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TO DEVELOP A NEW SYSTEM TO CONTIMUOUSLY MONITOR THE FUNCTIONS AT THE CELLULAR AND MOLECULAR LEVELS OF THE VASCULAR ENDOTHELIAL AND SMOOTH MUSCLE CELLS IN VIVO.

Research Project

Project/Area Number 06557045
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Circulatory organs internal medicine
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KANAIDE Hideo  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (80038851)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Junji  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (90237727)
KOBAYASHI Sei  KYUSHU UNIVERSITY,FACULTY OF MEDICINE,ASSOCIATE PROFESSOR, 医学部, 助教授 (80225515)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥12,300,000 (Direct Cost: ¥12,300,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1994: ¥8,000,000 (Direct Cost: ¥8,000,000)
KeywordsVascular endothelial cells / vascular smooth muscle cells / measurements in vivo / cytosolic calcium concentration / fura-2 / フラ-ス
Research Abstract

1. THE DEVELOPMENT OF A NEW SYSTEM FOR MONITORING THE FUNCTIONS OF THE VASCULAR ENDOTHELIAL AND SMOOTH MUSCLE CELLS : The system we have developed has following characteristics ; (1) Using a grating and a chopper mirror driven by a stepping motor, it became possible to alternately (10 Hz) illuminate the tissue sample with 340 and 380 nm excitation lights for the measurement of intracellular calcium concentration, [Ca] i. (2) the chopper system is controlled through a personal computer by investigator designed software. (3) Fluorescence images are obtained using a chopper mirror and a SIT camera. A display flame is 640x400 dots and a 8 bit flame. (4) Fluorescence ratio (F340/F380) images can be obtained at 5 Hz. (5) It is possible to simultaneously record changes in the [Ca] i levels at 30 points in an image on the TV monitor. (6) Low cost for making the system.
2. NEW FINDINGS OBTAINED USING THIS SYSTEM,FRONT-SURFACE FLUORIMETRY AND TECHNIQUES IN MOLECULAR BIOLOGY : (1) Using the newly developed technique, the simultaneous fluorimetry of fura-2 and diaminonaphthalene, we found the [Ca] i-NO production relationships in the endothelial cells in situ. (2) Using front-sueface fluorimetry, reverse transcription polymerase chain reaction, and the renal and coronary arteries, we found that both adrenomedullin and endothelin might act as autocrine/paracrione signal transmitters in these tissues.
3. HUMANA PRESS (NJ : U.S.A) asked us to write a chapter "Measurement of [Ca] i in muscle strips using front-surface fluorimetry" of a book on "Calcium signaling protocols (edited by DG Lambert)", a part of the Methods in Molecular Biology series. It is in press.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (125 results)

All Other

All Publications (125 results)

  • [Publications] H.Aoki: "Relationship between cytosolic calcium concentration and force in the papaverine-induced relaxation of medial strips of pig" Br J Pharmacol. 111. 489-496 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Aoki: "Sensitivity of G-protein involved in endothelial-1-induced Ca2+ influx topertussis toxin in porcine endothelial cells" Br J Pharmacol. 111. 989-996 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kobayashi: "Cytosolic Ca2+ transients are not required for platelet-derived growth factor to induce cell cycle progression of" J Biol Chem. 269. 9011-9018 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] X.Chen: "Protein kinase C and protein kinase A regulate the expression of angiotensin II receptor mRNA in smooth muscle cells." Eur J Pharmacol. 267. 175-183 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Abe: "Effects of nicorandil on cytosolic calcium concentrations and on tension development in the rabbit femoral artery." J Pharmacol Exp Ther. 268. 762-771 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirakawa: "Actions of a new Ca2+ channel antagonist,CD 832,on two types of Ca2+ channels in smooth muscle." Eur J Pharmacol. 252. 267-274 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Higuchi: "Motilin induces the endothelium-dependent relaxation of smooth muscle and the elevation of cytosolic calcium in" Biochem Biophys Res Commun. 202. 346-353 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Ushio-Fukai: "The effects of a novel vasodilator LP-805,on cytosolic Ca2+ concentrations and on tension in rabbit isolated femoral" Br J Pharmacol. 113. 1173-1182 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kodama: "Myosin phosphorylation and Ca2+ sensitization in porcine coronary arterial smooth muscle stimulated with endothelin-1." Eur J Pharmacol. 288. 