| Project/Area Number |
06557108
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | Faculty of Dentistry, Tokyo Medical and Dental University |
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Principal Investigator |
AMAGASA Teruo Tokyo Medical and Dental University, Facl.Dent., 1st Dept.Oral Surg.Prof., 歯学部, 教授 (00014332)
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| Co-Investigator(Kenkyū-buntansha) |
OIDA Shin-ichiro Tokyo Medical and Dental University, Facl.Dent., Biochemistry Ass.Prof., 歯学部, 助手 (10114745)
高木 亨 東京医科歯科大学, 歯学部, 講師 (20124696)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥10,900,000 (Direct Cost: ¥10,900,000)
Fiscal Year 1995: ¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 1994: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | AMF / Squamous cell carcinoma / Boyden chamber / Northern blot analysis / RT-PCR / TNM classification / AMFR / invasion and metastasis / ボイデン・チェンバー / 自己分泌型遊走因子 / 分子生物学 / クローニング / レセプター / 細胞工学 |
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| Research Abstract |
AMF (Autocrine Motility Factor) has been reported to be produced by malignant melanoma, fibrosarcoma and bladder carcinoma cells, with highly invasive and metastatic potentials. Boyden chamber analyzes showed that conditioned medium of oral squamous cell carcinoma (SCC) LMF4 cells also induced cell motility in an autocrine fashion. This motile activity was purified by using molecular sieve column chromatography and DEAE high performance liquid chromatography, and was identified as a single protein (LMF4 AMF) with molecular weight of 55kD and 65kD under non-reduced and reduced condition, respectivetly. The LMF4-AMF also induced fibrosarcoma cell motility in a dose-dependent manner as well as other SCC cells. These results suggested that LMF4-AMF was identical to other AMFs reported previously.
The LMF4 cell motility was also induced by the AMF produced by fibrosarcoma cells, suggesting that LMF4 cells expressed functional AMFR (AMF Receptor). Northern blot and RT-PCR analyzes of SCC cells showed that invasive and metastatic cells expressed higher amount of AMFR than non invasive SCC cells. RT-PCR analyzes of clinical specimens showed that prinary cells expressed AMFR in relation to N stages rater than T in TNM classification and that metastasized cells expressed AMFR more than primary.
We conclude that SCC also produced AMF and express AMFR and that these molecules are related to SCC cell motility in association with invasive and metastatic potentials of SCC cells.
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