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DEVELOPMENT OF A NEW CLINICAL PARAMETER FOR ASSESSMENT OF PROGRESSIVE PERIODONTAL DIDEASE AND ITS THERAPEUTIC AND PREVENTIVE DRUGS

Research Project

Project/Area Number 06557098
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Functional basic dentistry
Research InstitutionKYUShU UNIVERSITY

Principal Investigator

YAMAMOTO Kenji  KYUSHU UNIVERSITY,DENTISTRY,PROFESSOR, 歯学部, 教授 (40091326)

Co-Investigator(Kenkyū-buntansha) NAKANISHI Hiroshi  KYUSHU UNIVERSITY,DENTISTRY,Research Associate, 歯学部, 助手 (20155774)
KUNIMATSU Kazushi  NAGASAKI UNIVERSITY,DENTISTRY,ASSISTANT PROFESSOR, 歯学部, 講師 (20170011)
金井 貞  サントリー株式会社, 生物医学研究所, 所長
赤峰 昭文  九州大学, 歯学部, 教授 (00117053)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1995: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1994: ¥11,000,000 (Direct Cost: ¥11,000,000)
Keywordsperiodontal disease / clinical parameter / drug design / proteinases / Porphyromonas gingivalis / oral bacterium / cathepsins / Arg-gingipain / 臨床評価法の確立 / システインプロテアーゼ / 歯肉溝滲出液 / 病原性プロテアーゼ
Research Abstract

Destruction of periodontal tissues, including alveolar bone, is one of the most important pathological conditions of progressive periodontal disease. Although the actual mechanism of periodontal tissue breakdown during disease progression remains to be clarified, proteolytic enzymes derived from both host cells and oral microorganisms are thought to play a key role in pathogenicity of the disease. The present study was undertaken to establish new clinical paramethers for diagnosis of the disease and to develop new therapeutic and prevenmtive drugs. For this purpose, the present work was designed to identify periodontopathogenic proteinases and to clarify how they participate in the progression of the disease. Host-derived proteinases, including cysteine proteinases (cathepsin B,H,and L), serine proteinases (cathepsin G and medullasin) and aspartic proteinase (cathepsin D), were released into gingival crevices of periodontitis patients and their levels in gingival crevicular fluid (GCF) were positively correlated with the severity of the disease. A major cysteine proteinases produced by the major periodontopathogen Porphyromonas gingivalis, termed Arg-gingipain, was also concentrated in GCF of the patients. The enzyme was shown to be involved in the direct destruction of periodontal tissues and the disrukption of normal host defense mechanisms. The enzyme was also implicated in the hemagglutinating activity of the organism.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (100 results)

All Other

All Publications (100 results)

