| Project/Area Number |
06556001
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Breeding science
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| Research Institution | Iwate University |
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Principal Investigator |
KAIZUMA Norihiko Iwate University, President, 教授 (00003773)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Keisuke Agric.Res.Center.Min.Agric.Forest.& Fish., Lavoratory Chief, 農業研究センター, 室長
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Artificial Mutation Induction / Soybean Seed Proteins / Lipoxigenase / Edible Oil / 人為突然変異 / プロテイン・ボディ / スフェロゾーム |
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| Research Abstract |
The ultimate objective of this study is to develope completely lipoxigenase-lacking soybean lines in seeds which are characterized with oil deterioration-resistance by effect of no or less oxidation of non-suturated fatty acids.
Some promising soybean F3 lines were selected from the hybridization between Kyushu 11 (lypoxigenase-lacking) and high oil Jilin 11/Williams in the Agricutural Research Center. However, oil analyzes and oil-deterioration tests are now in progress.
On the other hand, the soybean lines accumulating some seed protein-subunit lacking variant genes (7S and 11S globulin-subunits) through hybridizations were developed in Iwate University for future hybridization programs with lipoxigenade-lacking lines. SDS-PAGE analyzes using about 3,500 F2 seeds were carried out for genetic analyzes of the protein-subunit lacking traits. A part of them were used for oil and protein contents analyzes and very interesting results were obtained.
Electron microscopic observations of the protein bodies and spherosomes in the 7S and/or 11S globulin subunit-lacking variants brought some interesting results.
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