69-77 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Ushio-Fukai: "Endothelin-1 and endothelin-3 regulate differently vasoconstrictor responses of smooth muscle of the porcine coronary" Br J Pharmacol. 114. 171-179 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fukuizumi: "Cytolic calcium concentration-force relation during contractions in the rabbit femoral artery ; time-dependency and" Br J Pharmacol. 114. 329-338 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirakawa: "Dual regulation of L-type Ca2+ channels cy serotonin 2 receptor stimulation in vascular smooth muscle cells." Am J Physiol. 268. H544-H549 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Resting load regulates cytosolic calcium-force relationship of the contraction of bovine cerebrovascular smooth muscle." J Physiol. 484. 123-137 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Resting load regulates vascular sensitivity by a cytosolic Ca2+-insensitive Mechanism." Am J Physiol. 268. C1332-C1341 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Toyofuku: "Effects of U46619 on intracellular Ca++ concentration and tension in human umbilical artery." Am J Obstet Gynecol. 172. 1414-1421 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Hiroishi: "High D-glucose stimulates the cell cycle from G1 to the S and M phases,but has no competent effect on the GO phase,in" Biochem Biophys Res Commun. 211. 619-626 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Kureishi: "Adrenomedullin decreases both cytosolic Ca2+ concentration and Ca2+- sensitivity in pig coronary arterial smooth muscle" Biochem Biophys Res Commun. 212. 572-579 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "Evidence for the presence of endothlin ET_A receptors in endothelial cells in situ on the aortic side of porcine" Br J Pharmacol. 115. 1369-1376 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuroiwa: "Mechanism of endothelium-dependent relaxation induced by substance P in the coronary artery of the pig." Br J Pharmacol. 116. 2040-2047 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Seguchi: "Autocrine regulation of the renal arterial tone by adrenomedullin." Biochem Biophys Res Commun. 215. 619-625 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Yoshimura: "Effects of midazolam on intracellular Ca2+ and tension in airway smooth muscle." Anesthesiology. 83. 1009-1020 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Jahan: "Endothelin-1 and angiotensin II act as progression but not competence growth factors in vascular smooth muscle cells." Eur J Pharmacol. 295. 261-269 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Abe: "Some effects of nipradilol,a β-blocker possessing a nitroxy group,on smooth muscle of the pig coronary artery." Br J Pharmacol. 117. 1707-1715 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Seguchi: "The mechanism of relaxation induced by atrial natriuretic peptide in the porcine renal artery." Br J Pharmacol. 118. 343-351 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Dual regulation of cerebrovascular tone by UTP_<2U> receptor-mediated contraction and endothelium-dependent relaxation." Br J Pharmacol. 118. 847-856 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Sakihara: "Expression of calponin mRNA in porcine aortic endothelial cells." Biochem Biophys Res Commun. 222. 195-200 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "P_<2U> receptor is linked to cytosolic Ca2+ transient and release of vasorelaxing factor in bovine endothelial cells in situ." J Physiol. 492. 751-761 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "P_<2U> purinergic activation leads to the cell cycle progression from the G_1 to the S and M phases but not from the G_0 to G_1" Biochem biophys Res Commun. 222. 652-658 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fukuizumi: "The effects of calcitonin gene-related peptide on the cytosolic calcium concentration and force in the porcine coronary artery." J Pharmacol Exp Ther. 278. 220-231 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Higuchi: "CPA induces a sustained increase in 〔Ca2+〕i of endothelial in situ and relaxes porcine coronary artery." Am J Physiol. 270. H2038-H2049 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kuga: "Cell cycle-dependent expression of L-and T-type Ca2+ currents in vascular smooth muscle cells." Circ Res. 79. 14-19 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Sakihara: "The direct inhibitory effect of chlorpromazine on smooth muscle of the porcine pulonary artery." Anesthesiology. 85. 616-625 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "Expression of rhoA and rho kinase mRNAs in porcine vascular smooth muscle." Biochem Biophys Res Commun. 227. 