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Molecular cloning of ATP-sensitive K^+ channels and development of the method evaluating K^+ channel openers.

Research Project

Project/Area Number 06557044
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Circulatory organs internal medicine
Research InstitutionOsaka University

Principal Investigator

KURACHI Yoshihisa  Faculty of Medicine, Osaka University, Professor, 医学部, 教授 (30142011)

Co-Investigator(Kenkyū-buntansha) YAMADA Mitsuhiko  Faculty of Medicine, Osaka University, Assistant Professor, 医学部, 助手 (10263237)
TAKUMI Toru  Faculty of Medicine, Osaka University, Assistant Professor, 医学部, 助手 (00222092)
HORI Masatsugu  Faculty of Medicine, Osaka University, Professor, 医学部, 教授 (20124779)
堀尾 嘉幸  大阪大学, 医学部, 講師 (30181530)
高橋 尚彦  大阪大学, 医学部, 助手 (30263239)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,400,000 (Direct Cost: ¥15,400,000)
Fiscal Year 1996: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥6,900,000 (Direct Cost: ¥6,900,000)
KeywordsATP-sensitive potassium channels / heart / smooth muscle / cloning / sulfonylurea receptor / ATP / K^+ channel opener / culture cell / スルフオニル尿素受容体 / カリウム / チャネル / ATP感受性チャネル / スルフォニルウレア / cDNA / パッチクランプ / K_<ATP>チャネル / NDP / G蛋白
Research Abstract

ATP-sensitive potassium channels (K_<ATP>), which represent a family of K^+ channels inhibited by intracellular ATP,have been found in various tissues including heart, pancreatic beta-cells, and smooth muscle. K_<ATP> in different tissues exibit considerable variation in responce to K^+ channel openers (KCO). In the present study we investigated the molecular difference of K_<ATP> channels and establilsh a in vitro screening system to categorize KCOs and also for developping new KCOs. K_<ATP> consists of two different subunits, K^+ channel subunit and sulfonylurea receptor (SUR) subunit. We have cloned BIR/Kir6.2 and uK_<ATP>/Kir6.1, which are K^+ channel subunits. Then we isolated SUR2A and SUR2B cDNAa for SUR subunits. SUR2B was considered as a splice variant of SUR2A and had a very similar sequence to that of SUR1 in its carboxy terminal 42 amino acid residues. We expressed these cDNAs in HEK293T cells and assayd the cells with patch clamp method. When SUR2A and BIR were transfected in HEK cells, very similar channels to those in cardiac muscle were constructed. On the other hand, a combination of SUR2B and uK_<ATP> represents a K_<ATP>, whose properties were almost same as those of vascular smooth muscle K_<ATP>. Pinacidil could activate both SUR2A/Bir and SUR2B/uK_<ATP> channels. Small amount of diazoxide was able to open the SUR2B/uK_<ATP> channels but not those of SUR2A/Bir. Thus, using cloned K_<ATP> channel subunits and HEK293T cells, we reconstituted K_<ATP> channels of cardiac and vascular smooth muscle and evaluated the effects of KCOs on these channels. This in vitro system will be used to categorize KCOs and also develop new drugs.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (106 results)

All Other

All Publications (106 results)

