| Project/Area Number |
06557146
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory medicine
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| Research Institution | Osaka University |
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Principal Investigator |
TANIGUCHI Naoyuki Osaka Univ.Med.Sch.Professor, 医学部, 教授 (90002188)
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| Co-Investigator(Kenkyū-buntansha) |
TSUKADA Yutaka Hachioji Res.Lab., S.R.L.Ltd.Researcher, 八王子ラボラトリー, 副所長
KONDO Takahito Nagasaki Uni.Med.Sch.Professor, 医学部, 教授 (00158908)
SUZUKI Keiichiro Osaka Univ.Med.Sch.Res.Associate, 医学部, 助手 (70221322)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥8,300,000 (Direct Cost: ¥8,300,000)
Fiscal Year 1995: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | Adult respiratory distress syndrome (ARDS) / Manganese superoxide dismutase / Sepsis / 酵素免疫測定法(ELISA) / Mn-SOD / ARDS / モノクローナル抗体 |
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| Research Abstract |
Adult respiratory distress syndrome (ARDS) can develop as a complication of various disorders, including sepsis, but it has not been possible to identify which of the patients at risk will develop serious disorfer. We have established a more rapid and easy method to measure serum levels of manganese superoxide dismutase than before and investigated the ability of six markers, measured sequentially in blood, to predict development of ARDS in patients with sepsis.
At the initial diagnosis of sepsis (6-24h before the development of ARDS), serum manganese superoxide dismutase concentration and catalase activity were higher in the 6 patients who subsequently developed ARDS than in patients who did not develop ARDS.These changes in antioxidant enzymes predicted the development of ARDS in septic patients with the same sensitivity, specificity, and efficiency as simultaneous assessments of serum lactate dehydrogenase activity and factor VIII concentration. By contrast, serum glutathione peroxidase activity and alpha Pi-elastase complex concentration did not differ at the initial diagnosis of sepsis between patients who did and did not subsequently develop ARDS,and were not as effective in predicting the development of ARDS.
Measurement of manganese superoxide dismutase, in addition to the other markers, should facilitate identification of patients at highest risk of ARDS and allow prospective treatment.
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