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Development of novel X-ray diffraction measurement for biological macromolecular assemblies

Research Project

Project/Area Number 06558102
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field Biophysics
Research InstitutionOsaka University (1995-1996)
The University of Tokushima (1994)

Principal Investigator

TSUKIHARA Tomitake  Osaka University, Institute for Protein Research, Professor, 蛋白質研究所, 教授 (00032277)

Co-Investigator(Kenkyū-buntansha) 佐藤 孝雄  徳島大学, 工学部, 助手 (80243731)
森本 幸生  徳島大学, 工学部, 助教授 (80200450)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥18,900,000 (Direct Cost: ¥18,900,000)
Fiscal Year 1996: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1995: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1994: ¥11,000,000 (Direct Cost: ¥11,000,000)
KeywordsBiological macromolecular assemblies / Crystal structure analysis / X-ray diffraction / Proteins / X線回折 / 回折強度測定法 / チトクロム酸化酵素 / 立体構造 / 超分子複合体
Research Abstract

Unit cell volumes of biological macromolecular assemblies are as large as more than ten times of those of protein crystals. There remain several problems to be solved in x-ray diffraction experiments for the biological macromolecular assemblies. In the case of crystals with large unit cell, distances between diffraction spots so short that it is difficult to measure intensity of each diffraction separately. Since intensity of each diffraction spot is proportional to reciprocal of unit cell volume of crystal, diffraction from crystals with large unit cells is too weak to measure their intensity accurately. Thus we have initiated this research to develop new methods to collect-x-ray diffraction data of the biological macromolecular assemblies.
Results obtained in the present research are summarized as follows : Small angle oscillation method about any direction splits each diffraction spot from the others and increases S/N of intensity data. Consequently, intensity data of crystals with unit cell dimensions of 500 is able to carried out by the method with 0.5 degree oscillation. The small angle oscillation method does not enable to decrease overlapping between diffraction spots, but also increase accuracy of intensity of each diffraction. The oscillation method about any direction reduces systematic error in observed intensity data.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] T. Tsukihara et al.: "The Whole Structure of the 13-Subinit Oxidized Cytochrome c Oxidase" Science. 272. 1136-1144 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Tsukihara et al.: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase" Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 月原冨武: "呼吸酵素チトクロム酸化酵素のX線結晶構造解析" 遺伝. 50巻. 11-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 月原冨武: "巨大な膜タンパク質複合体の立体構造の解明" 日本物理学会誌. 51巻. 560-509 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 青山浩,月原冨武: "ウシ心筋のチトクロム酸化酵素の金属中心の構造" 日本結晶学会誌. 38巻. 48-53 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Morimoto: "Ordered Structure of the Crystallized BOvine 20S Proteasome." J. Biochem.177. 471-474 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tsukihara, H.Aoyama, E.Yamashita, T.Tomizaki, H.Yamaguchi, K.Shinzawa-Itoh, R.Nakashima, R.Yaono & S.Yoshikawa: "The Whole Structure of the 13-subunits Oxidized Cytochrome c Oxidase." Science. 272. 1136-1144 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tsukihara, H.Aoyama, E.Yamashita, T.Tomizaki, H.Yamaguchi, K.Shinzawa-Itoh, R.Nakashima, R.Yaono & S.Yoshikawa: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase." Science. 269. 1069-1074 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tsukihara: "X-ray Crystal Structure Analysis of a Respiratory Enzyme, Cytochrome c Oxidase." Iden. 50. 11-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tsukihara: "Structure Analysis of a Large Membrane Protein Complex." Nihon Butsuri Gakkai Shi. 51. 506-509 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Aoyama & T.Tsukihara: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase." Nihon Kessho Gakkai Shi.38. 48-53 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Morimoto, M.Mizushima, A.Yagi, N.Tanahashi, K.Tanaka, A.Ichihara & T.Tsukihara: "Ordered Structure of the Crystallized Bovine 20S Proteasome." J.Biochem.117. 471-474 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T.Tsukihara et al.: "The Whole Structure of the 13-Subinit Oxidized Cytochrome c Oxidase" Scince. 272. 1136-1144 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Tsukihara et al.: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase" Scince. 269. 1069-1074 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 月原冨武: "呼吸酵素チトクロム酸化酵素のX線結晶構造解析" 遺伝. 50巻. 11-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 月原冨武: "巨大な膜タンパク質複合体の立体構造の解明" 日本物理学会誌. 51巻. 560-509 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 青山浩,月原冨武: "ウシ心筋のチトクロム酸化酵素の金属中心の構造" 日本結晶学会誌. 38巻. 48-53 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Morimoto: "Ordered Structure of the Crystallize6 Bovine 20S Proteasome." J.Biochem.177. 471-474 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsukihara,T.et al.: "Structure of Metal Sites of Oxidized Bovine Heart Cytochrome c Oxidase at 2.8A" Science. 269. 1069-1074 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsukihara,T.et al.: "The Whole Structure of the 13 Subunit Oxidized Cytochrome c Oxidase at 2.8A" Science. (in press). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Bando et al: "Crystal structural analysis of Tobacco Necrosis Virus at 5Å resolution" Acta.Cryst.D50. 878-883 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 山下栄樹,月原富武: "巨大分子結晶学-超分子複合体への挑戦-" 日本結晶学会誌. 36. 294-296 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 月原富武: "X線結晶構造解析" 蛋白質・核酸・酵素. 39. 1263-1273 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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