  • [Publications] Yamada M,: "Spermine gates inward-rectifying muscarinic but not ATP-sensitive K+ channels in rabbit atrial myocytes : intracellular substance-mediated mechanismof inwardrectification." The Journal of Biological Chemistry. 270. 9289-9294 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamashita T: "Flecainide inhibits the transient outward current in atrial myocytes isolated from the rabbit heart." The Journal of Pharmacology and Experimental Therapeutics. 274. 315-321 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hazama H: "Effects of azelastine on membrane currents in tracheal smooth muscle cells isolated from the guinea-pig." European Journal of Pharmacology. 259. 143-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hazama H: "Effects of azelastine on membrane currents in tracheal smooth muscle cells isolated from the guinea-pig." European Journal of Pharmacology. 259. 143-150 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takumi T: "A novel ATP-dependent inward rectifier potassium channel is expressing predominantly in glial cells." The Journal of Biological Chemistry. 270. 16339-16346 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inanobe A: "Gβγ directly binds to the carboxyl terminus of the G protein-gated muscarinic K+ channel,GlRK1." Biochemical and Biophysical Research Communications. 212. 1022-1028 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T: "Ionic basis of neurokinin A-induced depolarization in single smooth musckle cells isolated from the guinea-pig trachea." Pflugers Archiv European Journal of Physiology. 403. 552-562 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inanobe A: "Immunological and physical characterization of the brain G protein-gated muscarinic potassium channel." Biochemical and Biophysical Research Communications. 217. 1238-1244 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T: "17β-Estradiol inhibits the voltage-dependent L-type Ca2+ currents in aortic smooth muscle cells.European" Journal of Pharmacology. 294. 625-635 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shen WK: "Mechanisms of adenosine-mediated actions of cellular and clinical cardiac electrophysiology." Mayo Clin Proc. 70. 274-291 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A: "Cardiac ATP-sensitive K+ channels : regulation by intracellular nucleotides and K+ channel-opening drugs." American Journal of Physiology. 269. C525-C545 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y: "Gprotein regulation of cardiac muscarinic potassium channel." American Journal of Physiology. 269. C821-C830 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N: "Molecular and functional heterogeneity of inward recifier potassium channels in brain and heart." Heart and Vessels Suppl. 6. 8-11 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ismoto S.: "A novel ubiquitously-distributed isoform of GIRK2(GIRK2B) enhances GIRK1 expression of the G-protein-gated K+ current in Xenopus oocytes." Biochemical and Biophysical Research Communications. 218. 286-291 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horio Y.: "Differential distribution of classical inwardly rectifying potassium channel mRNAs in the brain ; comparison of IRK2 with IRK1 and IRK3." FEBS Letters. 379. 239-243 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morishige K.: "G Protein-gated K+ channel(GIRK1) protein is expressed presynaptically in the paraventricular nucleus of the hypothalamus." Biochemical and Biophysical Research Communications. 220. 300-305 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A.: "Actin microfilament-distrupters enhance KATP channel opening in patches from guinea-pig cardiomyocytes." Journal of Physiology. 492.2. 395-404 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito M.: "Immunolocalization of an inwardly rectifying K+ channel,KAB-2(Kir4.1),in the basolateral membrane of renal distal tubular epithelia." FEBS Letters. 388. 11-15 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamashita T.: "Competition between Mg2+ and spermine for a cloned IRK2 channel expressed in a human cell line." Journal of Physiology. 493. 143-156 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S.: "A novel sulfonylurca receptor forms with BIR (Kir6.2) a smooth muscle type ATP-sensitive K+ channel." The Journal of Biological Chemistry. 271. 24321-24324 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y.: "Molecular and functional heterogeneity of inward rectifier potassium channels in brain and heart." Journal of Cardiac Failure. 2(4S). S59-S62 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito H.: "Phosphorylation-independent inhibition by intracellular cyclic nucleotides of brain inwardly rectifying K+ current expressed in Xenopus oocytes." FEBS Letters. 402. 12-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada M.: "Sulfonylurea receptor 2B and Kir6.1 form a sulfonilurea-sensitive but ATP-insensitive K+ channel." Journal of Physiology. 499.3. 715-720 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horio Y,: "Clustering and enhanced activity of an inwardly rectifying potassium channel,Kir4.1 by an anchoring protein,PSD-95SAP 90 family." The Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hibino H.: "An ATP-dependent inwardly rectifying potassium channel,KAB-2 (Kir4.1),in cochlear stria vascularis of inner ear : its specific subcellular localization and correlation with the formation of endocochlear potential." The Journal of Physiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S.: "Inwardly rectifying potassium channels : their molecular heterogeneity and function." Japaness Journal of Physiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S.: "Molecular aspects of ATP-sensitive K+ channels in the cardiovascular system." Journal of Cardiovascular Electrophysiology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takumi T.: "Assignment of the murine inward rectfier potassium channel Irk2 (Kir2.2) gene to the central region of mouse chromosome 11." Genomics. 37. 270-272 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kondo C.: "Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP-dependent K+ channel,ROMK6(Kir1.1f)." FEBS Letters. 399. 122-126 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hosoya Y.: "A functional model for G protein activation of the muscarinic K+ channel in guinea pig atrial myocytes." Journal of General Physiology. 