  • [Publications] Tetsuya Goto et al.: "Localization of cathepsins B,D,and L in rat osteoclasts by immuno-light and -electron microscopy" Histochemistry. 101. 33-40 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masayuki Yamada et al.: "Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methyl-otrophic yeast,Pichia pastoris and characterization of produced recombinant cathepsin E" Biochim.Biophys.Acta. 1206. 279-285 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Nakanishi et al.: "Age-related changes in activities and localizations of cathepsins D,E,B,and L in the rat brain tissues." Exp.Neurol.126. 119-128 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomoko Kadoawki et al.: "Purification and characterization of a novel arginine-specific cysteine proteinase (argingipain) in volved in the pathogenesis of periodontal disease from the culture supernatant of Porphyromonas gingivalis." J.Biol.Chem.269. 21371-21378 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomomi Shindo et al.: "Calcium-dependent hyperexcitability of hippocampal CAl pyramidal cells in an in vitro slice after ethanol withdrawal of the rat." Brain Res.656. 432-436 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamotsu Kiyoshima et al.: "Immunocytochemical localization of cathepsin L in the synovial lining cells of the rat temporomandibular joint." Arch.oral Biol.39. 1049-1056 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 中西博 他: "神経細胞死と細胞内プロテアーゼ" 日本薬理学雑誌. 105. 1-9 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazushi Kunimatsu et al.: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects." J.Periodont.Res.30. 51-57 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kuniaki Okamoto et al.: "Structural characterization of argingipain. a novel arginine-specific cysteine proteinase as a major periodontal pathogenic factor from Porphyromonas gingivalis." Arch. Biochem.Biophys.316. 917-925 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本健二: "骨吸収機構における破骨細胞リソソーム蛋白質の役割" 福岡医学雑誌. 85. 133-137 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama K.,et al.: "Construction and characterization of arginine-specific cysteine proteinase (Arg-gingipain)-deficient mutants of Porphyromonas gingivalis. Evidence for significant contribution of Arg-gingipain to virulence." J.Biol.Chem.270. 23619-23626 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K.,et al.: "Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E" J.Periodont.Res.30. 51-57 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K.,et al.: "Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E" Arch.Biochem.Biophys.322. 103-111 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimine Y.et al.: "Specific immunocytochemical localization of cathepsin E at the ruffled border membrane of active osteoclasts" Cell Tissue Res.281. 85-91 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本健二: "歯周病原性プロテアーゼの機能と病態" 日本薬理学雑誌. 105. 345-355 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsukuba T.,et al.: "Biochemical properties of the monomeric mutant of human cathepsin E expressed in Chinese hamster ovary cells : Comparison with dimeric forms of the natural and recombinant cathepsin E." J.Biochem.119. 126-134 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuo K.,et al.: "Immunohistochemical localization of cathepsins D and E in human gastric cancer. A possible correlation with local invasive and metastatic activities of carcinoma cells." Human Pathol.27. 184-190 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kouzuma Y.,et al.: "Purification,characterization,and sequencing of two cysteine proteinase inhibitors,Sca and Scb,from Sunflower (Helianthus annuus)." J.Biochem.119. 1106-1113 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama K.,et al.: "Involvement of arginine-specific cyeteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis." J.Bacteriol.178. 2818-2824 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma T.,et al.: "Expression of cathepsin E in pancreas : A possible tumor marker for pancreas,a preliminary report." Int.J.Cancer. 67. 