• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of clonal preadipose cell line from bovine adipose tissue

Research Project

Project/Area Number 06556051
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YANO Hideo  Fac.of Agric., Kyoto University Professor, 農学部, 教授 (20026587)

Co-Investigator(Kenkyū-buntansha) TORII Shin-ichiro  Fac.of Agric., Kyoto University Instructor, 農学部, 助手 (50263132)
KAWADA Teruo  Fac.of Agric., Kyoto University Associate professor, 農学部, 助教授 (10177701)
MATSUI Tohru  Fac.of Agric., Kyoto University Associate professor, 農学部, 助教授 (40181680)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥12,700,000 (Direct Cost: ¥12,700,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1994: ¥8,600,000 (Direct Cost: ¥8,600,000)
KeywordsCattle / Preadipocyte / Adipocyte
Research Abstract

The information on the proliferation and differentiation of preadipocyte would be useful for the control of fat deposition in beef cattle. We have tried in the present study to establish a clonal cell line of preadipocyte from bovine adipose tissue. In addition, adipogenic or antiadipogenic effect of some nutrients or synthetic compounds on bovine preadipocytes were investigated using primary culture system.
Several adipose tissues from calves and intramuscular adipose tissues from adult cattle were used as sources of cells to be cloned because such immature tissues would retain many undifferentiated preadipose cells. Differential trypsinization method and differential cell adhesion method were adopted for selective recovery of preadipocytes. However, we could not find any available clones which maintain preadipose phenotype for generations.
In primary culture, the presence of fetal calf serum in culture medium prevented adipose conversion of bovine preadipocytes. Vitamin C accelerated adipocyte differentiation in serum-free condition. A thiazolidinedione, which is an artificial ligand for murine peroxisome proliferator-activated receptor (PPAR) gamma, powerfully induced adipocyte differentiation of bovine preadipocytes in primary culture. Retinoids blocked this effect. These results suggest the presence of functional PPAR and retinoid receptor in bovine preadipocyte. In addition, these in vitro observations imply that vitamin A usually suppresses adipocyte differentiation in adipose tissue of beef cattle in vivo and that low vitamin A status may favor adipocyte differentiation and thus the development of beef marbling. However, such nutritional stress on commercial beef cattle would not be recommended for economical and ethical reasons. More reliable and acceptable methods for producing highly marbled beef are to be established, which could be achieved through the clarification of the mechanism of adipocyte differentiation in bovine muscle tissue.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 鳥居伸一郎: "ビタミンA,CおよびDがヒツジ由来培養脂肪前駆細胞のグリセロール-3ーリン酸デヒドロゲ-ゼ活性に及ぼす影響" 日本畜産学会報. 66. 1039-1042 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鳥居伸一郎: "肥育牛由来の初代培養脂肪前駆細胞の脂肪細胞への分化" 肉用牛研究会報. 59. 25-29 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鳥居伸一郎: "黒毛和種から単離した脂肪前駆細胞の脂肪細胞への分化におけるビタミンおよび脂肪酸の影響" 肉用牛研究会報. 60. 27-28 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 松井徹: "ビタミンAとウシの脂肪細胞分化" 動物遺伝研究会誌. 24. 37-44 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, K.: "Vitamin A inhibits and fatty and promotes the differentiation of bovine preadipocyte" Proceedings of the 8th AAAP Animal Science Congress. 2. 642-643 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鳥居伸一郎: "ウシの脂肪細胞分化における核内レセブターPPARγとRARの相互作用:リガンドを用いた検討" 日本畜産学会関西支部報. 134. 20-20 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii S,Matsumoto K,Matsui T and Yano H: "Effect of vitamin A,C and D on glycerol-3-phosphate dehydrogenase activity of sheep preadipocytes in primary culure" Animal Science and Technology (in Japanese with English abstract). 66. 1039-1042 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii S,Matsuda K,Matsui T and Yano H: "Primary culture of preadipocytes from beef cattle and its differentiation into adipocytes" Bulletin of Beef Cattle Science (in Japanese with English abstract). 59. 25-29 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii S,Matsuda K,Oh-yama M,Matsui T and Yano H: "Effects of vitamins and fatty acids on the differentiation of preadipocytes isolated from Wagyu beef cattle" Bulletin of Beef Cattle Science (in Japanese). 60. 27-28 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsui T,Torii S and Yano H: "Vitamin A and adipocyte differentiation in beef cattle" The journal of Animal Genetics (in Japanese). 24. 37-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda K,Oh-yama M,Torii S,Matsui T and Yano H: "Vitamin A inhibits and fatty acid promotes the differentiation of bovine preadipocyte" Proceedings of the 8th AAAP Animal Science Congress. 2. 642-643 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Torii S,Matsuda K,Oh-yama M,Matsui T,Kawada T,Oka A and Yano H: "The interaction between PPARgamma and RAR on the differentiation of bovine preadipocyte : in vitro study using specific ligands" The Kansai Journal of Animal Science and Technology (in Japanese). 134. 20 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 松井徹: "ビタミンAとウシの脂肪細胞分化" 動物遺伝研究会誌. 24. 37-44 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Matsuda: "Vitamin A lnhibits and fatty acid promotes the differentiation of bovine preadipocyte." Proceedings of the 8th AAAP Animal Science Congress. 2. 642-643 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鳥居伸一郎: "ウシの脂肪細胞分化における核内レセプターPPARγとRARの相互作用:リガンドを用いた検討" 日本畜産学会関西支部報. 134. 20-20 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鳥居伸一郎・松本和典・松井徹・矢野秀雄: "ビタミンA, CおよびDがヒツジ由来培養脂肪前駆細胞のグリセロール-3ーリン酸デヒドロゲナーゼ活性に及ぼす影響" 日本畜産学会報. 66. 1039-1042 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松田恭子・鳥居伸一郎・松井徹・矢野秀雄: "ウシ脂肪組織由来脂肪前駆細胞の脂肪細胞への分化にトリアシルグリセロールおよび脂肪酸が及ぼす影響" 日本畜産学会関西支部報. 131. 23- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鳥居伸一郎・松田恭子・松井徹・矢野秀雄: "肥育牛由来の初代培養脂肪前駆細胞の脂肪細胞への分化" 肉用牛研究会報. 59. 25-29 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鳥居伸一郎・松田恭子・大山路世・松井徹・矢野秀雄: "黒毛和種から単離した脂肪前駆細胞の脂肪細胞への分化におけるビタミンおよび脂肪酸の影響" 肉用牛研究会報. 60. 27-28 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松本和典・鳥居伸一郎・松井徹・矢野秀雄: "ビタミンCがめん羊の脂肪前駆細胞の分化に及ぼす影響" 日本畜産学会関西支部報. 128. 19 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi