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Studies on Two-Dimensional Imaging of Oxygen Release from Microvessels.

Research Project

Project/Area Number 06557002
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section試験
Research Field General physiology
Research InstitutionEhime University

Principal Investigator

MAEDA Nobuji  Ehime University School of Medicine, Department of Physiology, Professor, 医学部, 教授 (50036464)

Co-Investigator(Kenkyū-buntansha) TATEISHI Norihiko  Ehime University School of Medicine, Department of Physiology, Assistant Profess, 医学部, 助手 (90236555)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsOxygen transport / Microcirculation / Erythrocytes / Two-dimensional imaging / Absorption spectrum / Marginal plasma layr / Fahraeus effect / Image computation / ヘモグロビン / 2次元イメージング / コンピュータ画像 / 画像処理 / 酸素運搬
Research Abstract

An apparatus for the determination of two-dimensional images of oxygen saturation and content of flowing erythrocytes in microvascular network was constructed. (1) The apparatus was composed of an inverted microscope, a video-camera, six interference filters with different wavelength, an image processor and a computer. Based on the difference of absorption spectra of oxygenated and deoxygenated hemoglobin in erythrocytes, the two-dimensional images of oxygen saturation and content of flowing erythrocytes in microvessels were prepared, and the profiles in a crossection of microvessels was also prepared. (2) The quantitative determination using the present apparatus was possible in the range of (hematocrit in %) x (inner diameter in mm) <4.5, thus for all microvessels releasing oxygen to tissues. The accuracy of the measurement was <plus-minus>10%. (3) The decrease of oxygen saturation of erythrocytes in the flow process from arterioles to venules in a microvacular network was imaged. The axial accumulation of erythrocytes, thus the formation of cell-free layr along vessel wall, the Fahraeus effect in narrower microvessels and the inverse Fahraeus effect in capillaires were simultaneously imaged. (4) With the decrease of flow velocity of erythrocytes in microvessels and in acidic pH in the tissues, the oxygen release from flowing erythrocytes was accelerated. (5) The relationship between the rheological properties of erythrocytes (hematocrit, erythrocyte deformation and erythrocyte aggregation) and the flow dynamics of erythrocytes evaluated by the thickness of cell-free layr and the flow resistance was quantitatively evaluated in relation with vascular elasticity. (6) Based on the analysis of reflection spectrum, the oxygen saturation of erythrocytes in microvessels on the surface of brain was also imaged. (7) Several problems for further studies were propose