750-754 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kai: "Halothane counteracts acetylcholine-induced increase in Ca2+ sensitivity of the contractile apparatus in airway smooth" Eur Jr Pharmacol. 315. 313-318 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "The relaxant effect of adrenomedullin on particular smooth muscles despite a general expression of its mRNA in smooth" Br J Pharmacol. 120. 193-200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] X.Chen: "Resting load modulates the myofilament Ca2+-sensitivity in rabbit cerebral arteries." J Cereb Blood Flow Metab. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Hiroishi: "The differential effects of diltiazem and nitroglycerin on cytosolic Ca2+ concentration and on force in the bovine" Invest Opthalmol Vis Sci. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Eguchi: "Mechanism of contraction induced by bradykinin in the rabbit saphenous vein." Br J Pharmacol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Niiro: "Up-regulation of RHO A and RHO-kinase mRNAs in the rat myometrium during pregnancy." Biochem Biophys Res Commun. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: "Endothelium-derived factors and vascular functions" Excerpta Medica, 10 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: "Regulation of the contractile cycle in smooth muscle" Springer-Verlag,Tokyo, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: "pathophysiology of heart failure" Kluwer Academic Publisher,Boston, 12 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Aoki: "Relationship between cytosolic calcium concentration and force in the papaverine-induced relaxation of medial strips of pig coronary artery." Br J Pharmacol. 111. 489-496 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Aoki: "Sensitivity of G-protein involved in endothelin-I-induced Ca^<2+> influx to pertussis toxin in porcine endothelial cells in situ." Br J Pharmacol. 111. 989-996 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kobayashi: "Cytosolic Ca^<2+> transients are not required for platelet-derived growth factor to induce cell cycle progression of vascular smooth muscle cells in primary culture : actions of tyrosine kinase." J Biol Chem. 269. 9011-9018 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] X.Chen: "Protein kinase C and protein kinase A regulate the expression of angiotensin II receptor mRNA in smooth muscle cells." Eur J Pharmacol. 267. 175-183 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Abe: "Effects of nicorandil on cytosolic calcium concentrations and on tension development in the rabbit femoral artery." J Pharmacol Exp Ther. 268. 762-771 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirakawa: "Actions of a new Ca^<2+> channel antagonist, CD832, on two types of Ca^<2+> channels in smooth muscle." Eur J Pharmacol. 252. 267-274 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Higuchi: "Motilin induces the endothelium-dependent relaxation of smooth muscle and the elevation of cytosolic calcium in endothelial cells in situ." Biochem Biophys Res Commun. 202. 346-353 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Ushio-Fukai.: "The effects of a novel vasodilator LP-805, on cytosolic Ca^<2+> concentrations and on tension in rabbit isolated femoral arteries." Br J Pharmacol. 113. 1173-1182 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kodama: "Myosin phosphorylation and Ca^<2+> sensitization in porcine coronary arterial smooth muscle stimulated with endothelin-1." Eur J Pharmacol. 288. 69-77 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Ushio-Fukai: "Endothelin-1 and endothelin-3 regulate differently vasconstrictor responses of smooth muscle of the porcine coronary artery." Br J Pharmacol. 114. 171-179 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fukuizumi: "Cytosolic calcium concentration-force relation during cntractions in the rabbit femoral artery : time-dependency and stimulus specificity." Br J Pharmacol. 114. 329-338 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Hirakawa: "Dual regulation of L-type Ca^<2+> channels by serotonin 2 receptor stimulation in vascular smooth muscle cells." Am J Physiol. 268. H544-H549 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Resting load regulates cytosolic calcium-force relationship of the contraction of bovine cerebrovascular smooth muscle." J Physiol. 484. 123-137 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Resting load regulates vascular sensitivity by a cytosolic Ca^<2+> insensitive mechanism." Am J Physiol. 268. C1332-C1341 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Toyofuku: "Effects of U46619 on intracellular Ca^<2++> concentration and tension in human umbilical artery." Am J Obstet Gynecol. 172. 