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Development of Methodology for Evaluating Toxicity of Pesticides by Responses of Animal Cells

Research Project

Project/Area Number 06558080
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 環境影響評価(含放射線生物学)
Research InstitutionUniversity of Tokyo

Principal Investigator

SAKODA Akiyoshi  Univ. of Tokyo, Inst. of Ind. Sci. Associate Professor, 生産技術研究所, 助教授 (30170658)

Co-Investigator(Kenkyū-buntansha) MIYABE Kanji  Toyama Univ. Associate Professor, 教養学部, 助教授
SAKAI Yasuyuki  Univ. of Tokyo, Inst. of Ind. Sci. Research Associate, 生産技術研究所, 助手 (00235128)
SUZUKI Motoyuki  Univ. of Tokyo, Inst. of Ind. Sci. Professor, 生産技術研究所, 教授 (10011040)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 1996: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1994: ¥4,500,000 (Direct Cost: ¥4,500,000)
KeywordsAnimal Cell / Pesticide / Toxicity / Bioassay / 神経細胞 / 形態変化 / 水環境 / 毒性評価 / 肝細胞 / 機能変化
Research Abstract

The environmental waters which serve as also the reservoirs of our drinking water are contaminated by a wide variety of chemicals emitted from human activities. This fact means that the quick development of the methodology for evaluating the toxicity of water is needed. The toxicity evaluation method using living animal cells is considered to be most reliable in the practical sense. In this work, the toxicity evaluation methods on the basis of the responses of animal cells were developed by employing the pesticides as model pollutants.
1. Toxicity evaluation by functional changes of animal cells
The toxicity evaluation on the basis of functional changes such as albumin secretion of primary-cultured ret hepatocytes was developed. Also, the simplified mathematical model was proposed for describing the cellular responses to the pesticide loading. The proposed methodology was applied to toxicity evaluation of the practical river water and its rationality was validated in part.
2. Toxicity evaluation by morphological changes of animal cells
The toxicity evaluation was tried by quantitatively measuring the morphological changes of PC-12 cells when exposed to the pesticides having neurotoxicity, such as DDVP.It was found that the shrinkage of PC-12 is sensitive enough to detect and evaluate the neuroxicity of the pesticides.
3. Simple and rapid toxicity evaluation
The basic cellular function for taking LDL was focussed as a very quick indicator of the toxicity. This idea made it possible to shorten the toxicity evaluation time from the order of magnitude of day to hour.
4. Water quality evaluation in drinking water treatments by cytotoxicity
The water quality in drinking water treatment experimental set-up consisting of chlorination, ozonation and activated carbon adsorption was assayd by thus proposed bioassay. It can be concluded that bioassay should be included in routine works for water quality control at drinking water treatments.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Kazuhiro Ichikawa: "Primary Culture of Rat Hepatocytes Using Membrane-surported Collagen Sandwich with Hormon-free Medium" Biotech.Tech.8(6). 385-388 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuhiro Ichikawa: "Long-term Cytotoxicities of Various Pesticides Evaluated by Albumin -Secretion of Primary Cattured Rat Hepato cytes" Biotech.Tech.10(2). 99-102 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yasuyuki Sakai: "Quantitative Comparison of Rat Hepatocyte Functions in Two Improved Culture System with or without Rat Liver Epithelial Cell Line" Cytotechnology. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 酒井 康之: "動物細胞を用いた環境水の生体影響評価の試み" 生産研究. 47(4). 216-219 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鈴木基之: "培養神経細胞の形態変化を指標とした農薬類の毒性評価" 生産研究. 48(3). 147-150 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 鈴木基之: "ヒト細胞の代謝異常に着目した環境汚染物質の迅速毒性評価" 生産研究. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ichikawa et al.: "Primary Culture of Rat Hepatocytes Using Membrane-supported Collagen Sandwich with Hormone-free Medium" Biotech., Tech.8 (6). 385-388 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Ichikawa et al.: "Loug-term Cytotoxicities of Various Pesticides Evaluated by Albumin Secretion of Primary Cultured Rat Hepatocytes" Biotech., Tech.10 (2). 99-102 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Sakai et al.: "Quantitative Comparison of Rat Hepatocyte Functions in Two Improved Culture System with or without Rat Liver Epithelial Cell Line" Cytotechnology. 21. 243-252 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Sakai et al.: "Toxicity Evaluation of Environmental Waters by Using Animal Cells (in Japanese)" Seisan Kenkyu. 47 (4). 216-219 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Suzuki et al.: "Neurotoxicity Evaluation by Morphological Changes of PC-12 Cells (in Japanese)" Seisan Kenkyu. 48 (3). 147-150 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Suzuki et al.: "Rapid Toxicity Evaluation for Environmental Pollutants by Using Human Cells (in Japanese)" Seisan Kenkyu. in press. (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kazuhiro Ichikawa: "Primary Cutture of Rat Hepatocytes Using Membrane-suported Collagen Sardwich with Hormone-free Medium" Biotech.Tech.8(6). 385-388 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuhiro Ichikawa: "Long-term Cytotoxicities of Various Pesticides Evaluated by Albumin Secretion of Primary Cultured Rat Hepatocytes" Biotech.Tech.10(2). 99-102 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yasuyuki Sakai: "Quantitative Comparison of Rat Hepatocyte Functions in Two Improved Culture System with or without Rat Liver Epithelial Cell Line" Cytotechnology. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 酒井 康行: "動物細胞を用いた環境水の生体影響評価の試み" 生産研究. 47(4). 216-219 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 基之: "培養神経細胞の形態変化を指標とした農薬類の毒性評価" 生産研究. 48(3). 147-150 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 鈴木 基之: "ヒト細胞の代謝異常に着目した環境汚染物質の迅速毒性評価" 生産研究. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ichikawa, K, Y. Sakai, A. Sokoda, M. Suzuki: "Lonrg-term Cytotoxicities of Various Pesticides Evaluated by Albumin Secretion of Primary Cultured Rat Hepatocytes" Biotech. Tech.10. 99-102 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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