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Development of X-ray fluorescence analysis of light elements in biological samples --- application to the study of Alzheimer disease

Research Project

Project/Area Number 06554034
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section試験
Research Field 分離・精製・検出法
Research InstitutionScience University of Tokyo

Principal Investigator

NAKAI Izumi  Science University of Tokyo, Faculty of Science, Department of Applied Chemistry, Professor, 理学部, 教授 (90155648)

Co-Investigator(Kenkyū-buntansha) OGATA Kiyoshi  Hitachi, Ltd. Material Process Department, Production Engineering Research Laboratory, Senior Researcher, 生産技術研究所, 主任研究員
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1996: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1995: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsAluminum / synchrotron radiation / X-ray fluorescence analysis / Alzheimer disease / Total reflection X-ray fluorescence analysis / biological trace element / trace element analysis / biopsy sample / アルツハイマー / 臨床試料 / 蛍光X線 / 微量元素 / 生体試料
Research Abstract

We have constructed a new X-ray fluorescence chamber for total reflection analysis and two-dimensional X-ray fluoresce analysis. This instrument is suitable for trace element analysis of biological samples such as biopsy samples (TR-XRF) and cancer tissues with complex structure (XRF imaging). X-rays from Synchrotron radiation and conventional X-ray tube can be used as the X-ray source. We have used this instrument to determine Al level in biological samples as given below. We have also determined the Zen and Cu levels from biopsy samples.
Synchrotron radiation Induced total reflection X-ray fluorescence analysis of Al in biological samples was developed through examinations of sample preparation technique, internal standard and measurement conditions. The samples were standard solutions of Al and organs of Al administrated rat. The sample was heated in a current of oxygen gas and decomposed with concentrated nitric acid and hydrogen peroxide. Cr was chosen as an internal standard after consideration of its energy, coexisting elements in the sample and simplicity of the sample preparation. It was possible to determine Al of 10ng range using 7.25keV X-ray in air.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] 太田典明: "放射光全反射蛍光X線分析による生体試料中の微量アルミニウムの定量"X線分析の進歩. 28. 221-234 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 近藤奈穂子: "放射光全反射蛍光X線分析による生検試料中のCu,Znの定量"分析化学. 46. 957-964 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Homma: "Site-specific distribution of copper,selenium,and zinc in human kidney by SR-XRF"Nucl. Inst. and Meth.. B103. 229-232 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Homma-Takeda: "Direct detection of mercury-bound metalloproteins(metallo-thionein and Cu-Zn Superoxide dismutase)using a combination of gel electrophoresis and SR-XRF"Anal.Lett.. 29. 601-611 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Numako: "Characterization of kidney granules in marine bivalves by XAFS analysis"Bulletin de I'Insti* oceanograhique、Monaco. 14. 105-113 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Numako: "XAFS studies of iron in the teeth of chiton, Acanthopleura Japonica"J.de Physique IV. 615-616 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Ohta: "Determination of trace amounts of Al in biological samples by Synchrotron radiation-induced total-reflection X-ray fluorescence analysis (in Japanese with English Abstract)"28. 207-216 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N. Konndo: "Synchrotron radiation -induced total- reflection X-ray fluorescence analysis of Cu and Zn in carcinoma tissues obtained by a biopsy (in Japanese with English Abstract)"Bunseki Kagaku. 46. 957-964 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C. Numako: "XAFS studies of iron in the teeth of chiton, Acanthopleura Japonica."J. de Physique. IV C2. 615-616 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C. Numako: "Characterization of biominerals by fluorescence X-ray absorption fine structure (XAFS) technique (in Japanese with English Abstract)"Adv. In X-ray chemical analysis, Japan. 27. 221-234 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Homma-Takeda: "Direct detection of mercury-bound metalloproteins (metallo-thionein and Cu, Zn-Superoxide dismutase) using a combination of gel electrophoresis and one dimensional synchrotron radiation X-ray fluorescence analysis."Anal. Lett.. 29. 601-611 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Homma-Takeda: "A new method for detection of mercury-bound protein with a combination of gel electrophoresis and one dimensional synchrotron radiation X-ray fluorescence analysis."J. Occup. Health.. 38. 118-119 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Homma: "Site-specific distribution of copper, selenium, and zinc in human kidney by synchrotron radiation induced X-ray fluorescence"Nucl. Instr. And Meth. B. 103. 229-232 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C. Numako: "Characterization of kidney granules in marine bivalves by XAFS analysis"Bulletin de l'Institut oceanographique, Monaco. 14. 105-113 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 太田典明: "放射光全反射蛍光X線分析による生体試料中の微量アルミニウムの定量法の開発" X線分析の進歩. 28. 207-216 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 佐々木 明: "Cisplatin投与後のマウス組織内の白金濃度測定" 癌と化学療法. 23. 639-641 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Homma-Takeda: "Direct detection of murcury-bound metallaproteins (metallothionein and Cu,Zn-Superoxide dismutase) using a combination of gel electrophoresis and one deimens synchrotron radiation X-ray fluorescence analysis" Anal.Lett.29・4. 601-611 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Homma-Takeda,S: "A new method for detection of mercury-bound protein with a combination of gel electrophoresis and one dimensional synchrotron radiation X-ray fluorescence analysis" J.Occup Health. 38. 118-119 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S. Homma: "Site-specific distribution of copper, Selenium, and Zinc in human kidney by synchrotron radiation induced X-ray fluorescence" Nacl. Instr. and Meth, in Phys. Res. B. 103. 229-232 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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