| Project/Area Number |
06454673
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Molecular biology
|
|---|
| Research Institution | National Institute of Genetics |
|---|
Principal Investigator |
HIROSE Susumu National Institute of Genetics, Department of Developmental Geneitcs, Professor, 個体遺伝研究系, 教授 (90022730)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
UEDA Hitoshi National Institute of Genetics, Deparment of Developmental Genetics, Research As, 個体遺伝研究系, 助手 (60201349)
|
|---|
| Project Period (FY) |
1994 – 1995
|
| Project Status |
Completed (Fiscal Year 1995)
|
| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥5,700,000 (Direct Cost: ¥5,700,000)
|
|---|
| Keywords | Trnascription / Transcription factor / Drosophila |
|---|
| Research Abstract |
Transcriptional activation of the Drosophila fushi tarazu gene by FTZ-F1 requires two additional factors MBF1 and MBF2. We have purified these mediators from posterior silk glands of the silkworm and cloned cDNA's coding for the factors. MBF1 appears to be a basic protein consisting of 146-amino acid residues and conserved across species from yeast to human. It interacts with FTZ-F1 and increases the FTZ-F1 binding to DNA.This interaction requires the C-terminal half of the FTZ-F1 box within the DNA-binding domain of FTZ-F1. MBF1 also makes a direct contact with TATA element-binding protein TBP.MBF2 is a basic protein consisting of 92-amino acid residues. Native MBF2 bears polysacharides through N-glycoside bonds. MBF2 forms a heterodimer with MBF1 and also binds to beta-subunit of TFIIA.When MBF2 alone was added to transcription mixtures, it activated transcription from various promoters. In contrast, it selectively activated transcriptoin from ftz promoter in the presence of MBF1 and FTZ-F1. Both non-selective and selective activation requires TFIIA.From these results, we have proposed the following model for transcriptional activation by FTZ-F1. MBF1 is a bridging factor that connets between FTZ-F1 and TBP and recruits the positive cofactor MBF2 to a promoter carrying the FTZ-F1-binding site. MBF2 activates transcription in a FTZ-F1-binding site dependent manner through its interaction with the beta-subunit of TFIIA.
|