108. 485-495 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hazama H.: "Neurokinin a and Ca2+ current induce Ca2+ -activated Cl- currents in guinea-pig tracheal myocyres." Journal of Physiology. 492(2). 377-393 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T.: "Endothelin-1 and vasopressin activate Ca2+ -permeable non-selective cation channels in aortic smooth muscle cells : mechanism of receptor-mediated Ca2+ influx." Journal of Molecular and Cellular Cardiology. 28. 707-722 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horio Y.: "Clustering and enhanced activity of an inwardly rectifying potassium channel,Kir4.1,by an anchoring protein,PSD-95/SAP90" The Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y: "In Physiology and Pathophysiology of the Heart (3rd ed.) ed by Sperelakis N." Kluwer Academic Publishers,Boston, 789-800 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N: ""Recent Progress in Electropharmacology of the Heart" ed by Toyama J,Hiraoka M,Kodama I," CRC Press,Inc.,Boca Raton,New York,London,Tokyo, 63-71 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N,: ""Molecular and Cellular Mechanisms of Cardiovascular Regulation" (Eds,M.Endoh,M.Morada,H.Scholz,T.Iijima)," Springer-Verlag Tokyo., 59-67(452) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morad M: "Molecular Physiology and Pharmacology of Cardiac Ion Channels and Transporters.Morad M,Ebashi S,Trautwein W,Kurachi Y.eds." Kluwer Academic Publishers,Dordrecht/Boston/London, (593) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y.: ""Molecular Physiology and Pharmacology of Cardiac Ion Channels and Transporters"(Eds M.Morad,S.Ebashi,W.Trautwein and Y.Kurachi)" Kluwer Academic Publishers Dordrecht/Boston/London, 177-186(593) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y.: "Molecular Physiology and Pharmacology of Cardiac Ion Channels and Transporters(Eds M.Morad,S.Ebashi,W.Trautwein and Y.Kurachi)" Kluwer Academic Publishers, 305-312(593) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A: "Molecular Physiology and Pharmacology of Cardiac Ion Channels and Transporters(Eds M.Morad,S.Ebashi,W.Trautwein and Y.Kurachi)" Kluwer Academic Publishers., 555-561(593) (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada M,Kurachi Y: "Spermine gates inward-rectifying muscalinic but not ATP-censitive K^+ channels in rabbit atrial myocytes : intracellular substance-mediated mechanism of inward rectification." The Journal of Biological Chemistry. 270. 9289-9294 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamashita T,Nakajima T., Hamada E,Hazama H,Omata M,Kurachi Y: "Flecainide inhibits the transient outward current in atrial myocytes isolated from the rabbit heart." The Journal of Pharmacological and Experimental Therapeutics. 274. 315-321 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takumi T,Ishii T,Horio Y,Morishige K,Takahashi N,Yamada M,Yamashita T,Kiyama H,Sohmiya K,Nakanishi S,Kurachi Y: "A novel ATP-dependent inward rectifier potassium channel expressed predominantly in glial cells." The Journal of Biological Chemistry. 270. 16339-16346 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inanobe A,Morishige K,Takahashi N,Ito H,Yamada M,Takumi T,Nishina H,Takahashi K,Kanaho Y,Katada T,Kurachi Y: "G_<betagamma> directly binds to the carboxyl terminus of the G protein-gated muscarinic K^+ channel, GIRK1." Biochemical and Biophysical Research Communications. 212. 1022-1028 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T,Hazama H,Hamada E,Omata M,Kurachi Y: "Ionic basis of neurokinin A-induced depolarization in single smooth muscle cells isolated from the guinea-pig trachea." Pflugers Archiv European Journal of Physiology. 430. 552-562 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inanobe A,Ito H,Ito M,Hosoya Y,Kurachi Y: "Immunological and physical characterization of the brain G protein-gated muscarinic potassium channel." Biochemical and Biophysical Research Communications. 217. 1238-1244 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T,Kitazawa T,Hamada E,Hazama H,Omata M,Kurachi Y: "17beta-Estradiol inhibits the voltage-dependent L-type Ca^<2+> currents in aortic smooth muscle cells." Eurpean Jounral of Pharmacology. 294. 625-635 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shen WK,Kurachi Y: "Mechanisms of adenosine-mediated actions on cellular and clinical cardiac electrophysiology." Mayo Clinic Proceedings. 70. 274-291 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A,Jahangir A,Kurachi Y: "Cardiac ATP-sensitive K+ channels : Regulation by intracellular nucleotides and potassium channel opening drugs." American Journal of Physiology. 269. C525-C545 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y: "G protein regulation of cardiac muscarinic potassium channels." American Journal of Physiology. 269. C821-C830 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N,Morishige K,Yamada, Takumi T,Kurachi Y: "Molecular and functional heterogeneity of inward rectifier potassium channels in brain and heart." Heart and Vessels Suppl.9. 8-11 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S,Kondo C,Takahashi N,Matsumoto T,Yamada M,Takumi T,Horio Y,Kurachi Y: "A novel ubiquitously-distributed isoform of GIRK2 (GIRK2B) enhances GIRK1-expression of the G-protein-gated K^+ current in Xenopus oocytes." Biochemical and Biophysical Research Communications. 218. 286-291 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horio Y,Morishige K,Takahashi N,Kurachi Y: "Differential distribution of classical inwardly rectifying potassium channel mRNAs in the brain : comparison of IRK2 with IRK1 and IRK3." FEBS Letters. 379. 239-243 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakajima T,Hazama H,Hamada E,Wu SN,Igarashi K,Yamashita T,Seyama Y,Omata M,Kurachi Y: "Endothelin 1 and vasopressn actvate Ca^<2+>-permeable non-selective catiion channels in aortic smooth muscle cells : Mechanism of receptor-mediated Ca^<2+> influx." Journal of Molecular and Cellular Cardiology. 28. 707-722 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A,Kurachi Y: "Actin microfilament-distrupters activate K_<ATP> chanels by antagonizing ATP-dependent gating in patches from guinea-pig cardiomyocytes." Journal of Physiology. 492. 