| Project/Area Number |
06555237
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
反応・分離工学
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| Research Institution | Osaka University |
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Principal Investigator |
KOMASAWA Isao Osaka University, Dept.of Chemical Engineering, Professor, 基礎工学部, 教授 (40029476)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOMORI Koichiro Miyazaki University Dept.of Material Engineering, Research Fellow, 工学部, 助手 (80235506)
TSUCHIDO Tetsuaki kansai University, Dept.of Bioengineering, Associate Professor, 工学部, 助教授 (50029295)
KUBOI Ryoichi Osaka University, Dept.of Chemical Engineering, Associate Professor, 基礎工学部, 助教授 (40029567)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Aqueous two-phase / Heat shock protein / Bioprocess / E.coli / GroEL / GroES / CAB / Structure formation |
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| Research Abstract |
The effective refolding process was designed and proposed as the extractive refolding process using aqueous two-phase systems (ATPS) mediated with heat shock proteins (HSP) at higher temperature.
In this integrated extractive refolding process which combined and exploited both functions of heat shock proteins and ATPS,the refolding rate can be accelerated suggesting the advantage of refolding at high temperature. Furthermore the activity obtained from more hydrophilic phase can be improved due to prevention of aggregation and to protection from heat inactivation of GroEL and refolded protein can be concentrated into the hydrophilic phase separating from the denatured protein.
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