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Development of Nitroarene Analyzer

Research Project

Project/Area Number 06557121
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionKanazawa University

Principal Investigator

HAYAKAWA Kazuichi  Kanazawa University, Graduate School of Natural Science and Engineering, Associate Professor, 大学院・自然科学研究科, 助教授 (40115267)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Masayuki  Shimadzu Corporation, Analytical Applications Department, Senior researcher, 主任
MIYAZAKI Motoichi  Kanazawa University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (50009164)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsNitroarene analyzer / Chemiluminescence / HPLC / Dinitropyrene / Nitropyrene / Nitrofluoranthene / Polycyclic aromatic hydrocarbon / Airborne particulate
Research Abstract

(1) Both the nitroarene analyzer and the Ames test are necessary in the study on mutagenic nitroarenes. For the simultaneous use of these two methods, several solvent systems were tested to extract 1,3-dinitropyrene (1,3-DNP), 1,6-DNP,1,8-DNP and 1-nitropyrene (1-NP) as well as directacting mutagenic activity from airborne particulates. Benzene-ethanol, acetonitrile, ethanol and methanol gave the high efficiencies. Considering toxicities, ethanol seemed to be the best solvent.
(2) Although the nitroarene analyzer determined trace levels of 1,3-, 1,6-and 1,8-DNPs and 1-NP in extracts from a few mg of airborne particulates, the reduction of nitroarenes to corresponding aminoarenes was required before injection. To remove this tedious treatment, a reducer column packed with zinc/glass beads was on-line introduced into the system. Considering the better resolution of aminoarenes, both the reducer column and a switching valve equipped with a heart-cutting loop were introduced just before the … More ODS separation column. This system could determine the above four compounds in extracts from airborne particulatates without pre-column reduction.
(3) In the method, not only nitroarenes but also polycyclic aromatic hydrocarbons (PAHs) were quantitatively extracted from airborne partculates. To determine them simultaneously, both the nitroarene analyzer and a conventional high-performance liquid chromatograph with a fluorescence detector were combined using a switching valve. After the reduced sample solution was injected, four nitroarenes and eight PAHs were separately determined in the two different lines in 1h with high sensitivities.
(4) Several mutagenic nitroarenes such as 2-, 4-NPs, 2-nitrofluoranthene and 6-nitrochrycene were formed in air in the presence of NOx. To determine these compounds, a reducer column packed with zinc/glass beads and two ODS columns, one for the clean-up and the other for the concentration, were introduced into the nitroarene analyzer. By the system, the above four compounds were detected in air. The fact that their ratios to 1,3-, 1,6-, 1,8-DNPs and 1-NP in rural air were larger than those in urban air suggested the formation of those compounds during the transportation. However, their mutagenic contributions were not large. Less

