| Project/Area Number |
06557126
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
SAITO Kazuki Chiba University, Faculty of Pharmaceutical Sciences, Professor, 薬学部, 教授 (00146705)
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| Co-Investigator(Kenkyū-buntansha) |
MASUTA Chikara Chiba University, Faculty of Pharmaceutical Sciences, Lecturer, 農学部, 助教授
YAMAZAKI Mami Chiba University, Faculty of Pharmaceutical Sciences, Lecturer, 薬学部, 講師 (70222370)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Genetic engineering / Environment / Moleculart cloning / Sulfur resistance / Amino acids / Sulfur metabolism / システイン生合成 / 大気汚染 / 環境科学 / トランスジェニック植物 |
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| Research Abstract |
Cysteine biosynthesis is the major assimilation mechanism of sulfur in plants. Cysteine synthase (CSase) [O-acetylserine (thiol) -lyase] responsible for the terminal step of cysteine biosynthesis catalyzes the formation of L-cysteine from O-acetyl-L-serine (OAS) and hydrogen sulfide. OAS is supplied by serine acetyltransferase (SATase) from acetyl-CoA and serine. There are at least three isoforms of CSases in the plant cells, which are localized in cytoplasm, chloroplasts and mitochondria, respectively. The subcellular localization of these isoforms was confirmed by in vivo experiments using transgenic plants expressing the transit peptide-gus fusion genes. Transgenic tobacco plants, integrated with the constructs for 35S-CSaseA cDNA,35S-pearbcS transit peptide-CSaseA cDNA and 35S-antisense CSaseA cDNA,were obtained and analyzed for modulation of cysteine biosynthesis in responese to various sulfur stress. The results indicated that over-accumulated foreign cysteine synthase in chloroplasts could enhance biosynthetic flow of cysteine.
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