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Enhancement of non-specific antiviral immunity induced by mucosal administration of immunoadijivant in infant animal

Research Project

Project/Area Number 06454716
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionHokkaido University

Principal Investigator

ARIKAWA Jiro  Hokkaido University School of Medicine, Institute for Animal Experimentation, Professor, 医学部, 教授 (10142704)

Co-Investigator(Kenkyū-buntansha) TANIGUCHI Koki  Sapporo Medical University, Associate Professor, 医学部, 助教授 (40094213)
KARIWA Hiroaki  Graduate School of Veterinary Medicine, Hokkaido University, Lecturer, 大学院・獣医学研究所, 講師 (70224714)
KIDA Hiroshi  Graduate School of Veterinary Medicine, Hokkaido University, Professor, 大学院・獣医学研究所, 教授 (10109506)
AZUMA Ichiro  Institute of Immunological Science, Hokkaido University, Professor, 免疫科学研究所, 教授 (50028411)
橋本 信夫  北海道大学, 獣医学部, 教授 (60082103)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsInfluenzavirus / Rotavirus / Hantavirus / Adjuvant / MDP / Pseudorabies / Mucosal immunity / Animal experiment / インフルエンザウイルス / ウイルス感染
Research Abstract

1. Animal models for studying respiratory infection, enteric infection and systemic infections were established by using mice and Sendai virus, rotavirus and hantavirus, respectively.
2. MDP-Lys (L18) was selected as a immunoadjuvant and examined its effect on the enhancement of protection from infection after its administration by oral, intranasal, intrarectal and subcutaneous routes.
3. Fatal Sendai virus infection was significantly reduced by administration of MDP-Lys (L18) through intranasal, ora and even intra rectal route.
4. Rota virus diarrhea was significantly reduced by administration of MDP-Lys (L18) through subcutaneous, oral and intrarectal route.
5. Fatal hantavirus infection was significantly reduced only by the administration of MDP-Lys (L18) through subcutaneous route.
These results suggested the effect of immunoadjuvant on the augmentation of host immunity by common mucosa immune system. Further studies concerning to the quantitative estimation of the augmentation of host immunity. In addition, enhancement of specific immunity against vaccine antigen by the combination of the immunoadjuvant and mucosal administration should be studied.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Yoshida,R.: "Effect of synthetic lipid A-related compound,DT-5461,on resistance to Sendai virus infection in mice" Immunopharmacology. 28. 153-161 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse,H.: "A potential peptide vaccine against two different strains of influenza virus isolated at intervals of about 10 years." Proc.Natl.Acad.Sci.USA. 91. 9588-9592 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taniguchi,K.: "Differences in plaque size and VP4 sequence found in SA11 virus clones having Simian anthentic VP4" Virology. 198. 325-330 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoo,YC.: "Effect of MDP-Lys (L18),a derivatives of MDP,on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. 13. 1300-1305 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura,M.: "Effect of muramyl dipeptide derivatives as adjuvants ontheinduction of antibody response to recombinant hepatitis B surface antigen." Vaccine. 13. 77-82 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada,A.: "Induction of protective antibody responses against pseudorabies virus by intansal vaccination with glycoprotein B in mice." Archives of Virology. 140. 1629-1635 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kariwa,H.: "Characterization of the mode of Hantaan virus infection in adult mice using a nested reverse transcriptase polymerase chain reaction : transient virus replication in adult mice." Microbiology and lmmunology. 39. 35-41 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada,A.: "Protective immune response of chickens against Newcastle disease, induced by the intranasal vaccination with inactivated virus." Veterinary Microbiology. 50. 17-25 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taniguchi,K.: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides,including a cycteine-rich zinc finger motif-encoding region (nucleotides 156 to 248),or which has a nonsense codon at nucleotides 153 to 155." Journal of Virology. 70. 4125-4130 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukushima,A.: "Effect of MDP-Lys (L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus" Vaccine. 14. 485-491 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuzawa,K.: "Protective effect of mucosal administration of recombinant human macrophage colony-stimulating factor (rhM-CSF) on mucosal infection of Sendai virus in mice." Vaccine. 15. 85-89 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida, R., Sato, K., Yoo, Y.C., Yoshimatsu, K., M.Tamura, M., Ishihara, C., Arikawa, J.and Azuma, I.: "Effect of synthetic lipid A-related compund, DT-5461, on resistance to Sendai virus infection in mice." Immunopharmacology. 28. 153-161 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taniguchi, K., Nishikawa, K., Kobayashi, N., Urasawa, T., Wu, H., Gorziglia, M.and Urasawa, S.: "Differences in plaque size and VP4 sequence found in SA11 virus clones having simian authentic VP4." Virology. 198. 325-330 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Naruse, H., Ogasawara, K., Kaneda, R., Hatakeyama, S., Itoh, T., Hiroshi, K., Miyazaki, T., Good, R.A., Onoe, K.: "A potential peptide vaccine against two different strains of influenza virus isolated at intervals of about 10 years." Proc.Natl.Acad.Sci.USA. 91. 9588-9592 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoo, YC,Yoshimatsu, K,Hatsuse R,Yoshida R,Tonooka S.Arikawa J,Azuma I: "Effect of MDP-Lys (L18), a derivatives of MDP,on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. 13. 1300-1305 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura, M., Yoo, Y.C., Yoshimatsu, K., Yoshida, R., Oka, T., Ohkuma, K,Arikawa, J.and Azuma, I.: "Effect of muramyl dipeptide derivatives as adjuvants ontheinduction of antibody response to recombinant hepatitis B surface antigen." Vaccine. 13. 77-82 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, A.and Kida, H.: "Induction of protective antibody responses against pseudorabies virus by intranasal vaccination with glycoprotein B in mice." Archives of Virology. 140. 1629-1635 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kariwa, H., Kamimura, M., Arikawa, J., Yoshimatsu, K., Takashima, I.and Hashimoto, N.: "Characterization of the mode of Hantaan virus infection in adults mice using a nested reverse transcriptase polymerase chain reaction : transient virus replication in adult mice." Microbiology and Immunology. 39 (1). 35-41 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, A.and Kida, H.: "Protective immune response of chickens against Newcastle disease, induced by the intranasal vaccination with inactivated virus." Veterinary Microbiology. 50. 17-25 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukushima A., Yoo YC,Yoshimatsu K., Matsuzawa K., Tamura M., Tono-oka S., Taniguchi K., Urasawa S., Arikawa J., Azuma I.: "Effect of MDP-Lys (L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and Rotavirus." Vaccine. 14. 485-491 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taniguchi, K., Kojima, K., Urasawa, S.: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cystein-rich zinc finger motif-encoding region (nucleotide 156 to 248) or which has a nonsense codon at nucleotides 153 to 155." Journal of Virology. 70. 4125-4130

