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Study for both the establishment of in vivo model system for the development of salivary mucous cyst and its treatment

Research Project

Project/Area Number 06557112
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionThe University of Tokushima

Principal Investigator

AZUMA Masayuki  School of Dentistry, Second Department of Oral and Maxillofacial Surgery, University of Tokushima Assistant Professor, 歯学部・附属病院, 講師 (20144983)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Hideo  School of Dentistry, Second Department of Oral and Maxillofacial Surgery, Univer, 歯学部, 助教授 (30116131)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1995: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1994: ¥6,800,000 (Direct Cost: ¥6,800,000)
KeywordsHuman salivary gland duct cells / Cyst / TGF-beta1 / MMP-2
Research Abstract

We have examined the content of matrix metalloproteinase-2 and -9 (MMP-2, MMP-9) in luminal fluid of extravasation mucoceles. The luminal fluid showed a high level of MMP activity compared with saliva from Wharton's duct. This result suggests that proteolytic enzymes are involved in the pathogenesis of mucoceles. Thus, based on the above observation, we attempted to construct a cyst-like structure in vivo, and analyzed molecular mechanisms in the development of the lesion. When SV40-immortalized normal human salivary gland cells (NS-SV-DC) were treated with TGF-beta1 at the concentration of 1 ng/ml or 5 ng/ml followed by co-inoculation with Matrigel into the backs of nude mice, they formed large cystic structures containing luminal fluid. Analysis of luminal fluids contained in cystic cavity demonstrated a high MMP activity. Northern blot analysis indicated that expression of TGF-beta1 and MMP-2 mRNAs in cells was greatly enhanced by treatment with TGF-beta1. Furthermore, the development of cyst formation was almost completely inhibited by the addition of TIMP-1 into Matrigel. These findings, therefore, suggest that the in vivo cyst formation by TGF-beta1-treated cells is associated with the continuous induction of MMP-2 activity.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Masayuki Azuma: "Enhanced proteolytic activity is responsible for the aberrant morphogenetic development of SV40-immortalized normal human salivary gland cells grown on basement membrane components" Lab.Invest.70. 217-227 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 東 雅之: "SV40不死化正常ヒト唾液腺細胞における野生型p53の発現" 日本口腔科学会雑誌. 43. 580-585 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Proteolytic enzymes in salivary extravasation mucoceles" J.Oral Pathol.Med.24. 299-302 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Lack of expression of transforming growth factor-β type II receptor associated with malignant progression in human salivary gland cell clones" Int.J.Cancer. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Different signal pathways involved in transforming growth factor-β1-induced morphologic change and type IV collagen synthesis in SV40-immortalized normal human salivary gland duct and myoeaithlial cell clone" Arch.Oral Biol.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Increased matrix metalloproteinase-2 activity induced by TGF-β1 in human salivary gland duct cells is associated with the development of cyst formation in vivo" J.Oral Pathol.Med.(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Enhanced proteolytic activity is responsible for the aberrant morphogenetic development of SV40-immortalized normal human salivary gland cells grown on basement membrane components." Lab.Invest.70. 217-227 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Expression of wild-type p53 in SV40-immortalized normal human salivary gland cells." J.Jpn.Stomatol.Soc.43. 580-585 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Proteolytic enzymes in salivary extravasation mucoceles." J.Oral Pathol.Med.24. 299-302 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Lack of expression of transforming growth factor-beta type II receptor associated with malignant progression in human salivary gland cell clones." Int.J.Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Different signaling pathways involved in transforming growth factor-beta1-induced morphologic change and type IV collagen synthesis in SV40-immortalized normal human salivary gland duct and myoepithelial cell clones" Arch.Oral Biol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Increased matrix metalloproteinase-2 activity induced by TGF-beta1 in human salivary gland duct cells is associated with the development of cyst formation in vivo." J.Oral Pathol.Med.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masayuki Azuma: "Enhanced proteolytic activity is responsible for the aberrant morphogenetic development of SV40-immortalized normal human salivary gland cells grown on bascment membrane components" Lab.Invest. 70. 217-227 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 東 雅之: "SV40不死化正常ヒト唾液腺細胞における野性型p53の発現" 日本口腔科学会雑誌. 43. 580-585 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Azuma: "Proteolytic enzymes in salivary extravasation mucoceles" J.Oral Pathol.Med.24. 299-302 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Azuma: "Lack of expression of transforming growth factor-β type II receptor associated with malignant progression in human salivary gland cell clones" Int.J.Cancer. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Azuma: "Different signaling pathways involved in transforming growth factor-β1-induced morphologic change and type IV collagen synthesis in SV40 immortalized normal human salivary gland duct and myoepithelial cell clones" Arch.Oral Biol.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Azuma: "Increased matrix metalloproteinase-2 activity induced by TGF-β1 in human salivary gland duct cells is associated with the acvclopment of eyst formation in vlxo" J.Oral Pathol.Med.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masayuki Azuma: "Enhanced proteolytic activity is responsible for the aberrant morphogenetic development of SV40-immortalized normal human salivary gland cells grown on basement membrane components" Lab.Invest.70. 217-227 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 東 雅之: "SV40不死化正常ヒト唾液腺細胞における野生型P53の発現" 日本口腔科学会雑誌. 43. 580-585 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Masayuki Azuma: "Proteolytic enzymes in salivary extravasation mucoceles" J.Oral Pathol.Med.(in press).

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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