492-497 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K.,et al.: "Cloning and sequencing of the gene encoding a novel lysine-specific cysteine proteinase (Lys-gingipain) in Porphyromonas gingivalis : Structural relationship with arginine-specific cysteine proteinase (Arg-gingipain)." J.Biochem.120. 398-406 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishishita K.,et al.: "Age-related and dexamethasone-induced changes in cathepsins E and D in rat thymic and splenic cells." Arch.Biochem.Biophys.333. 349-358 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi H.,et al.: "Increased expression of cathepsins E and D in neurons of the aged rat barin and their colocalization with lipofuscin and carboxy-terminal fragmnets of Alzheimer amyloid precursor protein." J.Neurochem.68. 739-749 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kunimatsu K.,et al.: "Immunohistochemical study of cathepsin G and medullasin in inflamed gingival tissues from periodontal patients." J.Periodont.Res.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kenji Yamamoto et al.: "Biological Functions of Proteases and Inhibitors" Japan Scientific Societies Press, 274 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kenji Yamamoto et al.: "Pharmacological Approach to the Study of the Formation and the Mechanisms of Hard Tissues." Ishiyaku EuroAmerica Inc., 186 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroshi Nakanishi et al.: "The SAM Model of Senescence" Elsevier Science B.V., 458 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本健二: "化学と生物" 学会出版センター, 67 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto K.,et al.: "Aspartic Proteinases : Structure,Function,Biology,Biomedical Implication" Plenum Press (New York), 223-229 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsukuba T.,et al.: "Aspartic Proteinases : Structure,Function,Biology,Biomedical Implication" Plenum Press (New York), 331-334 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto K.,et al.: "Intracellular Protein Catabolism" Plenum Press (New York), 306 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山本健二 他: "Moderm Physician" 振興出版社, 24 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto K.,et al.: "Medical Aspects of Proteases and Protease Inhibitors" IOS Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamamoto K.,et al.: "Proteolysis and Protein Turnover" IOS Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsukuba T.et al.: "Medical Aspects of Proteases and Protease Inhibitors" IOS Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sakai H.,et al.: "Proteolysis and Protein Turnover" IOS Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K.,et al.: "Proteolysis and Protein Turnover" IOS Press (in press), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kadowaki T., Yoneda, M., Okamoto K., Maeda K., and Yamamoto K.: "Purification and characterization of a novel arginine-specific cysteine proteinase (argingipain) involved in the pathogenesis of periodontal disease from the culture supernatants of Porphyromonas gingivalis." J.Biol.Chem.269, No.33. 21371-21378 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kiyoshima T., Kido M.A., Nishimura Y., Himeno M., Tsukuba T., Yamamoto K., and Tanaka T.: "Immunocytochemical localization of cathepsin L in the synovial lining cells of the rat temporomandibular joint." Archs.oral Biol.39. 1049-1056 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kiyoshima T., Kido, A.M., Tsukuba T., Sakai H., Yamamoto K., and Tanaka T.: "Localization of cathepsins B and D in the synovial lining cells of the normal rat temporomadibular joint by immuno-light and -clectron microscopy." Acta Histochem.Cytochem. 27. 441-450 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi H., Tominaga K., Amano T., Hirotsu I., Inoue T., and Yamamoto K.: "Age-related changes in activities and localizations of cathepsins D,E,B,and L in the rat brain tissues." Exp.Neurol.126, No.1. 119-128 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Goto T., Kiyoshima T., Moroi R., Tsukuba T., Nishimura Y., Himeno M., Yamamoto K., and Tanaka T.: "Localization of cathepsin B,D,and L in rat osteoclasts by immuno-light and -electron microscopy." Histochemistry. 101, No.1. 33-40

