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Molecular mechanisms and function of apoptosis Faculty of Pharmaceutical Science

Research Project

Project/Area Number 06454679
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionScience University of Tokyo

Principal Investigator

TANUMA Sei-ichi  Science University of Tokyo, Professor, 薬学部, 教授 (10142449)

Co-Investigator(Kenkyū-buntansha) SHIOKAWA Daisuke  Science University of Tokyo, Assistant Professor, 薬学部, 助手 (90277278)
青木 一正  東京理科大学, 薬学部, 助手 (10184029)
内海 文彰  東京理科大学, 薬学部, 助手 (00256679)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywordsapoptpsis / DNA fragmentation / DNA endonuclease / DNase gamma / cell death / ネクローシス / DNA分断化 / ヌクレオソーム / DNaseγ / 胸腺細胞 / プログラム細胞死
Research Abstract

Apoptosis is a mechanism for eliminating unwanted cells from the cell community of multicellular organisms. Abnormalities in the regulation of apoptosis may play a part in the aetiology of cancer, autoimmune diseases, AIDS,degenerative nerve diseases and malformation. On of the hallmarks of apoptosis is the enzymatic cleavage of genomic DNA into nucleosomal oligomers. The identification of an endonuclease responsible for apoptosis might help to explain how this cell suiside is regulated and why DNA is cleaved. Here, we found that gamma type of DNase was retained in apoptptic rat thymocyte nuclei. The mode of DNA cleavage, 3'-hydroxyl (OH) /5'-phosphoryl (P) ends, by homogeneously purified DNase gamma (Mr=33kDa) and its Zn^<2+> sensitivity match those observed in apoptosis in thymocytes induced by irradiation or glucocorticoid treatment, indicating that this endonuclease is a central component of the thymic apoptosis machinery.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Sei-ichi Tanuma: "Molecular mechanisms of apoptosis." Apoptosis in Nomal Development and Cancer (ed.by Mels Sluyser),Taylor & Francis. 39-59 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "Inhibitory effect of tannic acid on human immunodeficiency virus promoter activity induced by 12-0-tetradecanoylphorbol-13-acetate in Jurkat T-cells." Biochem.Biophys.Res.220. 411-417 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "DNA fragmentation during thymic apoptosis is catalyzed by DNaseγ." Apoptosis. 1. 147-152 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "Properties of poly (ADP-ribose) glycohydrolase purified from pig testis nuclei." Arch.Biochem.Biophys.336. 139-146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "Replication factor Crecognizes 5'-phosphate ends of telomeres." Biochem.Biophys.Res.Commun.229. 310-315 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "Restrictive proteolysis of fodrin to 120 kDa fragment is not catalyzed by calpain during thymic apoptosis." Apoptosis. 2. 1-7 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田沼靖一: "アポトーシスとは何か-死からはじまる生の科学-" 講談社, 239 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 田沼靖一: "アポトーシスと創薬" ファルマシア, 140 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Nishikawa, D.Shiokawa, K.Umemori, H.Hayashi, and S.Tanuma: "Occurence of DNase gamma-like apoptptotic endonucleases in hematopoietic cells in Xenopus Laevis and their relation to metamorphosis" Biochem.Biophys.Res.Commun.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Shiokawa, H.Ohyama, T.Yamada, and S.Tanuma: "Purification and properties of DNase gamma from apoptotic rat thymocytes" Biochem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Z.Kouchi, T.C.Saido, H.Ohyama, K.Suzuki, and S.Tanuma: "Restrictive proteolysis of fodrin to 120kDa fragment is not catalyzed by calpain during thymic apoptosis" Apoptosis. 2. 1-7 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Uchiumi, T.Ohta, and S.Tanuma: "Replication factor C recognizes 5'-phosphate ends of telomeres" Biochem.Biophys.Res.Commun.229. 310-315 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Abe, and S.Tanuma: "Properties of poly (ADP-ribose) glycohydrolase purified from pig testis nuclei" Arch.Biochem.Biophys.336. 139-146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Shiokawa, K.Nishimura, H.Maruta, and S.Tanuma: "DNA fragmentation during thymic apoptosis is catalyzed by DNase gamma" Apoptosis. 1. 147-152 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] F.Uchiumi, H.Maruta, J.Inoue, T.Yamamoto, and S.Tanuma: "inhibitory effect of tannic acid on human immunodeficiency virus promoter activity induced by 12-0-tetradecanoylphorbol-13-acetate in Jurkat T-cells." Biochem.Biophys.Res.Commun.220. 