| Project/Area Number |
06557039
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Fukui Medical School |
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Principal Investigator |
AZUMA Takeshi (1995) Fukui Medical School, Assistant Prof., 医学部附属院, 講師 (60221040)
郡 大裕 (1994) 福井医科大学, 医学部, 助教授 (70079820)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Michihiro Nihon Kohden, Chief Assistant, 開発部, 副部長
東 健 福井医科大学, 医学部, 助手 (60221040)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | Helciobacter pylori / Urease / Diagnosis / 内視鏡診断 / H.pylori |
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| Research Abstract |
An endoscopic tube-tip type urease sensor was developed for the purpose of on-site detection of H.pylori using a pH-sensitive field effect transistor (pH-FET). The sensor is composed of an endoscopic-tube connected to a feed pump with a urea solution and a pH-FET attached to a sensor holder at the open end of the tube. The urease activity on the gastric wall can be measured by bringing the sensor into contact with the gastric wall just after replacing gastric juice with the urea solution. The presence of H.pylori causes a pH change in the urea solution inside the sensor holder in 20 seconds due to the strong urease activity of this bacterial species. After measurement, the sensor is detached from the wall, and washed with urea solution by turning on the urea pump. A measurement at one site is completed within 30 seconds. Repetition of the procedure makes multi-site measurements possible. It was found that clinical sensitivity and specificity were 92.3% and 89.7%, respectively, using standard bacteriological testing results as a reference.
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