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Development of in vivo ESR for detecting free radicals in human living body

Research Project

Project/Area Number 06557123
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Physical pharmacy
Research InstitutionKyushu University

Principal Investigator

UTSUMI Hideo  Faculty of Pharmaceutical Sciences, Kyusyu University, Professor, 薬学部, 教授 (20101694)

Co-Investigator(Kenkyū-buntansha) FUJII Kanae  JEOL,The chief of a section, 研究開発部, 課長
OZAWA Toshihiko  Department of Bioregulation Research National Institute of Radiological Science,, 薬理化学部, 部長 (40160858)
KIRINO Yutaka  Faculty of Pharmaceutical Sciences, The University of Tokyo, Professor, 薬学部, 教授 (10012668)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 1996: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1995: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1994: ¥8,500,000 (Direct Cost: ¥8,500,000)
KeywordsElectron Spin Resanance / Free Radical / Active Oxygen / in vivo / ESR-CT / ラット / 関節炎 / 糖尿病 / ニトロキシドラジカル / 診断機器 / SOD / 遺伝子組換えマウス / ESR / 活性酵素 / 画像解析 / イメージング / 生体レドックス / 一酸化窒素 / 電子スビン共鳴(ESR) / 一酸化窒素(NO)
Research Abstract

ESR is a unique method to detect free radical reactions. The application of ESR was limited to small animals such as mice, however. In this study, 300 MHz ESR apparatus was developed, which is designed to measure in vivo free radical reactions in arm of human. Magnet, resonator and microwave unit were redesigned to measure large animals. This mewly developed ESR apparatus was manufactured and the following research was carried out with this new ESR apparatus.
1.In rat adjuvant arthritis model, free radical reactions increased in adjuvant treated group than control group. The increment was correlated with the degree of oedema and was inhibited by treatments of Cu, Zn-SOD.Imaging of ESR-CT demonstrated that free radical reactions increased inflamed part of rat adjuvant arthritis.
2.In rat diabetes model induced by streptzotocin, free radical reactions in upper abdomen increased compared with controlgroup, and this increment was inhibited by Cu, Zn-SOD treatment.
3.This medium-sized ESR apparatus enabled to detect free radicals attached on arm of human, indicating that free radical reactions in human can be monitored with newly developed 300 MHz ESR apparatus.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Yuri Miura,et al.: "In Vivo ESR Studies of Antioxidant Activity on Free Radical reaction in Living Mice under Oxidative Stress" Free Rad.Res.22(3). 209-214 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi,et al.: "In vivo ESR measurements of free radical reactions in living mice" Toxicology Letters. 82/83. 561-565 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuo Yamaguchi,et al.: "In vivo ESR studies on pharmacokinetics and metabolism of parenteral lipid emulsion in living mice" Pharm.Res.13(5). 729-733 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomokazu Yoshida,et al.: "Free radicals from 1-palmotoyl-2-arachidonoyl phosphatidylcholine lippsomes in Fe^<2+>/ascorbic acid solution" Biol.Pharm.Bull.19(16). 779-782 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] V.Quaresima,et al.: "In vivo Detection Mouse Liver Nitric Oxide Generation by Spin Trapping Electron Paramagnetic Resonance Spectroscopy" Biochem.Biophys.Res.Commun.221. 729-734 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi,et al.: "In vivo ESR measurements of free radical reactions in living animals" Curr.Topics Biophys. 20. 41-45 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi,et al.: "Bioradcals detected by ESR spectroscopy" H.Ohya-Nishikawa & L.Packer, 14 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 竹下啓蔵,et al.: "バイオサイエンスESR" 桜井弘, 46 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yuri Miura, et al.: "In Vivo ESR Studies of Antioxidant Activity on Free Radical reaction in Living Mice under Oxidative Stress" Free Rad.Res.22(3). 209-214 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi, et al.: "In Vivo ESR measurements of free radical reactions in living mice" Toxicology Letters. 82/83. 561-565 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tetsuo Yamaguchi, et al.: "In Vivo ESR studies on pharmacokinetics and metabolism of parenteral lipid emulsion in living mice" Pharm.Res.13(5). 729-733 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomokazu Yoshida, et al.: "Free radicals from 1-palmitoyl-2-arachidonoyl phosphatidylcholine liposomes in Fe^<2+>/ascorbic acid solution" Biol.Pharm.Bull.19(16). 