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamada M., Azuma T., Matsuba T., Iida H., Suzuki H., Yamamoto K., Kohli Y., and Hori H.: "Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast, Pichia pastris and characterization of produced recombinant cathepsin E." Biochim.Biophys.Acta.1206. 279-285 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shindo T., Watanabe S., Kamata O., Yamamoto K., and Nakanishi H.: "Calcium-dependent hyperexcitability of hippocampal CA1 pyramidal cells in an in vitro slice after ethanol withdrawal of the rat." Brain Res.656. 432-436 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kunimatsu K., Mine N., Muraoka Y., Kato I., Hase T., Aoki Y., and Yamamoto K.: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects." J.Periodont.Res.30. 51-57 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K., Misumi Y., Kadowaki T., Yoneda M., Yamamoto K., and Ikehara Y.: "Structural characterization of argingipain, a novel arginine-specific cysteine proteinase as a major periodontal pathogenic factor from Porphyromonas gingivalis." Arch.Biochem.Biophys.316. 917-925 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshimine Y., Tsukuba T., Isobe R., Sumi M., Akamine A., Maeda K., and Yamamoto K.: "Specific immunocytochemical localization of cathepsin E at the ruffled border membrane of active osteoclasts." Cell Tissue Res.281. 85-91 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama K., Kadowaki T., Okamoto K., and Yamamoto K.: "Construction and charactrization of arginine-specific cysteine proteinase (Arg-gingipain) -deficient mutants of Porphyromonas gingivalis. Evidence for significant contribution of Arg-gingipain to virulence." J.Biol.Chem.270, No.40. 23619-23626 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano T., Nakanishi H., Kondo T., Tanaka T., Oka M., and Yamamoto K.: "Age-related changes in cellular localization and enzymatic activities of cathepsins B,L and D in the rat trigeminal ganglion neuron." Mech.Ageing Dev.83. 133-141 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Amano T., Nakanishi H., Oka M., and Yamamoto K.: "Increased expression of cathepsins E and D in reactive microglial cells associated with spongiform degeneration in the brain stem of senescence-accelerated mouse." Exp.Neurol.136. 171-182 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okada M., Nakanishi H., Tamura A., Urae A., Mine K., Yamamoto K., and Fujiwara M.: "Long-term spatial cognitive impairment after middle cerebral artery occlusion in rats. No involvement of the hippocampus." J.Cereb.Blood Flow Met.15. 1012-1021 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsukuba T., Sakai H., Yamada M., Maeda H., Hori H., Azuma T., Akamine A., and Yamamoto K.: "Biochemical properties of the monomeric mutant of human cathepsin E expressed in Chinese hamster ovary cells. Comparison with dimeric forms of the natural and recombinant cathepsin E." J.Biochem.119. 126-134 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakayama K., Yoshimura F., Kadowaki T., and Yamamoto K.: "Involvement of arginine-specific cysteine proteinase (Arg-gingipain) in fimbriation of Porphyromonas gingivalis." J.Bacteriol.178, No.10. 2818-2824 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kouzuma Y., Kawano K., Kimura M., Yamasaki N., Kadowaki T., and Yamamoto K.: "Purification, characterization, and sequencing of two cysteine proteinase inhibitors, Sca and Scb, from the sunflower (Helianthus annuus) seeds." J.Biochem.119, No.6. 1106-1113 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto K., Kadowaki T., Nakayama K., and Yamamoto K.: "Cloning and sequencing of the gene encoding a novel lysine-specific cysteine proteinase (Lys-gingipain) in Porphyromonas gingivalis : Structural relationship with arginine -specific cysteine proteinase (Arg-gingipain)." J.Biochem.120. 135-137 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuo, K., Kobayashi I., Tsukuba T., Kiyoshima T., Ishibashi Y., Miyoshi A., Yamamoto K., and Sakai H.: "Immunohistochemical localization of cathepsins D and E in human gastric cancer. A possible correlation with local invasive and metastatic activities of carcinoma cellos." Human pathol.27. 184-190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Azuma T., Hirai M., Ito S., Yamamoto K., Taggart R.T., Matsuba T., Yasukawa K., Uno K., Hayakumo T., and Nakajima M.: "Expression of cathepsin E in pancreas : A possible tumor marker for pancreas, a prelininary report." Int.J.Cancer. 67. 492-497 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishishita K., Sakai H., Sakai E., Kato Y., and Yamamoto K.: "Age-related and dexamethasone-induced changes in cathepsins E and D in rat thymic and splenic cells." Arch.Biochem.Biophys.333, No.2. 349-358 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi H., Kawachi A., Okada M., Fujiwara M., and Yamamoto K.: "Protective effect of MK-801 on the anoxia-aglycemia induced damage in the fluorocitrate-treated hippocampal slice of the rat" Brain Res.732. 232-236 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi H., Amano T., Sastradipura D.F., Yoshimine Y., Tsukuba T., Tanabe K., Hirotsu I., Ohono T., and Yamamoto K.: "Increased expression of cathepsins E and D in neurons of the aged rat brain and their colocalization with lipofuscin and carboxy-terminal fragments of Alzheimer amyloid precursor protein." J.Neurochem.68, No.2. 739-749 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakanishi H., Tamura A., Kawai K., and Yamamoto K.: "Electrophysiological studies of rat substantia nigra neurons in an in vitro slice preparation after middle cerebral artery occulusion." Neuroscience. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kunimatsu K., Ozaki Y., Hara Y., Aoki Y., Yamamoto K., and Kato I.: "Immunohistochemical study of cathepsin G and medullasin in inflamed gingival tissues from periodontal patients." J.Periodont.Res.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsukuba T.,et al.: "Biochemical properties of the monomeric mutant of human cathepsin E expressed in Chinese hamster ovary cells:Comparison with dimeric forms of the natural and recombinant cathepsin E." J.Biochem.119. 126-134 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuo K.,et al.: "Immunohistochemical localization of cathepsins D and E in human gastric cancer.A possible correlation with local invasive and metastatic activities of carcinoma cells." Human Pathol.27. 184-190 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kouzuma Y.,et al.: "Purification,characterization,and sequencing of two cysteine proteinase inhibitors,Sca and Scb,from Sunflower(Helianthus annuus)." J.Biochem.119. 1106-1113 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakayama K.,et al.: "Involvement of arginine-specific cyeteine proteinase(Arg-gingipain)in fimbriation of Porphyromonas gingivalis." J.Bacteriol.178. 2818-2824 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Azuma T.,et al.: "Expression of cathepsin E in pancreas:A possible tumor marker for pancreas,a preliminary report." Int.J.Cancer. 67. 492-497 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okamoto K.,et al.: "Cloning and sequencing of the gene encoding a novel lysine-specific cysteineProteinase(Lys-gingipain)in porphyromonas gingivalis:Structural relationship with arginine-specific cysteine proteinase(Arg-gingipain)." J.Biochem.120. 398-406 (1996)