395-404 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hazama H,Nakajima T,Hamada E,Omata M,Kurachi Y: "Neurokinin A and Ca2+ current iinduce Ca2+-activated Cl-currents in guinea-pig tracheal myocytes." Journal of Physiology. 492. 377-393 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamashita T,Horio Y,Yamada M,Takahashi N,Kondo C.Kurachi Y: "Competition between Mg^<2+> and spermine for cloned IRK2 channel expressed in a human cell line." Journal of Physiology. 493. 143-156 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morishige K,Inanobe A,Takahashi N,Yoshimoto Y,Kurachi H,Miyake A,Maeda T,Kurachi Y: "G protein-gated K+ channel (GIRK1) protein is expressed presynaptically in the paraventricular nucleus of the hypothalamus." Biochemical and Biophysical Research Communications. 220. 300-305 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito M,Inanobe A,Horio Y,Hibino H,Isomoto S,Ito HH,Mori K,Tonosaki A,Tomoike H,Kurachi Y: "Immunolocaglization of an inwardly rectifying K+ channel, K_<AB>-2 (Kir4.1), in the basolateral membrane of renal distal ubular epithelia." FEBS Letters. 338. 11-15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S,Kondo C,Yamada M,Matsumoto S,Higashiuchi O,Horio Y,Matsuzawa YY,Kurachi Y: "A novel suofonylurea receptor forms with BIR (Kir6.2) a smooth muscle type ATP-sensitive K+ channel." The Journal of Biological Chemistry. 271. 24321-24324 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takumi T,Tsuji L,Kondo C,Takahashi N,Morishhige K,Copeland NG,Gilbert DJ,Jenkins NA,Kurachi Y: "Assignment of the murine in ward rectifier potassium channel ir-k2 (Kir2.2) gene to the central region of mouse chromosome ll." Genomics. 37. 270-2272 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hosoya Y,Yamada M,Ito H,Kurachi Y: "A functional model for G protein activation of the muscarinic K+ channel in guinea pig atrial myocytes : Spectral analysis of the effect of TP on single-channel kinetics." The Journal of General Physiology. 108. 485-495 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kondo C,Isomoto S,Matsumoto S,Yamada M,Horio Y,Yamashita S,Takemura-Kameda K,Matsuzawa Y,Kurachi Y: "Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATO-dependent K+ channel, ROMK6 (Kir1.1f)." FEBS Letters. 399. 122-126 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y Takahashi N: "Molecular and functional heterogeneity of iinward rectifier potassium channels in brain and heart." Journal of Cardic Failure. 2. 59-62 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ito H,Tsuchimochi H,Tada Y,Kurachi Y: "Phosphorylation-independent inhibition by intracellular cyclic nucleotides of brain inwardly rectifyting K+ current expressedin Xenopus oocytes." FEBS Letters. 402. 12-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada M,Isomoto S,Matsumoto S,Kondo C,Shindo T,Horio Y,Kurachi Y: "Sulfonylurea receptor 2B and Kir6.1 form a sulfonilurea-sensitive but ATP-insensitive K+ channel." Journal of Physiology. 499. 715-720 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horio Y,Hibino H,Inanobe A,Yamada M,Ishii M,Tada Y,Sato E,Hata Y,Takai Y,Kurachi Y: "Clustering and enhanced activity of an inwardly rectifying potassium channel, Kir4.1. by an anchoring protein, PSD-95/SAP 90 family." The Journal of Biological Chemistry. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hibino H,Horio Y,Inanobe A,Doi K,Yamada M,Gotow T,Uchiyama Y,Kubo T,Kurachi Y: "An ATP-dependent inwardly rectifying potassium channel, K_<AB>-2 (Kir4.1), in cochlear stria vascularis of inner ear : its specific subcellular localization and correlation with the formation of endocochlear potential." The Journal of Neuroscience. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S,Kondo C,Kurachi Y: "Inwardly rectifying potassium channe Is : their molecular heterogeneity and function" Japanese Journal of Physiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isonoto S,Kurachi Y: "Molecular aspects of ATP-sensitive K+ channels in the cardiovascular system." Journal of Cardiovascular Electrophysiology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y: Adenosine and adenine nucleotides action on cardiac cellular excitation. In "Physiology and pathophysiology of the heart" ed by Sperelaxis N,chapter 41. Martinus Nijhoff, Boston, 789-800 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N,Morishige K,Yamada M,Kondo C,Kurachi Y: Molecular and functional diversity of inward rectifier potassium channels in brain and heart. In "Recent Progress in Electropharmacology of the heart" ed by Toyama J,Hiraoka M,Kodama I. 63-71 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takahashi N,Morishige K,Yamada M,Kondo C,Kurachi Y: Molecular and functional heterogeneityy of inward rectifier potassium channels in the brain and heart. In "Molecular and Cellular Mechanisms of Cardiovascular Regulation" ed by Endoh M,Morad M,Scholz H,and Iijima T. 59-67 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y: Muscarinic and purinergic regulation of cardiac K+ channels. In "Molecular Physiology and Pathophysiology of Cardiac Ion Channels and Transporters" ed by M Morad, S Ebashi, W Trautwein, Y Kurachi. Chapter 15. Kluwar, Dordrecht, 177-186 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kurachi Y,Terzic A: Findlay I Pharmacological regulation of the cardiac ATP-sensitive K+ channel. In "Molecular Physiology and Pathophysiology of Cardiac Ion Channels and Transporters" ed by M Morad, S Ebashi, W Trautwein, Y Kurachi. Chapter 25. Kluwar, Dordrecht, 305-312 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terzic A,Kurachi Y: Regulation of intracellular protons : role of Na/H exchange in cadiac myocytes. In "Molecular Physiology and Pathophysiology of Cardiac Ion Channels and Transporters" ed by M Morad, S Ebashi, W Trautwein, Y Kurachi. Chapter 46. Kluwar, Dordrecht, 555-561 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Isomoto S.: "A novel ubiquitously-distributed isoform of GIRK2(GIRK2B) enhances GIRK1 expression of the G-protein-gated K+ current in Xenopus oocytes." Biochemical and Biophysical Research Communications. 218. 286-291 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Horio Y.: "Differential distribution of classical inwardly rectifying potassium channel mRNAs in the brain; comparison of IRK2 with IRK1 and IRK3." FEBS Letters. 379. 239-243 (1996)