779-782 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] V.Quaresima, et al.: "In vivo Detection Mouse Liver Nitric Oxide Generation by Spin Trapping Electron Paramagnetic Resonance Spectroscopy" Biochem.Biophys.Res.Commun.221. 729-734 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi, et al.: "In vivo ESR measurements of free radical reactions in living animals" Curr.Topics Biophys. 20. 41-45 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi, et al.: IBioradcals detected by ESR spectroscopy. Ed.by H.Ohya-Nishikawa & L.Packer, 321-334 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Keizo Takeshita, et al.: Bioscience ESR. Ed.by Hiromu Sakurai, 89-106 175-188 495-508 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hideo Utsumi et al.: "In Vivo ESR Measurement of Free Radical Reactions in Living Animals" Current Topics in Biophysics. 20(1). 41-45 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hideo Utsumi et al.: "In Vivo ESR Measurements of Free Radical Reactions in Living Mice" J.Toxicol.Sci.21(5). 293-295 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tomokazu Yoshida et al.: "Free Radicals from I-Palmitoy1-2-arachidonoy/phosphatidy/choline Liposomes in Fe^<2+>/Ascorbic Acid Solution" Biol.Pharm.Bull.19(6). 779-782 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tetsuo Yamaguchi et al.: "In Vivo ESR Studies on Pharmacokinetics and Metabolism of Parenteral Lipid Emulsion in Living Mice" Pharmaceu.Res.13(5). 729-733 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松本謙一郎 他: "アンスラリン塗布マウス皮膚へのUVA照射時のラジカル発生部位の解析" 磁気共鳴と医学. 7. 102-105 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 竹下啓蔵 他: "マウス肺内ニトロキシルラジカル還元におけるSH化合物の役割-LバンドESRを用いたwhole body測定による検討" 磁気共鳴と医学. 7. 165-168 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 三浦ゆり 他: "バイオサイエンスESR 1 5.3スピンラベルの速度論的応用" 廣川書店, 18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松本謙一郎 他: "バイオサイエンスESR 1 5.8ESRの画像化法(A)" 廣川書店, 15 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Yoshida,H.otake,et.al and H.Utsumi: "Free radicals from 1-palmitoyl-2-arachidonoyl phosphatidylcholine liposomes in Fe^<2+>/ascorbic acid solution" Chem.Pharm.Bull.,. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Yamaguchi,S.Itai,et.al and H.Utsumi: "In vivo ESR studies on pharmacokinetics and metabolism of parenteral lipid emulsion in living mice" Pharm,Res.,. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Utsumi,K,Ichikawa,and K.Takeshita: "In vivo ESR measurements of free radical reactions in living mice" Toxicology Letters. 82/83. 561-565 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K-I.Matsumoto,A.Hamada,and H.Utsumi: "Simultaneous ESR Imaging of spin Probes Encapsulated into and Released from Liposomes" Mag.Res.Med.6. 71-73 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K,Takeshita,A.Hamada and H.Utsumi: "L-Band ESR Study on Mechanism of Radical Reduction in Mouse Lung" Mag,Res,Med.6. 255-257 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y.Miura,H.Utsumi,and A,Hamada: "Estimation of In vivo Antioxidant Activity by Using Redox Reaction of Nitroxide Radical with In vivo ESR" Mag.Res.Med.6. 252-254 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Utsumi,K.Takeshita: "Biovadicals detected by ESR spectroscopy" Birkhaeuser Verlag,Basel, 14 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hideo Utsumi,Koji Kiyoshige,et al.: "Comparative Studies on Cytotoxicity of Micropollutants in Water:Principle of Cytotoxicity Matrix" Environ,Toxicol,Water Quality. 9. 333-339

    • Related Report
      1994 Annual Research Report
  • [Publications] Masaji Inoue,Hideo Utsumi,et al.: "A Comparative ESR Study of Some Paramagnetic Materials as Probes for the Noninvasive Measurement of Dissolved Oxygen in Biological Systems" Chem.Pharm,Bull.42. 2346-2348

    • Related Report
      1994 Annual Research Report
  • [Publications] Yuri Miura,Hideo Utsumi et al.: "IN VIVO ESR Studies of Antioxidant Activity on Free Radical Reaction in Living Mice under Oxidative Stress" Free Rad.Res.Commun.in press-

    • Related Report
      1994 Annual Research Report
  • [Publications] 内海英雄: "生体計測用ESRによる生体内フリーラジカル反応の無侵襲解析" フリーラジカルの臨床. 8. 91-98

    • Related Report
      1994 Annual Research Report
  • [Publications] 内海英雄: "ESRによる生体フリーラジカルの測定" 衛生化学. 40. 213-224

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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