Project/Area Number |
06557131
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 試験 |
Research Field |
Biological pharmacy
|
Research Institution | TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE |
Principal Investigator |
SAIDO Takaomi C Tokyo Metropolitan Institute of Medical Science, Department of Molecular Biology, Research Scientist, 遺伝情報研究部門, 研究員 (80205690)
|
Co-Investigator(Kenkyū-buntansha) |
SHIBATA Masao Medical and Biological Laboratory, Inc., Department of Medicine, Chief, 医薬開発研究室, 室長
|
Project Period (FY) |
1994 – 1996
|
Project Status |
Completed (Fiscal Year 1996)
|
Budget Amount *help |
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
|
Keywords | PROTEOLYSIS / PROTEASE / CALPAIN / FODRIN / ISCHEMIA / beta-AMYLOID / AGING / ALZHEIMER'S DISEASE / プロテオリミス / フキドリン / フオドリン |
Research Abstract |
Biological science has long suffered from the technical inability to spatially resolve proteolytic phenomena ; orthodox methods require sample homogenization resulting in loss of spatial information that could reveal where in cells or tissues a proteolytic reaction of interest takes place. Efforts have thus been made to overcome this obstacle by developing antibodies that distinguish a proteolyzed form of a given protein from its intact form. The methodology for such an approach has been generalized and is used as a powerful tool to resolve proteolytic phenomena such as calpain-catalyzed proteolysis in brain ischemia and processing of beta-amyloid peptide associated with Alzheimer's disease. Newly obtained information by this method is likely to contribute to developint preventive and therapeutic measures against these neurodegenerative disorders.
|