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Study on the mesenchymal factors regulating gene expression in the epithelium

Research Project

Project/Area Number 06454687
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Developmental biology
Research InstitutionTOKYO METROPOLITAN UNIVERSITY

Principal Investigator

YASUGI Sadao  Faculty of Science, TOKYO METROPOLITAN UNIVERSITY,Professor, 理学部, 教授 (70011591)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
Keywordschicken embryo / digestive tract / epithelial-mesenchymal interactions / transcription factors / induction / gene expression / growth factors / 上皮-間充織相互作用 / 形態形成 / コラーゲン / ペプシノゲン / 消化管上皮 / 消化管間充織 / ペプシノゲン遺伝子 / 組織間相互作用 / 発現調節領域
Research Abstract

We have investigated the molecular nature of mesenchymal factors regulating the gene expression in the epithelilal cells during the development of the digestive tract in the chikcen embryo. First we cleary demonstrated that the differentiation and morphogenesis of the epithelium of the digestive organs are regulated by some factors of the mesenchyme. At the same time the ability to respond to such action of the mesenchyme resided in the epithelium is definitively important. The regional difference of the epithelial responsiveness seemed to be related to the expression patterns of several genes encoding transcriptional factors and sonic hedgehog (morphogen). These genes may define the regional characters. The expreimental embryological methods revealed that the expression of these genes is also under regulation of the mesenchyme. For example, CdxA,which is expressed solely in the posterior gut epithelium is induced in the anterior gut epithelium by the mesenchyme of the intestine. As for the molecular nature of the mesenchymal influences, we postulated that some growth factors are involved in the induction. Especially, bone morphogenetic proteins are very plausible candidates, since the genes encoding these proteins are expressed in the regions which has strong inducing avtivity at the developmental stages relevant to the induction. These results altogether indicate the importance of epithelial-mesenchymal interactions in the organogenesis and give us the valuable information on the molecular mechanisms of the interactions.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (51 results)

All Other

All Publications (51 results)

  • [Publications] Fukuda,K.: "Developmental changes of DNA metlhylation pattcrn of embryonic chicken pepsinogen gene" Journal of Biochemistry. 115. 502-506 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda,K.: "Mesenchymal regulation of epithelial gene eapression inclevecoping avian stomachi 5'-flanking region of papsinogen gene conmediate mesenchymel influence on its expression" Development. 120. 3487-3495 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi,S.: "Regulation of pepsinogen gene expression in epithelial cells of vertebrate stomach during development" International Journal of Developmental Biology. 38. 273-279 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 浦瀬香子: "消化器官の発生における組織間相互作用" 実験医学. 12. 33-40 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 八杉貞雄: "消化器官の分化・形態形成と実験発生学" 遺伝別冊. 6. 117-126 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi,S.: "Differentiation of avian cligestive tract epithelium in vitro" Tissue Culture Research Communication. 14. 177-184 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,D.-Y: "Localization of DNA-synthesizing cells and cell proliferation pattern in developing proventricalar(glanlular,tomach)cp.theliun of emhryonic and hatched chickens" Development,Growth and Differentiation. 38. 1-8 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takiguchi-Hayashi,K.: "Cytokeratin expression in the stomach epithelia of the chicken embryo is regulated by epithelial-mesenchymal interactions" Zoological Science. 13. 263-270 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida,N: "Mucus-associated antigen in epithelial cells of the chicken digestive tract:Developmental chingec in expression and implications for morphogenesis-function relationships" Development,Growth and Differentiation. 38. 185-192 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 福田公子: "消化器官、特に胃の形態形成と分化における成長因子および形態形成因子の役割" 実験医学. 14(8). 1192-1198 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Urase,K.: "Analysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryoinc digestive tract" Roux's Archives of Developmental Biology. 205. 302-390 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 福田公子: "消化管の形態形成と成長因子・転写因子" Molecular Medicine. 33. 796-804 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda,K.: "Aspartic Proteinases" Plenum Press, 629 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi,S.: "Embryos,Endocrine(ells and the Neural Crest)" Witwatersrand University Press, 237 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda, K., Ichinose, M., Saiga, H., Shiokawa, K.and Yasugi, S.: "Developmental changes of DNA methylation pattern of embryonic chick pepsinogen gene." J.Biochem.115. 502-506 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda, K., Ishii, Y., Saiga, H., Shiokawa, K.and Yasugi, S.: "Mesenchymal regulation of epithelial gene expression in developing avian stomach : 5'-flanking region of pepsinogen gene can mediate mesenchymal influence on its expression." Development. 120. 3487-3495 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi, S.: "Regulation of pepsinogen gene expression in epithelial cells of vertebrate stomach during development." Int. J.Dev. Biol.38. 273-279 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Urase, K.and Yasugi, S.: "Tissue interaction in the development of digestive organs.(in Japanese)" Jikkenigaku. 12. 33-40 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi, S.: "Differentiation and morphogenesis of digestive organs, and experimental embryology. (in Japanese)" Iden. 6. 117-126 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi, S.: "Differentiation of avian digestive tract epithelium in vitro." Tiss. Cult. Res. Commun.14. 177-184 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin, D.-Y.and Yasugi, S.: "Localization of DNA-synthesizing cells and cell proliferation pattern in developing proventricular (glandular stomach) epithelium of embryonic and hatched chickens." Dev. Growth & Differ.38. 1-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takiguchi-Hayashi, K., Mizuno, T.and Yasugi, S.: "Cytokeratin expression in the stomach epithelia of the chicken embryo is regulated by epithelial-mesenchymal interactions." Zool. Sci.13. 263-270 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida, N., Urase, K., Takahashi, J., Ishii, Y.and Yasugi, S.: "Mucus-associated antigen in epithelial cells of the chicken digestive tract : Developmental change in expression and implications for the morphogenesis-function relationships." Dev. Growth & Differ.38. 185-192 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda, K.and Yasugi, S.: "The role of growth factors and morphogens in the morphogenesis and differentiation of deigestive tract, especially of stomach. (in Japanese)" Jikkenigaku. 14. 1192-1198 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Urase, K., Fukuda, K., Ishii, Y., Sakamoto, N.and Yasugi, S.: "Analysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryonic digestive tract." Roux's Arch. Dev. Biol.205. 382-390 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda, K.and Yasugi, S.: "Growth factors and transcription factors in the morphogenesis of the gut (in Japanese)" Molecular Medicine. 33. 796-804 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukuda, K., Saiga, H.and Yasugi, S.: Transcription of embryonic chick pepsinogen gene is affected by mesenchymal signals through its 5'-flanking region. Aspartic Proteinases : Structure, Function, Biology, and Biomedical Implications (Ed. Takahashi, K.).Plenum Press, New York, 125-129 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasugi, S.and Mizuno, T.: Mesenchymal influence as a microenvironment regulating the morphogenesis and cytodifferentiation of gut epithelial cells. Embryos, Endocrine Cells and the Neural Crest : A Collection of Essays in Honour of Ann Andrew (Eds. Cramer, B.and Rawdon, B.B.). Witwatersrand University Press, Johannesburg, 99-113 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,D.-Y: "Localization of DNA-synthesizing cells and cell proliferation pattern in developing proventricular (glandular stomaca) spithelium of embrycic and hatched chickens" Development,Growth and Differentiation. 38. 1-8 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takiguchi-Hayashi,K.: "Cytokeratin expression in the stomach epithelia of the chicken embryo is regulated by epithelial-mesenchymal interacticns" Eoological Science. 13. 263-270 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida,N: "Mucus-associated antigen in epithelial cells of the chicken digestive tract : Developmental changes in expression and implications for morphogenesis-fumtion relctionships" Development,Growth and Differentiation. 38. 185-192 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 福田公子: "消化器官、特に胃の形態形成と分化における成長因子および形態形成因子の役割" 実験医学. 14(8). 1192-1198 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Urase,K.: "Aralysis of mesenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryoinc digestive tract" Roux's Archives of Developmental Biology. 205. 382-390 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 福田公子: "消化管の形態形成と成長因子・転写因子" Molecular Medicine. 33. 796-804 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Urase,K.: "Spatial expression of sonic hedge hog in the lung epithelium during branching morphogenesis" Biochemical and Biphysical Research Communications. 225. 161-166 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tabata,H.: "Cultivation of chicken proventricular epithelial cells and their potential for differentiation" Development,Growth and Differentiation. 38. 499-507 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wada,S.: "Spatially and temporally regulated expression of the LIM class homeoboxgene Hrlim suggests multiple distinct functions in development of the ascidian,Halocynthia roretzi" Mechanisms of Development. 51. 115-126 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ysugi,S: "Differentiation of avion digestive tract epithelium in vitro" Tissu Culture Research Communication. 14. 177-184 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katsuyama,Y.: "Expression of the labial group Hox gene HrHox-1 and its alteration induced by retinoil acid in development of the ascidon,Holocynthia roretzi" Development. 121. 3197-3205 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Jin,D.-Y.: "Localization of DNA-synthesizing cells and cell proliferation pattern in developing proventricular (glandular stomach)epithelium of embryonic and hatched chickens" Development,Growth and Differentiation. 38. 1-8 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takiguchi-Hayashi,K.: "Cytokeratin expression in the stomach epithelia of the chiken embryo is regulated by epithelial-mesenchymal interactions" Zoological Science. 13(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Urase,K.: "Analysis of mecenchymal influence on the pepsinogen gene expression in the epithelium of chicken embryonic digestive tract" Roux's Archives of Developmental Biology. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda,K.: "Aspartic Proteinases:Structure,Function,Biology and Biological Imglications" Plenum Press, 629 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda,K.,Ichinose,M.,Saiga,H.,Shiokawa,K.,Yasugi,S: "Developmental changes of DNA methylation pattern of embryoric chick pepsinogen gene" J.Biochem.115. 502-506 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suda,T.et al.: "The role of gravity in chick embryogenesis" FEBS Letters. 340. 34-48 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fukamachi,H.et al.: "Fetal rat glandular stomach epithelial cells differentiate into Surface mucous cells which express cathepsin E in the absence of mesendymal cells in primargcult" Differentiation. 56. 83-89 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasugi,S.: "Regulation of pepsinogen gene expression in epithelial cells of vertebrate Stomach during development" Int.J.Dev,Biol.38. 273-279 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Fukuda,K.,Ishii,Y.,Saiga,H.,Shiokawa,K.,Yasugi,S.,: "Mesenchymal regulation of epithelial gene expression in developing avian stomachis-flanking region of pepsinogeugene can mediate mesenchymal influence onist express" Development. 120. 3487-3495 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八杉 貞雄: "消化器官の分化・形態形成と実験発生学" 遺伝別冊. 6. 117-126 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八杉 貞雄: "生物の世界をさぐる" 岩波書店, 149 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 八杉 貞雄: "生物学を読む" 玉川大学出版部, 185 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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