1414-1421 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Hiroishi: "High D-glucose stimulates the cell cycle from the G_1 to the S and M phases, but has no competent effect on the G_0 phase, in vascular smooth muscle cells." Biochem Biophys Res Commun. 211. 619-626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Kureishi: "Adrenomedullin decreases both cytosolic Ca^<2+> concentration and Ca^<2+> -sensitivity in pig coronary arterial smooth muscle." Biochem Biophys Res Commun. 212. 572-579 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "Evidence for the presence of endothelin ET_A receptors in endothelial cells in situ on the aortic side of porcine aortic valve." Br J Pharmacol. 115. 1369-1376 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Kuroiwa: "Mechanism of endothelium-dependent relaxation induced by substance P in the coronary artery of the pig." Br J Pharmacol. 116. 2040-2047 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Seguchi: "Autocrine regulation of the renal arterial tone by adrenomedullin." Biochem Biophys Res Commun. 215. 619-625 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Yoshimura: "Effects of midazolam on intracellular Ca^<2+> and tension in airway smooth muscle." Anesthesiology. 83. 1009-1020 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Jahan: "Endothelin-1 and angiotensin II act as progression but not competence growth factors in vascular smooth muscle cells." Eur J Pharmacol. 295. 261-269 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Abe: "Some effects of nipradilol, a beta-blocker possessing a nitroxy group, on smooth muscle of the pig coronary artery." Br J Pharmacol. 117. 1707-1715 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Seguchi: "The mechanism of relaxation induced by atrial natriuretic peptide in the porcine renal artery." Br J Pharmacol. 118. 343-351 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "Dual regulation of cerebrovascular tone by UTP : P_<2U> receptor-mediated contraction and endothelium-dependent relaxation." Br J Pharmacol. 118. 847-856 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Sakihara: "Expression of calponin mRNA in porcine aortic endothelial cells." Biochem Biophys Res Commun. 222. 195-200 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "P_<2U> purinergic activation leads to the cell cycle progression from the G_1 to the S and M phases but not from the G_0 to G_1 phase in vascular smooth muscle cells in primary culture." Biochem Biophys Res Commun. 222. 652-658 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Miyagi: "P_<2U> receptor is linked to cytosolic Ca^<2+> transient and release of vasorelaxing factor in bovine endothelial cells in situ." J Physiol. 492.3. 751-761 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Fukuizumi: "The effects of calcitonin gene-related peptide on the cytosolic calcium concentration and force in the porcine coronary artery." J Pharmacol Exp Ther. 278. 220-231 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Higuchi: "CPA induces a sustained increase in [Ca^<2+>] i of endothelial cells in situ and relaxs porcine coronary artery." Am J Physiol. 270. H2038-H2049 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kuga: "Cell cycle-dependent expression of L-and T-type Ca^<2+> currents in vascular smooth muscle cells." Circ Res. 79. 14-19 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] C.Sakihara: "The direct inhibitory effect of chlorpromazine on smooth muscle of the porcine pulmonary artery." Anesthesiology. 85. 616-625 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "Expression of rhoA and rho kinase mRNAs in porcine vascular smooth muscle." Biochem Biophys Res Commun. 227. 750-754 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Kai: "Halothane counteracts acetylcholine-induced increase in Ca^<2+> sensitivity of the contractile apparatus in airway smooth muscle." Eur Jr Pharmacol. 315. 313-318 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J.Nishimura: "The relaxant effect of adrenomedullin on particular smooth muscles despite a general expression of its mRNA in smooth muscle, endothelial and epithelial cells." Br J Pharmacol. 120. 193-200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] X.Chen: "Resting load modulates the myofilament Ca^<2+> -sensitivity in rabbit cerebral arteries." J Cereb Blood Flow Metab. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] G.Hiroishi: "The differential effects of diltiazem and nitroglycerin on cytosolic Ca^<2+> concentration and on force in the bovine ophthalmic artery." Invest Ophthalmol Vis Sci. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] D.Eguchi: "Mechanism of contraction induced by bradykinin in the rabbit saphenous vein." Br J Pharmacol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N.Niiro: "Up-regulation of RHO A and RHO-kinase mRNAS in the rat myometrium during pregnancy." Biochem Biophys Res Commun. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: Mechanisms of endothelin-induced contraction and relaxation of vascular smooth muscle. In : Tomoh Masaki (ed) : Endothelium-derived factors and vascular functions.Excerpta Medica, 61-70 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: Cytosolic calcium concentration-force relation in vascular smooth muscle. In : Nakano T,D.J.Hartshorne (ed) : Regulation of the contractile cycle in smooth muscle.Springer-Verlag Tokyo, 61-72 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kanaide: The cytosolic calcium-force relation of vascular smooth muscle during the contraction and the relaxation. In : Dhalla NS,Singal PK,Takeda N,Beamish RE (ed) : Pathophysiology of heart failure. Kluwer Academic Publisher, Boston., 367-378 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Jahan: "Endothelin 1 and angiotensin II act as progression but not cpmpetence growth factors in vascular smooth muscle cells." Eur J Pharmacol. 295. 261-269 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Abe: "Some effects of nipradilol,a β-blocker possessing a nitroxy group,on smooth muscle of the pig coronary artery." Br J Pharmacol. 117. 1707-1715 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Seguchi: "The mechanism of relaxation induced by atrial natriuretic peptide in the porcine renal artery" Br J Pharmacol. 118. 343-351 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Miyagi: "Dual regulation of cerebrovascular tone by UTP:P_<2U> receptormediated contraction and endothelium-dependent relaxation." Br J Pharmacol. 118. 847-856 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] C.Sakihara: "Expression of calponin mRNA in porcine aortic endothelial cells." Biochem Biophys Res Commun. 222. 195-200 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Miyagi: "P_<2U> purinergic activation leads to the cell cycle progression from the G_1 to the S and M phases but not the G_0 to G_1 phasein vascular smooth muscle cells in primary culture." Biochem Biophys Res Commun. 222. 652-658 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Miyagi: "P_<2U> receptor is linked to cytosolic Ca^<2+> transient and release of of vasorelaxing factorin bovine endothelial cells in situ." J Physiol. 492.3. 751-761 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Fukuizumi: "The effects of calcitonin gene-related peptide on the cytosolic calcium concentration and force in the porcine coronary artery." J Pharmacol Exp Ther. 278. 220-231 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Higuchi: "CPA induces a sustained increase in(Ca^<2+>)i of endothelial cells in situ and relaxes porcine coronary artery." Am J Physiol. 270. H2038-H2049 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kuga: "Cell cycle-dependent expression of L- and T-type Ca^<2+> currents invascular smooth muscle cells." Circ Res. 79. 14-19 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] C.Sakihara: "The direct inhibitory effect of chlorpromazineon smooth muscle of the porcine pulmonary artery." Anesthesiology. 85. 616-625 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Nishimura: "Expression of rhoA and rho kinase mRNAs in porcine vascular smooth muscle." Biochem Biophys Res Commun. 227. 750-754 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kai: "Halothane counteracts acetylcholine-induced increase in Ca^<2+> sensitivity of the contractile apparatus in air way smooth muscle." Eur J Pharmacol. 315. 313-318 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] X.Chem: "Resting load modulates the myofilament Ca^<2+> -sensitivity in rabbit cerebral arteries." J Gereb Blood Flow Metab. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] G.Hiroishi: "The differential effects of diltiazem and nitroglycerin on cytosolic Ca concentration and on force in the bovine ophthalmic artery." Invest Ophthalmolol Vis Sci. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Nishimura: "Adrenomrdullin relaxes particular smooth muscles despite a general expression of its mRNA in smooth muscle,endothelial and epithelial cells" Br J Pharmacol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] D.Eguchi: "Mechanism of contraction induced by bradykinin in the rabbit saphenous vein." Br J Phaemacol. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Niiro: "Up-regulation of RHO A and RHO-Kinase mRNA in the rat myometrium during pregnancy." Biochem Biophys Res Commun. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Ushio-Fukai: "Cytosolic calcium concentration-force relation during contractions in the rabbit femoral artery" Br J Pharmacol. 