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      1996 Annual Research Report
  • [Publications] Nishishita K.,et al.: "Age-related and dexamethasone-induced changes in cathepsins E and D in rat thymic and splenic cells." Arch.Biochem.Biophys.333. 349-358 (1996)

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      1996 Annual Research Report
  • [Publications] Nakanishi H.,et al: "Increased expression of cathepsins E and D in neurons of the aged rat barin and their colocalization with lipofuscin and carboxy-terminal fragmnets of Alzheimer amyloid precursor protein." J.Neurochem.68. 739-749 (1997)

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      1996 Annual Research Report
  • [Publications] Kunimatsu K.,et al.: "Immunohistochemical study of cathepsin G and medullasin in inflamed gingival tissues from periodontal patients." J.Periodont.Res.(in press). (1997)

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      1996 Annual Research Report
  • [Publications] Yamamoto K.,et al.: "Intracellular Protein Catabolism" Plenum Press(New York), 306 (1996)

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      1996 Annual Research Report
  • [Publications] 山本健二 他: "Modem Physician" 新興出版社, 24 (1996)

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      1996 Annual Research Report
  • [Publications] Yamamoto K.,et al.: "Medical Aspects of Proteases and Protease Inhibitors" IOS Press(in press), (1997)

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      1996 Annual Research Report
  • [Publications] Yamamoto K.,et al.: "Proteolysis and Protein Turnover" IOS Press(in press), (1997)

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      1996 Annual Research Report
  • [Publications] Tsukuba T.: "Medical Aspects of Proteases and Protease Inhibitors" IOS Press(in press), (1997)

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      1996 Annual Research Report
  • [Publications] Sakai H.,et al.: "Proteolysis and Protein Turnover" IOS Press(in press), (1997)

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      1996 Annual Research Report
  • [Publications] Okamoto K.,et al: "Proteolysis and Protein Turnover" IOS Press(in press), (1997)

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  • [Publications] Kunimatsu K.,et al.: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects" J.Periodont.Res.30. 51-57 (1995)

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      1995 Annual Research Report
  • [Publications] Okamoto K.,et al.: "Structural characterization of argingipain,a novel arginine-specific cysteine proteinase as a major periodontal pathogenic factor from Porphyromonas gingivalis" Arch.Biochem.Biophys.316. 917-925 (1995)

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  • [Publications] Nakayama K.,et al.: "Construction and characterization of arginine-specific cysteine proteinase(Arg-gingipain)-deficient mutants of Porphyromonas gingivalis.Evidence for significant contribution of Arg-gingipain to virulence." J.Biol.Chem.270. 23619-23626 (1995)

