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New and rapid diagnostic method for cardiovascular disease and prothrombotic state

Research Project

Project/Area Number 06557063
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

MIYATA Toshiyuki  National Cardiovascular Center Research Institute, Senior Staff, 脈管生理部, 室長 (90183970)

Co-Investigator(Kenkyū-buntansha) USHIZAWA Kouji  Daiichi Chemical Ltd. Chief, 東京研究所, グループ長
YAMAGUCHI Takenori  National Cardiovascular Center Vice President of Hospital, 副病院長
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1995: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordscoagulation factor VII / cardiovascular disease / diabetics / stroke / coronary artery disease / prethrombotic state / thrombosis / diagnosis / 臨床検査法 / 凝固亢進状態 / 第VII因子
Research Abstract

Factor VII (FVII) plays an important role in initiation of the tissue factor-induced coagulation pathway and an increase in FVII coagulant activity (FVIIc) has been proposed as an independent risk factor for coronary artery disease. However, it remains uncertain whether high FVIIc levels are due to an increase in the activation of FVII or due to an increase in the concentration of FVII mass. We developed a new fluorogenic assay for plasma activated FVII (FVIIa) using soluble tissue factor. The sensitivity of this assay ranged from 0.2 to 1,000 ng of FVIIa per milliliter of plasma. Plasma FVIIa levels were measured in 110 healthy subjects and 93 patients with hypertension, diabetes mellitus, and/or cardiovascular disease. The mean plasma FVIIa level in healthy Japanese individuals was 2.5 ng/ml and it was lower than that in Western subjects. Aging increased both the FVIIa level and FVII mass, while menopause mainly increased the FVII mass. Elderly patients with coronary artery disease and/or chronic cerebral infarction showed an increase in the plasma FVIIa levels and the FVIIa/FVIIag ratio. Arterial cardiovascular disease was more common in a high-FVIIa elderly group than in a low-FVIIa elderly group. Plasma FVIIa levels were not correlated with the serum levels of total cholesterol or triglycerides. The FVIIa level and the FVIIa/FVIIag ratio were positively correlated with the fibrinogen level and were negatively correlated with the body mass index and the serum albumin level in the elderly. In conclusion, aging, cardiovascular disease, and malnutrition increased the plasma FVIIa level. FVIIa levels were not correlated with the levels of lipids and the activity of hepatic synthesis, indicating that FVIIa may be an independent risk factor for cardiovascular disease.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] T. Miyata, et al.: "Factor vII hyperactivity and ischaemic heart disease." Lancet. 343. 233 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata, et al.: "Fluorogenic assay of activated factor VII : plasma factor VIIa levels in relation to arterial cardiovascular diseases in Japanese." Arterioscler. Thromb.14. 265-274 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata, et al.: "Suppression of plasma-activated factor VII levels by warfarin therapy." Arterioscler. Thromb. Vasc. Biol.15. 241-246 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata, et al.: "The strong positive correlation between factor VII clotting activity using bovine thromboplastin and the activated factor VII level." Thromb. Haemost.73. 429-434 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata, et al.: "Genetic determinants of plasma factor VII and activated factor VII activity in the Japanese." Thromb. Haemost.73. 617-622 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata, et al.: "Marked increase of activated factor VII in uremic patients." Thromb. Haemost.73. 763-767 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Kario, T. Matsuo, H. Kobayashi, M. Matsuo, T. Sakata, and T. Miyata: "Activation of tissue factor-induced coagulation and endothelial cell dysfunction in non-insulin-dependent diabetic patients with microalbuminuria." Arterioscler. Thromb. Vasc. Biol.15. 1114-1120 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Kario, T. Matsuo, H. Kobayashi, M. Matsuo, T. Sakata, T. Miyata, and K. Shimada: "Factor VII hyperactivity and endothelial cell damage are found in elderly hypertensives only when concomitant with microalbuminuria." Arterioscler. Thromb. Vasc. Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮田敏行: "ビタミンK(医学・生物学領域における新展開)" メディカル・ジャーナル社, 18 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮田敏行、岩永貞昭: "血栓・止血・線溶" 中外医学社, 14 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 宮田敏行: "血液病学、第2版" 文光堂, 11 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Sakata.T.Matsuo, T.Miyata: "Factor VII hyperactivity and ischaemic heart disease." Lancet. 343. 