| Project/Area Number |
06557050
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
TAKIGAWA Masahiro Hamamatsu University School of Medicine. Dermatology, Professor, 医学部, 教授 (80115873)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMOTO Masafumi Suntory Institute for Biomedical Reseach Pharmaceutical Reseach Laboratories 1,, 主席研究員
KOHNO Michiaki Gifu Pharmaceutical University. Biology, Associate Professor, 薬学部, 助教授 (00027335)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥14,300,000 (Direct Cost: ¥14,300,000)
Fiscal Year 1995: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥8,000,000 (Direct Cost: ¥8,000,000)
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| Keywords | skin ulser / wound healing / TNF-alpha / nerve regeneration / 腫瘍壊死因子 / 神経成長因子 / 線維芽細胞 / サイトカイン / シグナル伝達 / MAPキナーゼ |
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| Research Abstract |
The effect of topical TNF-alpha on cutaneous wound repair with special reference to regeneration of periopheral nerve was investigated in C57BL/KsJ (db/db JcL) that spontaneously develop type II Diabetes Melitus. TNF-alpha at doses of 10 and 100ng, 1 and 10 mug and PBS as control were applied to full thickness skin wound (ca 1cm^2) in dorsal skin. The area of wound was measured and skin specimens were obtained periodically.
1) Regression rate of wound was significantly higher on day 11 in mice treated with 100ngTNF-alpha than control. However, wound worsened in mice that received 1 and 10mugTNF-alpha as compaired with control.
2) Wound was more mature on 14 day in mice treated with 100ngTNF-alpha than control, as exemplified by a decrease in number of neutrophils and eosinophiles in the dermal cellular infiltrate.
3) In the TNF-alpha-treated group regeneration of preripheral nerve was apparent on day 14 compared with control, because nerve fibers were occasionally stained with an antineurofilament protein antibody.
These results suggest that TNF-alpha at appropriate concentrations is expected to accelerate cutaneous wound healing, especially in terms of nerve regeneration.
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