| Project/Area Number |
06557131
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Biological pharmacy
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| Research Institution | TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE |
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Principal Investigator |
SAIDO Takaomi C Tokyo Metropolitan Institute of Medical Science, Department of Molecular Biology, Research Scientist, 遺伝情報研究部門, 研究員 (80205690)
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| Co-Investigator(Kenkyū-buntansha) |
SHIBATA Masao Medical and Biological Laboratory, Inc., Department of Medicine, Chief, 医薬開発研究室, 室長
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥9,400,000 (Direct Cost: ¥9,400,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
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| Keywords | PROTEOLYSIS / PROTEASE / CALPAIN / FODRIN / ISCHEMIA / beta-AMYLOID / AGING / ALZHEIMER'S DISEASE / プロテオリミス / フキドリン / フオドリン |
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| Research Abstract |
Biological science has long suffered from the technical inability to spatially resolve proteolytic phenomena ; orthodox methods require sample homogenization resulting in loss of spatial information that could reveal where in cells or tissues a proteolytic reaction of interest takes place. Efforts have thus been made to overcome this obstacle by developing antibodies that distinguish a proteolyzed form of a given protein from its intact form. The methodology for such an approach has been generalized and is used as a powerful tool to resolve proteolytic phenomena such as calpain-catalyzed proteolysis in brain ischemia and processing of beta-amyloid peptide associated with Alzheimer's disease. Newly obtained information by this method is likely to contribute to developint preventive and therapeutic measures against these neurodegenerative disorders.
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