| Project/Area Number |
06556016
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
SHIMIZU Sakaya Kyoto Univ., Fac.of Agr.Prof., 農学部, 教授 (70093250)
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| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Michihiko Kyoto Univ.Fac.of Agr.Assist., 農学部, 助手 (90252494)
KOBAYASHI Michihiko Kyoto Univ.Fac.of Agr.Lec., 農学部, 講師 (70221976)
AKIMOTO Kengo Suntory Ltd.Researcher, 生物医学研究所, 研究員
BABA Naomichi Okayama Univ., Fac.of Agr.Prof., 農学部, 教授 (50027075)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Arachidonic acid / Mead acid / Polyunsaturated fatty acid / Functional lipid / Single cell oil / Mortierella alpina / シングルセルオイル / ジホモ-γ-リノレン酸 / 機能性油脂 / Single Cell Oil / Moritierella alpina |
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| Research Abstract |
Mycelia of a fungus Mortierella alpina 1S-4 and the mutants derived from it are new and rich sources of C_<20> polyunsaturated fatty acids (PUFAs) of pharmacologically and dietary importance. M.alpina 1S-4 produecs a unique triacylglycerol with high n-6 PUFA content. The mycelial content of arachidonic acia reaches 274 mg/g dry mycelia (4.3g/L) on cultivation of the fungus in a medium containing glucose and yeast extract. The value accounts nearly 70% of the total fatty acids in the extracted oil. Mutants defective in DELTA5 desaturase are potent produces of dihomo-gamma-linolenic acid (4.1g/L). ADELTA12 desaturase-defective-mutant produces an oil containing only n-9 PUFAs. The mycelial Mead acid content is 141mg/g dry mycelia (1.9g/L). An oil rich in n-3 PUFAs can be produced by the same mutant when grown in a medium containing linseed oil. The content of total n-3 PUFAs (i.e., alpha-linolenic acid+8,11,14,17-cis-icosatetraenoic acid+5,8,11,14,17-cis-icosapentaenoic acid) is nearly 50% in total mycelial fatty acid. The enzyme systems for the biosynthesis of PUFAs in the Mortierella fungi is also discussed.
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