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Development of New Anti-diabetic Peptide Drug

Research Project

Project/Area Number 06557052
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionInstitute for Molecular and Cellular Regulation, Gunma University

Principal Investigator

SHIBATA Hiroshi  Department of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Associate Professor, 生体調節研究所, 助教授 (20235584)

Co-Investigator(Kenkyū-buntansha) KOJIMA Itaru  Gunma University, Professor, 生体調節研究所, 教授 (60143492)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywordsinsulin / glucose transporter / G-protein / mastoparan / グルコーストランスポーター / グルコーストランスポート / G蛋白
Research Abstract

Mastoparan, a tetradecapeptide from wasp venom, stimulated glucose uptake in isolated rat adipocytes. The effects of mastoparan were concentration-dependent and the maximal stimulation was achieved at 50muM,which was nearly identical to that induced by insulin. Mas 7, a more potent mastoparan analogue on GTP-binding proteins such as Gi and Go, stimulated glucose transport at lower concentrations than mastoparan. On the other hand, Mas 17, an inactive analogue, was without effect on glucose transport. In the presence of the major histocompatibility complex class I antigen-derived peptide, D^k- (62-85), a potent inhibitor of GLUT4 internalization, the concentration-response relationship of mastoparan effects was markedly shifted to the left without change of the maximal effect. Immunoblot analysis revealed that mastoparan promoted translocation of glucose transporter isoform, GLUT4, from intracellular pool to the plasma membrane. The effects of mastoparan on glucose transport were not inhibited by wortmannin, a specific inhibitor of phosphatidylinositol-3 kinase, but were completely abolished by a phospholipase A2 inhibitor quinacrine. Pertussis toxin pretreatment did not affect mastoparn's effect. ATP-depletion by KCN pretreatment abolished stimulation of glucose transport induced by insulin or GTPgammaS but not by mastoparn. In summary, mastoparan stimulates glucose transport via GTP-binding protein-independent mechanism. Phospholipase A2 may be involved in mastoparan effect on glucose transport.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Shibata H.,他: "Dissection of GLUT4 recycling pathway into exocytosis and endocytosis in rat adipocytes: Evidence that GTP-binding proteins are involved in both processes." J. Biol. Chem.270. 11489-11495 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shibata H.,他: "A synthetic peptide corresponding to the Rab4 hypervariable carboxy-terminal domain inhibits insulin action on glucose transportin rat adipocytes." J. Biol. Chem.271. 9704-9709 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 柴田 宏、河野哲郎: "糖の膜透過に対するインスリン作用の展望.「分子糖尿病学の進歩-基礎から臨床まで-1995」(pp. 69-76)" 金原出版, 250 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shibata, H., Suzuki, Y., Omata, W., Tanaka, S.and Kojima, I.: "Dissection of GLUT4 recycling pathway into exocytosis and endocytosis in rat adipocytes : Evidence that GTP-binding proteins are involved in both processes." J.Biol.Chem.270. 11489-11495 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shibata, H., Omata, W., Suzuki, Y., Tanaka, S.and Kojima, I.: "A synthetic peptide corresponding to the Rab4 hypervariable carboxy-terminal domain inhibits insulin action on glucose transport in rat adipocytes." J.Biol.Chem.271. 9704-9709 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shibata, H.他: "Dissection of GLUT4 recycling pathway into exocytosis and endocytosis in rat adipocytes : Evidence that GTP-binding proteins are involved in both processes." J. Biol. Chem.270. 11489-11495 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shibata, H.他: "A synthetic peptide corresponding to the Rab4 hypervariable carboxy-terminal domain inhibits insulin action on glucose transport in rat adipocytes" J. Biol. Chem.(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shibata,H.,Suzuki,Y.,Omata W.Tanaka,S.,Kojima,I.: "Dissection of GLUT4 recycling pathway into exocytosis and endocytosis in rat adipocytes: Evidence that GTP-binding proteins are involved in both processes." J.BIOL.Chem.(印刷中). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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