| Project/Area Number |
06557024
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Immunology
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| Research Institution | Science University of Tokyo |
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Principal Investigator |
NAKAYAMA Toshinori Science University of Tokyo, Research Institute for Biological Sciences, Associate Professor, 生命科学研究所, 助教授 (50237468)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Kazuo National institute of Health Japan, Researcher, 研究員 (20192130)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | calcium / oscillation / コンピューター画像解析 / カルシウムイオン / 刺激伝達系 / リンパ球 / カルシウムオシレーション / コンピュータ画像解析 |
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| Research Abstract |
We have tried to established a system, in which intracellular signaling event are quantitatively detected and visualized on the computer displays. The materials used are lymphocytes, thymocytes, and thymic organs. Those cells were atimulated with anti-T cell antigen receptor antibodies or PMA & ionomycin. The intra-cellular free calcium ion concentration ([Ca2+]i) was measured in the stimulated cells for 15 min.to 1 hour. We could detect a rapid increase and long-lasting oscillations of [Ca2+]i quantitatively. These data obtained by using this grant are published in several journals. The measurement was also succeeded in thymocytes presented in the organ cultured thymus. This assasy system is one of the novel experimental detection system, and provide a possibility for analyzing intra-cellular signaling events in live organs.
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