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      1996 Annual Research Report
  • [Publications] Morishige K.: "G Protein-gated K+ channel (GIRK1) protein is expressed presynaptically in the paraventricular nucleus of the hypothalamus." Biochemical and Biophysical Research Communications. 220. 300-305 (1996)

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      1996 Annual Research Report
  • [Publications] Terzic A.: "Actin microfilament-distrupters enhance KATP channel opening in patches from guinea-pig cardiomyocytes." Journal of Physiology. 492.2. 395-404 (1996)

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      1996 Annual Research Report
  • [Publications] Ito M.: "Immunolocalization of an inwardly rectifying K+ channel,KAB-2(Kir4.), in the basolateral membrane of renal distal tubular epithelia." FEBS Letters. 388. 11-15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamashita T.: "Competition between Mg2+ and spermine for a cloned IRK2 channel expressed in a human cell line." Journal of Physiology. 493. 143-156 (1996)

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      1996 Annual Research Report
  • [Publications] Isomoto S.: "A novel sulfonylurea receptor forms with BIR(Kir6.2)a smooth muscle type ATP-sensitive K+ channel." The Journal of Biological Chemistry. 271. 24321-24324 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kurachi Y.: "Molecular and functional heterogeneity of inward rectifier potassium channels in brain and heart." Journal of Cardiac Failure. 2(4S). S59-S62 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumi T.: "Assignment of the murine inward rectifier potassium channel Irk2(Kir2.2) gene to the central region of mouse chromosome 11." Genomics. 37. 270-272 (1996)