114. 329-338 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Hirakawa: "Dual regulation of L-type Ca^<2+> channels by serotonin 2 recepror stimulation in vascular smooth muscle cells" Am J Physiol. 268. H544-H549 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Miyagi: "Resting load regulates cytosolic calcium-force relationship of the contraction of bovine cerebrovascular smooth muscle" J Physiol. 484. 123-137 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Miyagi: "Resting load regulates vascular sensitivity by a cytosolic Ca^<2+> -insensitive mechaism" Am J Physiol. 268. C1332-C1341 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Toyofuku: "Effects of U46619 on intracellular Ca^<++> concentration and tension in human umbilical artery" Am J Obstet Gynecol. 172. 1414-1421 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] G.Hiroishi: "High-D-glucose stimulates the cell Cycle from the G1 to S and M phases,but has no competent effect on the G0 phase,in vascular" Biochem biophys Res Commun. 211. 619-629 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Kureishi: "Adrenomedullin decreases both cytosolic Ca^<2+> concentration and Ca^<2+> sensitivity in pig coronary arterial smooth muscle." Biochem Biophys Res Commun. 212. 572-579 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] J.Nishimura: "Evidence for the presence of endothelin ET_A receptors in endothelial cells in situ of aortic side of porcine aortic valve" Br J Pharmacol. 115. 1369-1376 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Kuroiwa: "The mechanism of endothelium-dependent relaxation induced by substance P in the porcine coronary artery" Br J Pharmacol. 116. 2040-2047 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Seguchi: "Autocrine regulation of the renal arterial tone by adrenomedullin" Biochem Biophys Res Commun. 215. 619-625 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Yoshimura: "The effects of midazolam on intracellular Ca^<2+> and tension in airway smooth muscle" Anesthesiology. 83. 1009-1020 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Jahan: "Both endothelin-1 and angiotensin-II act as progression growth factors but not competence growth factors in vascular smooth muscle cells in primary culture" Eur J Pharmacol. 295. 261-269 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Miyagi: "Nucleotide receptor links to cytosolic calcium transient and release of vasorelaxint factor in bovine endothelial cells in situ" J Pyhsiol. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Higuchi: "Cyclopiazonic acid induces a sustained increase in the intracellular Ca^<2+> concentration of endothelial cells on the porcine aortic valve and a relaxation of the porcine coronary artery" Am J Physiol. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Abe: "Some effects of nipradilol,a β-blocker possessing anitroxy group,on smooth muscle of the pig coronary artery" Br J Pharmacol. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Aoki: "Sensitivity of G-protein involved in endothelin-1-induced Ca^<2+> influx to pertusis toxin in porcine endothelial cells in situ." Br J Pharmacol. 111. 989-996 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Kobayashi: "Cytosolic Ca^<2+> transients are not required for platelet-derived gorwth factor to induce cell cycle progression of vascular smooth" J Biol Chem. 269. 9011-9018 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Abe: "Effects of nicorandil on cytosolic calcium concentrations and on tension development in the rabbit femoral artery" J Pharmacol Exp Ther. 268. 762-771 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Ushio-Fukai: "The effects of novel vasodilator LP-805,on cytosolic Ca^<2+> concentrations and tension in rabbit isolated femoral arteries." Brit J Pharmacol. 113. 1173-1182 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kodama: "Myosin phosphorylation and Ca^<2+> sensitization in porcine coronary arterial smooth muscle stimulated with endothelin-1" Eur J Pharmacol. 288. 69-77 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Ushio-Fukai: "Endotheli-1 and endothelin-3 regulate differently vasoconstrictor responses of smooth muscle of the porcine coronary artery." Brit J Pharmacol. 114. 171-179 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Kanaide: "Pathophysiology of heart failure," Kluwer Academic Publisher, Boston (in press),

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Kanaide: "Regulation of the contractile cycle in smooth muscle." Springer-Verlag,Tokyo(in press),

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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