• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The development of a new diagnostic method for uterine endometrial cancer, using monoclonal antibody for glicolipids, and its application for mass screening

Research Project

Project/Area Number 06557088
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionKeio University

Principal Investigator

NOZAWA Shiro  Keio Univ.Sch.of Med., Professor, 医学部, 教授 (90051557)

Co-Investigator(Kenkyū-buntansha) SUGIHARA Keisuke  Mochida Pharmaceutical CO., LTD,Manager, バイオサイエンス研究所, 試薬マネージャー
AOKI Daisuke  Keio Univ.Sch.of Med., Instructor, 医学部, 助手 (30167788)
TSUKAZAKI Katsumi  Keio Univ.Sch.of Med., Assistant Professor, 医学部, 講師 (40118972)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1995: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1994: ¥3,900,000 (Direct Cost: ¥3,900,000)
Keywordsendometrial cancer / monoclonal antibody MSN-1 / enzyme immunoassay
Research Abstract

We measured the levels of the "MSN-1" antigen (mainly the Lewis^b sugar chain) in endometrial cell samples using an enzyme immunoassay (EIA) with the anti-uterine cancer monoclonal antibody MSN-1. We developed a new diagnostic EmC-EIA (Endometrial Cell Enzyme Immunoassay) method for endometrial cancer which enabled distinction between normal endometrial cells and cancer cells according to quantitative differences in the level of MSN-1 antigen.The original EmC-EIA method was also improved. Since long term storage of samples is possible and the procedure is simple, this EmC sandwich EIA method could easily be made into a kit. Its clinical applicability was confirmed using many clinical samples. The EmC sandwich EIA method involves solubilization with surfactant of the residual cells adhering to the sample collection device after endometrial cytodiagnosis. The primary antibodies for actin (protein content) and MSN-1 are bound to a microtiter plate. Subsequently, the amount of MSN-1 antige … More n per unit protein in the cell sample is measured by a sandwich EIA using enzme-labeled MSN-1 and antiactin antibodies. We investigated 48 samples of normal endometrium, 11 of endometrial hyperplasia, and 45 of endometrial cancer. The positive rate (when the mean +2 SD for normal endometrium was taken as the cut-off value) was 4.2% for normal endometrium, 45.5% for endometrial hyperplasia, and 66.7% for endometrial cancer. The positive rate was 76% for well-differentiated endometrial adenocarcinoma, a particularly high value. In a study of 35 patients with uterine cancer who underwent both cytodiagnosis and the EmC sandwich EIA,the positive rate was 63.3% for cytodiagnosis and 63.3% for the EmC sandwich EIA.However, when the two methods were combined, the positive rate rase 83.3%.
In conclusion, the EmC sandwich EIA was found to be useful as a auxiliary procedure to cytodiagnosis in patients with uterine cancer, especially those with well-differentiated adenocarcinoma which is often hard to evaluate by cytodiagnosis. Since this assay has various features such as application as a kit, it could also be used for screenig. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kaneyuki Kubushiro: "Enzymatic basis for the accumulation of Lewis^b antigen in uterine endometrial cancer." Japanese Journal of Cancer Research. 86. 361-367 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 高松潔: "子宮癌と腫瘍マーカー" 産婦人科治療. 70. 405-411 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久布白兼行: "子宮体癌update糖鎖抗原との関連" 産科と婦人科. 62. 803-809 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 久布白兼行: "子宮体癌細胞におけるフコシル化糖鎖の組織接着ならびに転移に与える影響" 腫瘍マーカー研究会誌. 10. 183-185 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 福地剛: "子宮体癌補助診断法(EmC-EIA法)における新たな測定系(Sandowich法の)開発の試み" 腫瘍マーカー研究会誌. 10. 247-249 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 塚崎克己: "子宮体癌における複合糖質の変化" 病理と臨床. 14. 221-229 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al: "Expression mechanism of human uterine endometrial cancer-specific fucosylated carbohydrate chains, aberrant alpha1*4 fucosyltransferase in uterine endometrial cancer-derived cell lines with type I carbohydrate chain" International Journal of Oncology. 6. 93-97 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Biological effects of MSN-1 recognized antigen on tissue adhesion and metastasis in endometrial cancer" Acta Obst Gynaec Jpn. 47 (2). 163-164 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Eyzymatic basis for the accumulation of Lewis^b antigen in uterine endometrial cancer" Japanese Journal of Cancer Research. 86. 361-367 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Abnormal expressin of carbohydrate chain and its mechanism in endometrial cancer." Nihonrinsho. 53 (7). 197-203 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kaneyuki Kubushiro, et al.: "Effect of fucosylated carbohydrate chain on tissue adhesiveness and metastasis in uterine endometrial cancer" Tumor Marker Research. 10. 183-185 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 塚崎克己: "婦人科がん検診の現況と展望 子宮体癌検診" 産科と婦人科. 61. 947-954 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久布白兼行: "子宮体癌における複合糖質の発現異常とその浸潤・転移に及ぼす影響" 臨床婦人科産科. 49. 225-239 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneyuki Kubushiro: "Expression mechanism of human uterine endometrial cancer-specific fucosylated carbohydrate chains : aberrant α1→4 fucosyltransferase in uterine endometrial cancer-derived cell lines with type I carbohydrate chain" International Journal of Oncology. 6. 93-97 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 久布白兼行: "子宮体癌におけるMSN-1認識抗原の組織接着,ならびに転移に与える影響" 日本産科婦人科学会雑誌. 47. 163-164 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneyuki Kubushiro: "Enzymatic basis for the accumulation of Lewis^b antigen in uterine endometrial cancer" Japanese Journal of Cancer Research. 86. 361-367 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 塚崎克己: "子宮体癌における複合糖質の変化" 病理と臨床. 14. 221-229 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 塚崎 克己: "抗子宮体癌モノクローナル抗体MSN-1の特性とその臨床応用への試み" 癌と化学療法. 21. 141-149 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 塚崎 克己: "婦人科がん検診の現況と展望 子宮体癌検診" 産科と婦人科. 61. 947-954 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 高松 潔: "腫瘍マーカーの現状と展望" 産婦人科の実際. 44. 41-47 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 久布白兼行: "子宮体癌における複合糖質の発現異常とその浸潤・転移に及ぼす影響" 臨床婦人科産科. 49. 225-239 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kaneyuki Kubushiro: "Expression mechanism of human uterine endometrial cancer-specific fucosylated carbohydrate chains:aberrant α 1→4 fucosyltransferase in uterine endometrial cancer-derived cell lines with type I carbohydrate chain." International Journal of Oncology. 6. 93-97 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 久布白兼行: "子宮体癌におけるMSN-1認識抗原の組織接着,ならびに転移に与える影響" 日本産科婦人科学会雑誌. 47. 163-164 (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi