol.241. ,341-352 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ding,Q.: "Promoter selectivity control of Escherichia coli RNA polymerase:Differential recognition of osmoregulated promoters between Eo^D and Eo^S holoenzymes." Mol.Microbiol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Deletion analysis of the amino-terminal assembly domain." J.Mol.Biol.242. 107-115 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kimura,M.: "Functional map of the alpha subunit of Escherichia coli RNA polymerase:Insertion analysis of the amino-terminal assembly domain." J.Mol.Biol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kolb,A.: "Selectivity of the E.coli RNA polymerase Eo^<38> for overlapping promoters and ability to suppress CRP activation." Nucleic Acids Res.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kumar,A.: "Role of the simga^<70> subunit of Escherichia coli RNA polymerase in transcription activation." J.Mol.Biol.235. 405-413 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Lawley,B.: "The TyrR protein of Escherichia coli is a class I transcription activator." J.Bacteriol.177. 238-241 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Luo,J.: "Molecular anatomy of the β′subunit of E.coli RNA polymerase:Identification of regions involved in assembly." Biochemistry. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Negishi,T.: "Structural map of the alpha subunit of Escherichia coli RNA polymerase:Structural domains identified by proteolytic cleavage." J.Mol.Biol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Sharif,K.A.: "Epitope mapping and functional characterization of monoclonal antibodies specific for the α subunit of E.coli RNA polymerase." J.Biol.Chem.269. 23655-23660 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahashi,K.: "Repression of in vitro transcription of the Escherichia coli fnr and narX genes by FNR protein." FEBS Lett.340. 59-64 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing o^<38> (the rpoS gene product)." Nucleic Acids Res.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Zou,C.: "Assymetric spacial arrangement of two alpha subunits within the Escherichia coli RNA polymerase." J.Mol.Biol.236. 1283-1288 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kumar,A.: "A functional 245 amino acid internal deletion derivatives of Escherichia coli sigma70." J.Bacteriol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kajitani,M.: "Regulation of the Escherichia coli hfq gene encoding host factor-I for phage Q_β." J.Bacteriol.176. 531-534 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ishihama,A.: "Molecular anatomy of viral RNA-directed RNA polymerases." Arch.Virol.(review). 134. 235-258 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kobayashi,M.: "Molecular anatomy of influenza virus nucleoprotein(NP):Deletion mapping of RNA-binding domain." J.Virol.68. 8433-8436 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nakagawa,Y.: "RNA polymerase PB2 subunit is not required for replication of the influenza virus genome,but is involved in capped RNA synthesis." J.virol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Toriyama,S.: "Nucleotide sequence of RNA 1,the largest genomic segment of rice stripe virus,the prototype of the tenuiviruses." J.Gen.Virol.75. 3569-3579 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toyoda,T.: "Replication in vitro of the influenza genome:Selective dissociation of RNA replicase from virus-infected cell ribonucleoprotein complexes." Arch.Virol.136. 269-286 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,J.: "Growth control of influenza A virus by M1 protein:Analysis of transfectant viruses carrying the chimeric M gene." J.Virol.68. 8141-8146 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Asano,Y.: "Molecular anatomy of influenza virus RNA polymerase:Photoaffinity labelling of PB1 subunit with 8-azido GTP." J.Biochem.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Ishihama,A.: "Molecular Recognitions in Biological Systems" Nova Science Publications,Inc., (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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