| Project/Area Number |
06556057
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
MORI Tsuneatsu THE UNIVERSITY OF TOKYO,Inst. Medical Science Associate Professor, 医科学研究所, 助教授 (40012760)
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| Co-Investigator(Kenkyū-buntansha) |
SUDOH Tadashi Parmaseutical Res. Lab. Kirin Brewery Co., Ltd. Vice President, 副所長
BABA Tadashi Univ. Tsukuba, Inst. Applied Biochem, Associate Professor, 応用生物系, 助教授 (40165056)
TAKASAKI Seiichi THE UNIVERSITY OF TOKYO,Inst. Medical Science Associate Professor, 医科学研究所, 助教授 (80112093)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | sp38 / sperm / zona pellucida / contraception / 避妊ワクチン / 精子蛋白 / Sp38 / 卵子透明帯糖鎖 / 硫酸化糖鎖 / 透明帯結合蛋白質 |
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| Research Abstract |
We founs a novel sperm protein sp38 with zona pellucida (ZP)-binding activity located at the anterior portion of many mammalian species. It was originally purified from boar epididymal sperm as a 38 kDa glycoprotein. The cDNA clones encoding sp38 was isolated from boar testis cDNA library based on the results of partial amino acid sequences of purified protein. The cDNA encoded a 350-residue amino acid precursor protein which was posttranslationally modified into a mature protein of 299 amino acid residues. Northern blot analysis demonstrated that the transcript of sp38 was expressed only in the testis. Mouse and human homologues of boar sp38 was obtained from testis sDNA libraries of respective species and showed highly homologous sequences. Boar sp38 bound to 90kDa-ZP glycoprotein species in a calcium-dependent manner. The binding characteristics of sp38 to the ZP were similar to those of proacrosin. Antibodies against sp38 inhibited the binding of sperm to the ZP in vitro egg-sperm binding assay.
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