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Functional morphologic study on NO (nitric oxide) -containing neurons.

Research Project

Project/Area Number 06454692
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionSHIGA UNIVERSITY OF MEDICAL SCIENCE

Principal Investigator

KIMURA Hiroshi  Shiga University of Medical Science, Molecular Neurobiology Research Center, Professor, 分子神経生物学研究センター, 教授 (40079736)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1995: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1994: ¥3,400,000 (Direct Cost: ¥3,400,000)
Keywordsnitric oxide / neuron / development / brain / ganglion / artery / cNOS / iNOS / NO神経 / NO合成酵素 / 構成型NO合成酵素 / 誘導型NO合成酵素 / コリン神経系 / 翼口蓋神経節 / 三叉神経節 / ラット
Research Abstract

Nitric oxide (NO) , agasous radical molecule which affects the environment, has been discovered to be synthesized in certain living cells of vertebrates. This molecule has mutifunctional physiological activities in various organs of the body. The nervous system, in particular, is under the influence of NO with various roles on, for example, neurotransmission, synaptic plasticity, neurotoxicity and neuronal aging. The multiplicity of NO appears to be dynamically regulated by its synthesizing enzyme, nitric oxide-synthase (NOS). In addition to the constitutive NOS (cNOS) which is present in the normal brain, inducible NOS (iNOS) has been known to be expressed in response to injury or inflammation. It is therefore evident that the brain function is associated with the production of NO synthesized by both types of NOS.
In the present study, we studeid the ontogenetic aspect of NO-containing neurons in developing brains of the rat by using immunohistochemical and NADPH-diaphorase histochemical methods. Two prominent NO-containing neuronal cell groups were found, one in the pons and the other in the hypothalamus. As embryos grow, NO-containing neurons were distributed widely throughout various brain regions. The details of such patterns of distribution of No-neurons were mapped at embryonic day 15 and day 19.
The origin and the pattern of projection of NO-containing nerve fibers from the pterygopalatine ganglion to brain arteries were also examined by the NADPH-diaphorase histochemical method combined with axonal tracing methods. The result provided the neuroanatomical basis for differential pharmacologic and physiologic activities of NO in different parts of brain vessels.
In addition, these data were presented in an international symposium organized by the head investigator on July, 1995 at Otsu, as a satellite symposium of the meeting of The International Brain Research Organization.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Taniguchi et al.: "Delayed expression of c-fos protein in rat hippocampus and cerebral cartex after transient exposure to hypoxia." Brain Res.640. 119-125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kinoshita et al.: "Histochemical localization of nitric oxide synthase in rat biliary duats." Biomed. Res.15. 187-193 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Arai T et al.: "Effects of neopterin on ischemic neuronal damage in gerbiles." Neurosci Lett. 173. 107-110 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kimura et al.: "Acidic FGF expression in the surroundings of senile plaques." Tohoku J Exp Med. 174. 279-293 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Minami Y.,et al.: "Projections of nitric oxide synthase-containing fibers from the spheno-palatine ganglion to cerebral arteries in the rat." Neuroscience. 60. 745-759 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Terada H.,et al.: "Distribution of nitric oxide synthase-immunoreactive neurons in fetal rat brains at embryonic day 15 and day 19." J Chem Neuroanat. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Akiyama H,Tooyama I,Kondou H,Ikeda K,Kimura H,EG.Mcgeer, PL.Mcgeer: "Early response of brain resident microglia to kainic acidinduced hippocampal lesions." Brain Res.635. 257-268 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniguchi T,Fukunaga R,Matsuoka Y,Terai K,Kimura H: "Delayd expression of c-fos protein in rat hippocampus and cerebral cortex after transient exposure to hypoxia." Brain Res.640. 119-125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuo A,Tooyama I,Isobe S,Oomura Y,Akiguch I,Hanai K,Kimura J,Kimura H: "Immunohistochemical localization in the rat brain of an epitope corresponding to fibroblast growth factor receptor I." Neuroscience. 60. 49-66 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kinoshita T,Fujimura M,Kimura H: "Histochemical localization of nitric oxide synthase in rat biliary ducts." Biomed.Res.15 (Supplement 2). 187-193 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Minami Y,Kimura H,Aimi Y,SR Vincent: "Projections of nitric oxide synthase-containing fibers from the sphenopalatine ganglion to cerebral arteries in the rat." Neuroscience. 60. 745-759 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Arai T,Watanabe K,Nakao S,Mori H,Mori K,I Tooyama, Kimura H,Kojima S: "Effects of neopterin on ischemic neuronal damage in gerbils." Neurosci.Lett.173. 107-110 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nakao S,Kurata J,Arai T,Murakawa M,Adachi T,Avramov M.N., Mori K,Yasuhara O,Tooyama I,Kimura H: "Lignocaine-induced convulsion does not induce c-fos protein (c-Fos) in rat hippocampus." Acta Anaesth.Scand.38. 845-851 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kitamura Y,Uemura S,Sato T,Yamada N,Morimoto K,Mori A,Kimura H: "Induction of c-fos and resuction of dynorphin in dentate granule cells of a rat model of epilepsy produced by systemic administration of kainic acid : An immunohistochemical study." Psychiatr.Clin.Neurosci.49. S213-S216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsuoka Y,Kitamura Y,Tsukihara T.Terai K,Tooyama I,Kimura H,Taniguchi T: "Neuroprotective effects of NBOX on hypoxia-induced neuronal damage in rat hippocampus." Neuroreport. 6. 2205-2208 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Terada H,Nagai T,Kimura H,Kitahama K,Okada S: "Distribution of nitric oxide synthase-immunoreactive neurons in fetal rat brains at embryonic day 15 and day 19." J.Chem.Neruoanat. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Taniguchi et al.: "Delayed expression of c-fos protein in rat hippocampus and cerebral cortex after transient eposure to hypoxia." Brain Res.640. 119-125 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kinoshita et al.: "Histochemical localization of nitric oxide synthase in rat biliary ducts." Biomed.Res.15. 187-193 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Minami et al.: "Projections of nitric oxide synthase-containing fibers from the sphenopalatine ganglion to cerebral arteries in the rat." Neuroscience. 60. 745-759 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Arai T et al.: "Effects of neopterin on ischemic neuronal damage in gerbiles." Neurosci Lett. 173. 107-110 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kimura et al.: "Acidic FGF expression in the surroundings of senile plaques." Tohoku J Exp Med. 174. 279-293 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Terada et al.: "Distribution of nitric oxide synthase-immunoreactive neurons in rat fetal rat brains at embryonic day 15 and day 19." J Chem Neuroanat. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Akiyama H.: "Early response of brain resident microglia to kainic acid-induced hippocampal lesions." Brain Res.635. 257-268 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Taniguchi T.: "Delayed expression of c-fos protein in rat hippocampus and cerebral cortex after transient exposure to hypoxia." Brain Res.640. 119-125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsuo A.: "Immunohistochemical locaization in the rat brain of an epitope corresponding to fibroblast growth factor receptor I." Neuroscience. 60. 49-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kinoshita T.: "Histochemical localization of nitric oxide synthase in rat biliary ducts." Biomed. Res.(印刷中). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Minami Y.: "Projections of nitric oxide synthase-containing fibers from the sphenopalatine ganglion to cerebral arteries in the rat." Neuroscience. 60. 745-759 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Arai T.: "Effects of neopterin on ischemic neuronal damage in gerbils." Neurosci.Lett.173. 107-110 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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