411-417 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Tanuma: "In Apoptosis in Normal Development and Cancer (ed.by Mels Sluyser), Taylor & Francis Ltd., London UK" Molecular mechanisms of apoptosis. 39-59 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Tanuma: "DNA fragmentation in apoptosis" Tiss.Cul, Res.Commun.53. 1-9 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Tanuma, and D.Shiokawa: "An endonuclease responsible for apoptosis" In Apoptosis : Prog.Mol.Subcell, Biol.Springer-Verlag. 1-12 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Aoki, H.Maruta, F.Uchiumi, T.Hatano, T.Yoshida, and S.Tanuma: "A Macrocircular ellagitannin, oenothein B,supresses mouse mammary tumor gene expression via inhibition of poly (ADP-ribose) glycohydrolase" Biochem.Biophys.Res.Commun.210. 329-337 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sei-ichi Tanuma: "Molecular mechanisms of apoptosis." Apoptosis in Normal Development and Cancer (ed.by Mels Sluyser). Taylor & Francis. 39-59 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Inhibitory effect of tannic acid on human immunodefiency virus promoter activity induced by 12-0-tetradecanoylphorbol-13-acetate in Jurkat T-cells." Biochem.Biophys.Res.Commun.220. 411-417 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "DNA fragment'ation during thymic apoptosis is catalyzed by DNaseγ." Apoptosis. 1. 147-152 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Properties of poly (ADP-ribose) glycohydrolase purified from pig testis nuclei." Arch.Biochem.Biophys.336. 139-146 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Replication factor C recognizes 5'-phoshate ends of telomeres." Biochem.Biophys.Res.Commun.229. 310-315 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Restrictive proteolysis of fodrin to 120 kDa fragment is not catalyzed calpain during thymic apoptosis." Apoptosis. 2. 1-7 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田沼靖一: "アポトーシスとは何か-死からはじまる生の科学-" 講談社, 239 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田沼靖一: "アポトーシと創薬" ファルマシア, 140 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "A Macrocircular ellagitannin, oenothein B, supresses mouse mammary tumor gene expression via inhibition of poly(ADP-ribose)glycohydrolase." Biochem. Biophys. Res. Commun.210. 329-337 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Induction of autoantibodies in normal mice by injection of nucleobindin and natural occurrence of antibodies against nucleobindin in autoimmune MRL/lpr/lpr mice." Immunol. Lett.45. 35-42 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Apoptosos: a cell ellimination mechanism." Sut. Bull.12. 36-43 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] "Induction of apoptosis by T2 toxin and other natural toxins in HL-60 human promyelotic leukemia cells." Natural Toxins. 3. 129-137 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "DNA fragmentation in apoptosis." Tiss. Cult. Res. Commun.53. 1-9 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sei-ichi Tanuma: "Molecular mechanisms of apoptosis." Prog. Mol. Subcell. Biol.32. 1-12 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田沼靖一: "アポトーシス -細胞の生と死-" 東京大学出版会, 145 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田沼靖一: "アポトーシス -機能と機構-" 日経サイエンス社, 213 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tanuma,S.,et,al.: "Multiple forms of nuclear deoxyribonuclease in rat thymocytes." Biochem.Biophys.Res.Commun.203. 789-797 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shiokawa,D.,et.al.: "Identification of an endonuclease responsible for apoptosis in rat thymocytes." Eur.J.Biochem.226. 23-30 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Aoki,K.,et.al.: "Sodium benzylideneascorbate induce apoptosis in HIV-replicating Vl cells." FEBS Lett.351. 105-108 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Makino.K.,et.al.: "Change in Electrophoretic mobility of HL-60 cells by apoptosis." Colloid and Polimer Sci.272. 487-492 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuribayasi,N.,et.al.: "Introduction of DNA fragmentation in human myelogenous leukemic cell lines by SBA." Anticancer Res.14. 969-976 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kojima,S.,et.al.: "Antioxidative activity of SBA and its adoriamyc in-induced cardiac toxicity." Anticancer Res.14. 1875-1880 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田沼 靖一: "アボトーシス-細胞の生と死-" 東京大学出版会, 164 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田沼 靖一: "アポトーシス実験プロトコール" 秀潤社, 236 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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