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuzawa, K., Yoo, YC., Fukushima, A., Yoshimatsu, K., Arikawa, J.and I.Azuma, I.: "Protective effect of mucosal administration of recombinant human macrophage colony-stimulating factor (rhM-CSF) on mucosal infection of Sendai virus in mice." Vaccine. 15. 85-89 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida, R.: "Effect of synthetic lipid A-related compound, DT-5461, on resistance to Sendai virus infection in mice" Immunopharmacology. 28. 153-161 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Naruse, H.: "A potential peptide vaccine against two different strains of influenza virus isolated at intervals of about 10 years." Proc. Natl. Acad. Sci. USA. 91. 9588-9592 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taniguchi, K.: "Differences in plaque size and VP4 sequence found in SA11 virus clones having Simian anthentic VP4" Virology. 198. 325-330 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoo, YC.: "Effect of MDP-Lys (L18), a derivatives of MDP, on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. 13. 1300-1305 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamura, M.: "Effect of muramyl dipeptide derivatives as adjuvants ontheinduction of antibody response to recombinant hepatitis B surface antigen." Vaccine. 13. 77-82 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takada, A.: "Induction of protective antibody responses against pseudorabies virus by intransal vaccination with glycoprotein B in mice." Archives of Virology. 140. 1629-1635 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kariwa, H.: "Characterization of the mode of Hantaanirus infection in adult mice using a nested reverse transcriptase polymerase chain reaction : transient virus replication in adult mice." Microbiology and Immunology. 39. 35-41 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takada, A.: "Protective immune response of chickens against Newcastle disease, induced by the intranasal vaccination with inactivated virus." Veterinary Microbiology. 50. 17-25 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taniguchi, K.: "Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cycteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153 to 155." Journal of Virology. 70. 4125-4130 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukushima, A.: "Effect of MDP-Lys (L18) as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus" Vaccine. 14. 485-491 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuzawa, K.: "Protective effect of mucosal administration of recombinant human macrophage colony-stimulating factor (rhM-CSF) on mucosal infection of Sendai virus in mice." Vaccine. 15. 85-89 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida,R.: "Effect of synthetic lipid A-related compound,DT-5461,on resistance to Sendai virus infection in mice" Immunopharmacology. 28. 153-161 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yoo,YC.: "Effect of MDP-Lys(L18),a derivatives of MDP,on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. 13. 1300-1305 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamura,M.: "Effect of muramyl dipeptide derivatives as adjuvants ontheinduction of antibody response to recombinant hepatitis B surface antigen." Vaccine. 13. 77-82 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukushima,A.: "Effect of MDP-Lys(L18)as a mucosal immunoadjuvant on protection of mucosal infections by Sendai virus and rotavirus" Vaccine. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Mizuho Tamura: "Effect of muramyl dipeptide derivatives as adjuvants on the induction of antibody response to recombinant hepatitis B surface antigen" Vaccine. 13. 77-82 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yung Choon Yoo: "Effect of MDP-Lys(L18),a derivative of MDP,on enhancing host resistance against Hantaan virus infection in newborn mice." Vaccine. (inpress). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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