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  • [Publications] Okamoto K.,et al.: "Isolation and sequencing of two cDNA clones encoding rat spleen cathepsin E and analysis of the activation of purified procathepsin E" Arch.Biochem.Biophys.322. 103-111 (1995)

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  • [Publications] Yoshimine Y.,et al.: "Specific immunocytochemical localization of cathepsin E at the ruffled border membrane of active osteoclasts" Cell Tissue Res.281. 85-91 (1995)

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  • [Publications] 山本健二: "歯周病原性プロテアーゼの機能と病態" 日本薬理学雑誌. 105. 345-355 (1995)

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  • [Publications] Yamamoto K.: "Aspartic Proteinases:Structure,Function,Biology,Biomedical Implication" Plenum Press(New York), 223-229 (1995)

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  • [Publications] Tsukuba T.,et al.: "Aspartic Proteinases:Stucture,Function,Biology,Biomedical Implication" Plenum Press(New York), 331-334 (1995)

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  • [Publications] Tomomi Shindo: "Calcium-dependent hyperexcitability of hippocampal CA1 pyramidal cells in an in vitro slice after ethanol withdrawal of the rat." Brain Res.656. 432-436 (1994)

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  • [Publications] Tamotsu Kiyoshima: "Immunocytochemical localization of cathepsin L in the synovial lining cells of the rat temporomandibular joint." Arch.oral Biol.39. 1049-1056 (1994)

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  • [Publications] Kazushi Kunimatsu: "Identification and possible function of cathepsin G in gingival crevicular fluid from chronic adult periodontitis patients and from experimental gingivitis subjects." J.Periodont.Res.30. 51-57 (1995)

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      1994 Annual Research Report
  • [Publications] Kuniaki Okamoto: "Structural characterization of argingipain.a novel arginine-specific cysteine proteinase as a major periodontal pathogenic factor from Porphyromonas gingivalis." Arch.Biochem.Biophys.316. 917-925 (1995)

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  • [Publications] 山本健二: "骨吸収機構における破骨細胞リソソーム蛋白質の役割" 福岡医学雑誌. 85. 133-137 (1995)

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  • [Publications] 中西博: "神経細胞死と細胞内プロテアーゼ" 日本薬理学雑誌. 105. 1-9 (1994)

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  • [Publications] Tetsuya Goto: "Localization of cathepsins B,D,and L in rat osteoclasts by immuno-light and -electron microscopy" Histochemistry. 101. 33-40 (1994)

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  • [Publications] Masayuki Yamada: "Secretion of human intracellular aspartic proteinase cathepsin E expressed in the methylotrophic yeast,Pichia pastoris and characterization of produced recombinant cathepsin E" Biochim.Biophys.Acta. 1206. 279-285 (1994)

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  • [Publications] Hiroshi Nakanishi: "Age-related changes in activities and localizations of cathepsins D,E,B,and L in the rat brain tissues." Exp.Neurol.126. 119-128 (1994)

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      1994 Annual Research Report
  • [Publications] Tomoko Kadowaki: "Purification and characterization of a novel arginine-specific cysteine proteinase (argingipain) in volved in the pathogenesis of periodontal disease from the culture supernatant of Porphyromonas gingivalis." J.Biol.Chem.269. 21371-21378 (1994)

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  • [Publications] Kenji Yamamoto: "Biological Functions of Proteases and Inhibitors" Japan Scientific Societies Press, 274 (1994)

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      1994 Annual Research Report
  • [Publications] Kenji Yamamoto: "Pharmacological Approach to the Study of the Formation and the Mechanisms of Hard Tissues." Ishiyaku EuroAmerica Inc., 186 (1994)

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  • [Publications] Hiroshi Nakanishi: "The SAM Model of Senescence" Elsevier Science B.V., 458 (1994)

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  • [Publications] 山本健二: "化学と生物" 学会出版センター, 67 (1994)

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Published: 1994-04-01   Modified: 2016-04-21  

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