233 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Miyata, T.Sakata, T.Matsuo, H.Kato: "Fluorogenic assay of activated factor VII : plasma factor VIIa levels in relation to arterial cardiovascular diseases in Japanese." Arterioscler. Thromb.14. 265-274 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Sakata, K.Kario, T.Matsuo, Y.Katayama, T.Matsuyama, H.Kato, T.Miyata: "Suppression of plasma-activated factor VII levels by warfarin therapy." Arterioscler.Thromb.Vasc.Biol.15. 241-246 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Matsuo, R.Asada, T.Sakata, H.Kato, T.Miyata: "The strong positive correlation between factor VII clotting activity using bovine thromboplastin and the activated factor VII level." Thromb.Haemost.73. 429-434 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.kario, N.Narita, T.Matsuo, K.Kayaba, A.Tsutsumi, M.Matsuo, T.Miyata, K.Shimada: "Genetic determinants of plasma factor VII and activated factor VII activity in the Japanese." Thromb.Haemost.73. 617-622 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Matsuo, M.Matsuo, M.Koide, T.Yamada, S.Nakamura, T.Sakata, H.Kato, T.Miyata: "Marked increase of activated factor VII in uremic patients." Thromb.Haemost.73. 763-767 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T,Matsuo, H.Kobayashi, T.Sakata, T.Miyata, K.Shimada: "Gender differences of disturbed hemostasis related to fasting insulin level in healthy very elderly Japanese aged *75 years." Atherosclerosis. 116. 211-219 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Matsuo, H.Kobayashi, M.Matsuo, T.Sakata, T.Miyata: "Vasc. Activation of tissue factor-induced coagulation and endothelial cell dysfunction in non-insulin-dependent diabetic patients with microalbuminuria." Arterioscler. Thromb. Biol.15. 1114-1120 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Kario, T.Matsuo, H.Kobayashi, M.Matsuo, T.Sakata, T.Miyata, K.Shimada: "Factor VII hyperactivity and endothelial cell damage are found in elderly hypertensives only when concomitant with microalbuminuria." Arterioscler. Thromb. Vasc. Biol.in press.(1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Miyata,et al.: "Factor VII hyperactivity and ischaemic heart disease." Lancet. 343. 233 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miyata,et al.: "Fluorogenic assay of activated factor VII: plasma factor VIIa levels in relation to arterial cardiovascular diseases in Japanese." Arterioscler. Thromb.14. 265-274 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miyata,et al.: "Suppression of plasma-activated factor VII levels by warfarin therapy." Arterioscler. Thromb. Vasc. Biol.15. 241-246 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miyata,et al.: "The strong positive correlation between factor VII clotting activity using bovine thromboplastin and the activated factor VII level." Thromb. Haemost.73. 429-434 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miyata,et al.: "Genetic determinants of plasma factor VII and activated factor VII activity in the Japanese." Thromb. Haemost.73. 617-622 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Miyata,et al.: "Marked increase of activated factor VII in uremic patients." Thromb. Haemost.73. 763-767 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宮田敏行: "ビタミンK(医学・生物学領域における新展開)" メディカル・ジャーナル社, 18 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 宮田敏行、岩永貞昭: "血栓・止血・線溶" 中外医学社, 14 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Kario ら: "Factor VII in non-insulin-dependent diabetic patients with microalbuminuria." Lancet. 342. 1552- (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Kario ら: "Factor VII hyperactivity and ischaemic heart disease." Lancet. 343. 233- (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Miyata ら: "Three missense mutations in the protein C heavy chain causing type I and type II protein C deficiency." Thromb,Haemost. 71. 32-37 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Kario ら: "Fluorogenic assay of activated factor VII:plasma factor VIIa levels in relation to arterial cardiovascular disease in Japanese." Arterioscler Thromb,. 14. 265-274 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Miyata ら: "Factor IX Bm Kiryu:A Val-313-to-Asp substitution in the catalytic domain results in loss of function due to a conformational change of…" Br.I.Haematal,. 88. 156-165 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Z.Zheng ら: "Six missense mutations associated with type I and type II protein C deficiency and implications obtained from molecular modeling." Blood Coagul,Fibrinolysis. 5. 687-696 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 宮田敏行: "「ビタミンK(医学・生物学領域における新展開)」V.ビタミンK依存性蛋白質の構造とその機能、A.プロトロンファミリー、2)VII因子" メディカル ジャーナル社, 18 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 宮田敏行、岩永貞昭: "「血栓・止血・線溶」、III.血液凝固とその制御、1.血液凝固機序、b.凝固因子の構造と機能、3)第VII因子・組織因子・リン脂質" 中外医学社, 14 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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