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      1996 Annual Research Report
  • [Publications] Kondo C.: "Cloning and functional expression of a novel isoform of ROMK inwardly rectifying ATP-dependent K+ channel,ROMK6(Kirl.lf)." FEBS Letters. 399. 122-126 (1996)

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      1996 Annual Research Report
  • [Publications] Hosoya Y.: "A functional model for G protein activation of the muscarinic K+ channel in guinea pig atrial myocytes." Journal of General Physiology. 108. 485-495 (1966)

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      1996 Annual Research Report
  • [Publications] Hazama H.: "Neurokinin a and Ca2+ current induce Ca2+ -activated Cl- currents in guinea-pig tracheal myocytes." Journal of Physiology. 492(2). 377-393 (1996)

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      1996 Annual Research Report
  • [Publications] Nakajima T.: "Endothelin-1 and vasopressin activate Ca2+ -permeable non-selective cation channels in aortic smooth muscle cells: mechanism of receptor-mediated Ca2+ influx." Journal of Molecular and Cellular Cardiology. 28. 707-722 (1966)

    • Related Report
      1996 Annual Research Report
  • [Publications] Horio Y: "Clustering and enhanced activity of an inwardly rectifying potassium channel,Kir4.1,by an anchoring protein,PSD-95/SAP90" The Journal of Biologycal Chemistry. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamada M,Kurachi Y: "Spermine gates inward-rectifying muscarinic but not ATP-sensitive K_+ channels in rabbit atrial myocytes:intracellular substance-mediated mechanism of inward rectification." J.Biol.Chem.270. 9289-9294 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamashita T,et al.: "Flecainide inhibits the transient outward current in atrial myocytes isolated from the rabbit heart." J.Pharmacol.Exp.Ther.274. 315-321 (1995)

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      1995 Annual Research Report
  • [Publications] Takumi T et al.: "A novel ATP-dependent inward rectifier potassium channe expressed predominantly in glial cells." J.Biol.Chem.270. 16339-16346 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Isomoto S et al.: "A novel ubiquitously-distributed isoform of GIRK2(GIRK2B) enhances GIRK1-expression of the G-protein gated K_+ current in Xenopus oocytes." Biochem.Biophys.Res.Commun.218. 286-291 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Terzic A et al.: "Cardiac ATP-sensitive K_+ channels:Regulation by intracellular nucleotides and potassiumchannel opening drugs." Am.J.Physiol.269. C525-C545 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kurachi Y: "G protein regulation of cardiac muscarinic potassium channel." Am.J.Physiol.269. C821-C830 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kurachi Y: "physiology and pathophysiology of the heart" Martinus Nijhoff,Boston., (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kurachi Y: "Molecular Physiology and Pathophysiology of Cardiac Ion Channels and Transporters" Kluewar(in press), (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Terzic A et al: "HOE-234,a second generation of K^+ channel opener,antagonizes the ATP-dependent gating of cardiac ATP-densitive K^+ channel." The Journal of Phamacological and Experimental Therapeutics. 268. 818-825 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Terzic A et al: "G proteins activate ATP-sensitilve K^+ channels by antagonizing the ATP-dependent gating." Neuron. 12. 885-893 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Terzic A et al: "Dualistic behavior of ATP-dependent K^+ channel towards intracellular nucloside diphosphates." Neuron. 12. 1049-1058 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Jahangir A et al.: "Intracelluar acidification and ADP enhace nicorandil induction of ATP-sensitive K^+ channel current in cardiomyocytes." Cardiovascular Research. 28. 831-835 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takahashi N et al: "Molecular cloning and functional expression of cNDA encoding a second class of inward rectifier potassium channels in the mouse brain." The Journal of Biological Chemistry. 269. 23274-23279 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamada M et al: "Spermine gates inward-rectifying muscarinic but not ATP-sensitive K^+ channels in rabbit atrial myocytes:intracellular substance-mediated mechanism of inward rectification." The Journal of Biological Chemistry. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kurachi Y: "Molecular Neurobiology:Proceeding of the second NIMH conference 14" National Institute of Mental Health,Maryland, (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kurachi Y: "Physiology and pathophysiology of the heart 12" Martinus Nijhoff,Boston, (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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