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Kazuichi Hayakawa: "Determination of 1,3-,1,6-,1,8-Dinitropyrenes and 1-Nitropyrenes in Vehicle Exhaust Particulates" Jpn.J.Toxicol.Environ.Health. 40. 20-25 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 村橋 毅: "蛍光/化学発光検出高速液体クロマトグラフィーによる大気粉じん中の多環芳香族炭化水素及びそのニトロ誘導体の同時分析" 分析化学. 43. 1017-1019 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 早川和一: "総説:生活環境化学物資を対象とする新規光学検出液体クロマトグラフィーの開発" 薬誌. 114. 669-680 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Determination of 1,3-,1,6-,and 1,8-Dinitropyrenes and 1-Nitropyrene in Urban Air by HPLC Using Chemiluminescence Detection" Environ.Sci.Technol.29. 928-932 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Murahashi: "Diurnal Concentrations of 1,3-,1,6-,and 1,8-Dinitropyrenens,1-Nitropyren and Benzo [a] pyrene in Air in Downtown Kanazawa and the Contribution of Disel-Engined Vehicles" Jpn.J.Toxicol.Environ.Health. 41. 328-333 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Distributions of Nitropyrenes and Mutagenicity in Airborne Particulates Collected with an Andersen Sampler" Mutation Res.348. 57-61 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa, Mizuka Butoh, Yukiko Hirabayashi, Motoichi Miyazaki: "Determination of 1,3-, 1,6-, 1,8-Dinitropyrenes and 1-Nitropyrene in Vehicle Exhaust Particulates" Jpn. J.Toxicol. Environ. Health. 40 (1). 20-25 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Murahashi, Kazuichi Hayakawa, Yoko Iwamoto, Motoichi Miyazaki: "Simultaneous Determination of Polycyclic Aromatic Hydrocarbons and Their Nitrated Derivatives in Airborne Particulates by HPLC with Fluorescence/Chemiluminescence Detection" Bunseki Kagaku. 43 (11). 1017-1020 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "(Review) Development of New Photometric Detection in Liquid Chromatography for the Determination of Environmental Compounds" Yakugaku Zasshi. 114 (9). 669-680 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa, Tsuyoshi Murahashi, Mizuka Butoh, Motoichi Miyazaki: "Determination 1,3-, 1,6-, and 1,8-Dinitropyrenes and 1-Nitropyrene in Urban Air by High-Performance Liquid Chromatography Using Chemiluminescence Detection" Environ. Sci, Technol.29 (4). 928-932 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Murahashi, Motoichi Miyazaki, Ryuichi Kakizawa, Yoshihisa Yamagishi, Moritsugu Kitamura, Kazuichi Hayakawa: "Diurnal Concentrations of 1,3-, 1,6-, 1,8-Dinitropyrenes, 1-Nitropyrene and Benzo[a]-pyrene in Air in Downtown Kanazawa and the Contribution of Diesel-Engine Vehicles" Jpn. J.Toxicol. Environ. Health. 41 (5). 328-333 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa, Yoshitatsu Kawaguchi, Tsuyoshi Murahashi, Motoichi Miyazaki: "Distribution of Nitro-pyrenes and Mutagenicity in Airborne Particulates Collected with an Andersen Sampler" Mutation Res.348. 57-61 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kazuichi Hayakawa: "Determination of 1,3-,1,6-,1,8-Dinitropyrenes and 1-Nitropyrene in Vehicle Exhaust Particulates" Jpn. J. Toxicol. Environ. Health. 40. 20-25 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 村橋毅: "蛍光/化学発光検出高速液体クロマトグラフィーによる大気粉じん中の多環芳香族炭化水素及びそのニトロ誘導体の同時分析" 分析化学. 43. 1017-1019 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 早川和一: "総説:生活環境化学物質を対象とする新規光学検出液体クロマトグラフィーの開発" 薬誌. 114. 669-680 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuichi Hayakawa: "Determination of 1,3-,1,6-,and 1,8-Dinitropyrenes and 1-Nitropyrene in Urban Air by HPLC Using Chemiluminescence Detection" Environ. Sci. Technol.29. 928-932 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuyoshi Murahashi: "Diurnal Concentrations of 1,3-,1,6-,1,8-Dinitropyrenes,1-Nitropyren and Benzo[a]-pyrene in Air in Downtown Kanazawa and the Contribution of Diesel-Engined Vehicles" Jpn. J. Toxicol. Environ. Health. 41. 328-333 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuichi Hayakawa: "Distributions of Nitropyrenes and Mutagenicity in Airborne Particulates Collected with an Andersen Sampler" Mutation Res.348. 57-61 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kazuich Hayakawa: "Theoretical Consideration on Polarized Photometric Detection" Biomed Chromatogr.8. 130-133 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 早川和一: "総説:生活環境化学物質を対象とする新規光学検出液体クロマトグラフィーの開発" 薬誌. 114. 669-680 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 村橋 毅: "蛍光/化学発光検出高速液体クロマトグラフィーによる大気粉じん中の多環芳香族炭化水素及びそのニトロ誘導体の同時分析" 分析化学. 43. 1017-1019 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuich Hayakawa: "Behavior of Mutagenic Nitroaremes in Urban Air" Jpn.J.Toxicol.Environ.Health. 41. P-13 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazuich Hayakawa: "Determination of 1,3-,1,6-,and 1,8-Dinitropyrenes and 1-Nitropyrene in Urban Air by HPLC Using Chemiluminescence Detection" Environ.Sci.Technol.29(in Press). (1995)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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