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Cyclin-dependent Kinase

Research Project

Project/Area Number 06454676
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionThe University of Tokyo

Principal Investigator

MATSUSHIME Hitoshi  The University of Tokyo, The Institute of Medical Science, Associate Professor, 医科学研究所, 助教授 (50251442)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsCell cycle / D-type cyclins / Cdk4 / MO15 / Cdk inhibitory proteins / Contact inhibition / トランスジェニックマウス / 肝再生 / 抗BrdU抗体 / Cdk4トランスジェニックマウス / モノクロナール抗体 / サイクリンDとCdk4複合体酵素 / p27^<kip1>
Research Abstract

Cyclin-dependent kinase(Cdk)inhibitory proteins are involved in cell cycle arrest induced by antiproliferating factors or chemicals.High cell density also induces cell cycle arrest in which the genomic DNA is unreplicated, even in the presence of a mitotic dose of growth factors ; this is termed contact inhibition.Although the cell cycle of the rat fibroblast cell line, 3Y1, was arrested in quiescence by contact inhibition, the Cdk4 bound to its regulatory subunit, cyclin D1 or D3.However, these complexes were enzymatically inactive.Phosphorylation of the cyclin D1-bound Cdk4 by the Cdk-activating kinase could convert the inactive cyclin D1-Cdk4 complex into its active form in vitro, suggesting that threonine 172 of the Cdk4, of which phosphorylation is required for its activation, was in part unphosphorylated in contact-inhibited 3Y1 cells.Although MO15 was active in cell extracts prepared from the arrested 3Y1 cells, activation of bacterially produced Cdk4 in the cell extracts was inhibited.Removal of p27kip1 from the cell extracts allowed the MO15 holoenzyme to phosphorylate the Cdk4 and in turn activate it, indicating that p27kip1 plays a role in inhibiting the phosphorylation of Cdk4 by MO15 in the contact-inhibited 3Y1 cells.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] 鈴木 健之: "HTLV-1 Tax protein interacts with cyclin-dependent kinase inhibiter p16^<IUK4A> and counteracts its inhibitory activity towards CDK4" EMBO J.15. 1607-1614 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 加藤 章: "Inactivation of the cyclin D-dependent kinase in the rat fibroblast cell line,3Y1,induced by cantact inhibition" J.Biol.Chem.272. 8065-8070 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 加藤 章: "Contact inhibition-induced inactivation of the cyclinD-kapenclent kinase in rat fibroblast cell line,3Y1" Leukemia. 11. 361-362 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki T,et al.: "HTLV-1 Tax protein interacts with cyclin-dependent kinase inhibitor p16INK4A and counteracts its inhibitory activity towards CDK4." EMBO J.15. 1607-1614 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato A,et al.: "Inactivation of the cyclin D-dependent kinase in the rat fibroblast cell line, 3Y1, induced by contact inhibition." J.Biol.Chem.272. 8065-8070 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kato A,et al.: "Contact inhibition-induced inactivation of the cyclin D-dependent kinase in rat fibroblast cell line, 3Y1." Leukemia. 11 Suppl 3. 361-362 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮竹正一郎: "Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas" J.Exp.Med.182. 401-408 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木健之: "HTLV-1 Tax protein interacts with agclin-dependent kinase inhibitor p16^<INK4A> and counteracts its inhibitory activity to CDK4" EMBO J.(発表予定).

    • Related Report
      1995 Annual Research Report
  • [Publications] 松七五三仁: "D-type cyclin-dependent kinase activity in mammalian cells" Mol.Cell.Biol.14. 2066-2076 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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