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Tateishi,N.et al.: "Flow dynamics of erythrocytes in microvessels of isolated rabbit mesentery : Cell-free layer and flow resistance." J.Biomechanics. 27. 1119-1125 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateishi,N.et al.: "Spectrophotometric analysis of oxygen release at single microvessel level." Progress in Microcirculartion Research. 231-234 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Soutani,M.et al.: "Quantitative evaluation of flow dynamics of erythrocytes in microvessels : Influence of erythrocyte aggregation." Am.J.Physiol.268. H1959-H1965 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maeda,N.et al.: "Erythrocyte flow and elasticity of microvessels,evaluated by marginal cell-free layer and flow resistance." Am.J.Physiol.271. H2454-H2461 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateishi,N.et al.: "Oxygen saturation and erythrocyte concentration plane imaging of flowing erythrocytes in microvascular network." 6th World Congress for Microcirculation. 213-216 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maeda,N.: "Erythrocyte rheology in microcirculation." Jpn.J.Physiol.46. 1-14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateishi, N., Suzuki, Y., Soutani, M.and Maeda, N.: "Flow dynamics of erythrocytes in microvessels of isolated rabbit mesentery : Cell-free layr and flow resistance" J.Biomechanics. 27. 1119-1125 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateishi, N., Maeda, N.and Shiga, T.: "Spectrophotometric analysis of oxygen release at single microvessel level" Progress in Microcirculation Research (H.Niimi, M.Oda, T.Sawada, R-J.Xiu, eds), pp.231-234, Pergamon, Tokyo. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Soutani, M., Suzuki, Y., Tateishi, N.and Maeda, N.: "Quantitative evaluation of flow dynamics of erythrocytes in microvessels : Influence of erythrocyte aggregation" Am.J.Physiol. 268. H1959-H1965 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maeda, N., Suzuki, Y., Tanaka, J.and Tateishi, N.: "Erythrocyte flow and elasticity of microvessels, evaluated by marginal cell-free layr and flow resistance" Am.J.Physiol. 271. H2454-H2461 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tateishi, N., Suzuki, Y.and Maeda, N.: "Oxygen saturation and erythrocyte concentration plane imaging of flowing erythrocytes in microvascular network" 6th World Congress for Microcirculation (K.Messmer, W.M.Kubler, eds), pp.213-216, Monduzzi Editore, Bologna. (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Maeda, N.: "Erythrocyte rheology in microcirculation" Jpn.J.Physiol. 46. 1-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,Y.,Tateishi,N.,Soutani,M.et al.: "Deformation of erythrocytes in microvessels and glass capillaries : Effect of erythrocyte deformability." Microcirculation. 3. 49-57 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,Y.,Tateishi,N.,Soutani,M.et al.: "Flow behavior of erythrocytes in microvessels and glass capillaries : Effect of erythrocyte deformation and...." Int.J.Microcirc.16. 187-194 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda,N.,Suzuki,Y.,Tanaka,J.et al.: "Erythrocyte flow and elasticity of microvessels,evaluated by marginal cell-free layer and flow resistance." Am.J.Physiol.271. H2454-H2461 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tateishi,N.,Suzuki,Y..Soutani,M.et al.: "Two-dimensional imaging of microvascular network,expressed by oxygen saturation and concentration of flowing erythrocytes." Microcirculation Annual(M.Tsuchiya et al.eds). 1996. 99-100 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tateishi,N.,Suzuki,Y.and Maeda,N.: "Oxygen saturation and erythrocyte concentration plane imaging of flowing erythrocytes in microvascular network." 6th World Congress for Microcirculation(Munich). 213-216 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Maeda,N.: "Erythrocyte rheology in microcirculation." Jpn.J.Physiol.46. 1-14 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tateishi, N., et al.: "Spectrophotometric analysis of oxygen release at single microvessel level." “Progress in Microcirculation Research" Ed. by H. Niimi, et al.231-234 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tateishi, N., et al.: "Flow dynamics of erythrocytes in microvessels of isolated rabbit mesentery: Cell-free layer and flow resistance." J. Biomechanics. 27. 1119-1125 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Soutani, M., et al.: "Quantitative evaluation of flow dynamics of erythrocytes in microvessels: Influence of erythrocyte aggregation." Am. J. Physiol.268. H1959-H1965 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tateishi, N., et al.: "Plane imaging of oxygen saturation of flowing erytrhocytes in microvascular networks." Biorheology. 32. 285 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tateishi, N., et al.: "Oxygen release from single microvessels in hypoxic condition." “Adaptation of Tissue Oxygen Transport to Hypoxia" Ed. by Takahashi, E. and Yamaguchi, K.25-30 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tateixhi, N., et al.: "Plane imaging of oxygen saturation of flowing erythrocytes in microvascular network." Microcirc. Annu. 1995. 195-196 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Maeda N, Shiga T.: "Velocity of exygen transfer and erythrocyte rheology." News Physiol. Sci.9. 22-27 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tateishi N. et al.: "Flow dynamics of erythrocytes in microvessels of isolated rabbit mesentery:Cel-free laver and flow resistance" J. Biomechanics. 27. 1119-1125 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Maeda N. et al.: "Decreased deformability of red cells in refractory anemia and the abnormality of the mombrane skeleton." Biorheology. 31. 395-405 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Muralidharan E. et al.: "Simultaneous influence of erythrocyte defermability and macromolecules in the meium on erythrocyte aggregation" Biochim. Biophys. Acta. 1194. 225-263 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Soutani,M.et al.: "Quantitative evaluation of the flow dynamics of erythrocytes in microvessels:Influence of erythrocyte aggregation." Am.J. Physiol.(in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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