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Molecular mechanisms underlying self-sterility in the ascidians

Research Project

Project/Area Number 06454685
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

HOSHI Motonori  Tokyo Inst.Tech.Life Science Professor, 生命理工学部, 教授 (20012411)

Co-Investigator(Kenkyū-buntansha) CHIBA Kazuyoshi  Tokyo Inst.Tech.Life Science Assistant Prof., 生命理工学部, 助手 (70222130)
MATSUMOTO Midori  Tokyo Inst.Tech.Life Science Assistant Prof., 生命理工学部, 助手 (00211574)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥3,600,000 (Direct Cost: ¥3,600,000)
Keywordsallo-recognition / sperm-egg-binding / contact-reaction / hemocyte / glycoprotein / Ascidian / alpha-L-Fucosidase / Metalloendoprotease / アロ認識 / 精子結合 / キメラ胚 / フェノールオキシダーゼ
Research Abstract

Little is so far known on the molecular mechanisms underlying self-sterility in the ascidian gametes, a typical example of the allogeneic recognition. However, an allogeneic interaction named "contact reaction" is well established in the hemocytes of the ascidian, Halocynthia roretzi. On an assumption that allo-recognition molecules in gametes and those in hemocytes are the same or closely related, we searched for such molecules in the gametes and hemocytes of the ascidian. Major findings in this project are itemized as follows.
1) Vacuolate cells secreted phenol oxidase upon contact reaction, which allowed us to develop a quantitative assay for the contact reaction.
2) Vacuolated cells expressed an allo-molecule (s) and its receptor, whereas granular cells did not express such a molecule in a way to be recognized by vacuolated cells.
3) Follicle cells attaching to the vitelline coat expressed an allo-molecule (s).
4) cDNA of sperm alpha-L-fucosidase that mediates sperm binding to the vitelline coat was cloned and partially sequenced.
5) A putative sperm-receptor glycoprotein of 70 kDa was isolated from the vitelline coat and partially sequenced.
6) The gametes became self-fertile in high Mg^<2+>-or caffeine-seawater.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] N.Akita and M.Hoshi: "Hemocytes Relese Phenoloxidase upon Contact Reaction,an Allogeneic Interaction,in the Ascidian Halocynthia roretzi" Cell Struc.Func.20. 81-87 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Hoshi: "In Sixth Lake Shirakaba Placents Conference 1994 Biology of Pregnancy" ed.T.Nakayama and T.Makino,pp.60-65,Keiseisha,Tokyo, 106 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N.Akita and M.Hoshi: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc.Func.20. 81-87 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Hoshi: Allogeneic recognition in ascidian fertilization. In Sixth Lake Shirakaba Placenta Conference 1994 Biology of Pregnancy ed.T.Nakayama and T.Makino, Keiseisha, Tokyo, 60-65 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M. Hoshi: "Allogeneic recognition in ascidian fertilization" Sixth Lake Shirakaba Placenta Conference 1994 Biology of Pregnancy. 60-65 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] N. Akita and M. Hoshi: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc. Func.20. 81-87 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] F. J. Longo, M. Woerner, K. Chiba and M. Hoshi: "Cortical changes in starfish (Asterina pectinifera) oocytes during 1-methyladenine-induced maturation and fertilization/activation" Zygote. 3. 225-239 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] I. Sase, T. Okinaga, M. Hoshi, G. W. Feigenson and K. Kinoshita, J: "Regulatory Mechanisms of the Acrosome Reaction Revealed by Multiview Microscopy of single Starfish Sperm" J. Cell Biol.131. 963-973 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A. Ushiyama, A. Shima and M. Hoshi: "Estimation by radiation inactivation of the minimum functional size of acrosome reaction-inducing substance (ARIS) in the starfish, Asterias amurensis" Zygote. 3(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Chiba and M. Hoshi: "G-protein-mediated signal transduction for meiosis renitiation in starfish oocyte" Progress in Cell Cycle Research. Vol1(in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] F. J. Longo: "Ultrastructural Localization of Acrosome Reaction-Inducing Substance(ARIS) on Sperm of the Starfish, Asterias amurensis" Mol. Rep. Dev. in press. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N. Akita: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc. Func.20. 81-87 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K. Chiba: "A Periodic Network of G Protein βγ Subunit Coexisting with Cytokeratin Filament in Starfish Oocytes" Dev. Biol. in press.

    • Related Report
      1994 Annual Research Report
  • [Publications] M. Hoshi: "Glycosidases, Proteases and Ascidian Fertilization" Seminars Dev. Biol.5. 201-208 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M. Hoshi: "Egg-Jelly Signal Molecules for Triggering the Acrosome Reaction in Starfish Spermatozoa" Internat. J. Dev. Biol.38. 167-174 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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