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Development of a method for making a gene expression map of animal embryos

Research Project

Project/Area Number 06558105
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Developmental biology
Research InstitutionThe University of Tokushima

Principal Investigator

NOJI Sumihare  Faculty of Engineering, University of Tokushima, Professor Assistant Professor Assistant, 工学部, 教授 (40156211)

Co-Investigator(Kenkyū-buntansha) OHUCHI Hideyo  Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助手 (00253229)
YOSHIOKA Hidefumi  Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助教授 (40191548)
OYAMA Akihiro  ALOKA
大山 晃弘  アロカ株式会社, 研究所, 研究員
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1995: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1994: ¥7,500,000 (Direct Cost: ¥7,500,000)
Keywordsin situ hybridization / cell growth factor / animal embryos / automation / gene-screening
Research Abstract

In this research project, we developed a method which can make a map for gene expression in a embryo. We first automated all processes of in situ hybridization. In the first year, we made a instrument for auto-processor of in situ hybridization with glass slides. In the second year, we made a instrument for auto-processor of whole-mount in situ hybridization. Now, we are testing the instrument. In parallel, we cloned various genes working during development. We focused on chick embryo, Drosophila embryo, cricket embryo for making a map for gene expression. We established a method for whole-mount in situ hybridization with chick and Drosophila embryos, while we have not yet succeed clear in situ hybridization results for cricket embryos. For example, we cloned especially vertebrate and cricket genes homologous to the Drosophila segment polarity gene hedgehog (hh). Shh has been reported to be involved in induction of the floor plate and motor neurons in the spinal cord, and in anteroposterior pattern formation of limbs probably in all vertebrate. Shh is supposed to be a crucial factor for intercellular Interaction such as an epithelium-mesenchymal interaction. In the present project, from maps for gene expression, we found that the Shh, BMP2, Wnt, FGF genes are expressed during various developing tissues.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] H.Ohuchi et al: "Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis." Biochem.Biophys.res.Commun.204. 882-888 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Takebayashi et al: "Hepatocyte growth factor/scatter factor modulates cell motility proliferation and proteoglycan sysntyesis of chondrocytes." J.Cell Biol.129. 1141-1419 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Ohuchi et al: "An additional limb can be induced from the flank of the chick embryoly by FGF4." Biochem.Biophys.res.Commun.209. 809-816 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Mima et al: "FGF can induce outgrowth of somatic mesoderm both inside and outside of limb-forming regions." Dev.Biol.167. 617-620 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] A.Orimo et al: "Molecular cloning,structure and expression of mouse estrogen-sponsive finger protein (efp) ; co-locatization with estrogen receptor mRNA in target organs." J.Biol.Chem.270. 24406-24413 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ishikawa et al: "Truncated type II receptor for BMP-4 induces secondary axial structures in Xenopus embryos." Biochem.Biophys.Res.Commun.216. 26-33 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野地澄晴: "生物薬科学実験講座V 遺伝子-In situ hybridization" 広川書店, 125-130 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 野地澄晴: "分子生物学プロトコール-組織切片へのIn situハイブリダイゼーション" 南江堂, 213-223 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Ohuchi et al.: "Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis." Biochem.Biophys.Res.Commun.Vol.204. 882-888 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Takebayashi et al.: "Hepatocyte growth factor/scatter factor has mitogenic, moto-genic and morphogenetic activities on chondrocytes." J.Cell Biol.Vol.129. 1411-1419 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Ohuchi et al.: "An additional limb can be induced from the flank of the chick enbryoly by FGF4." Biochem.Biophys.Res.Commun.Vol.209. 809-816 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Mima et al.: "FGF can induce outgrowth of somatic mesoderm both inside and outside of limb-forming regions." Dev.Biol.Vol.167. 617-620 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Ishikawa et al.: "Truncated type II receptor for BMP-4 induces secondary axial structures in Xenopus embryos." Biochem.Biophys.Res.Commun.Vol.216. 26-33 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] H.Ohuchi et al: "“Involvement of androgen-induced growth factor (FGF-8) gene in mouse embryogenesis and morphogenesis"." Biochem.Biophys.Res.Commun.204. 882-888 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Takebayashi et al: "Hepatocyte growth factor/scatter factor has mitogenic,motogenic and morphogenetic activities on chondrocytes." J.Cell Biol.129. 1141-1419 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Ohuchi et al: "An additional limb can be induced from the flank of chick embryoly by FGF4." Biochem.Biophys.Res.Commun.209. 809-816 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Mima et al: "FGF can induce outgrowth of somatic mesoderm both inside and outside of limb-forming regions." Dev.Biol.167. 617-620 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] A.Orimo et al: "Molecular cloning,structure and expressiun of mouse estrogen-responsive finger protein (efp) ; co-locatization with estrogen receptor mRNA in target organs." J.Biol.Chem.270. 24406-24413 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Ishikawa et al: "Truncated type II receptor for BMP-4 induces secondary axial structures in Xenopus embryos." Biochem.Biophys.Res.Commun.216. 26-33 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野地澄晴: "生物薬科学実験講座V 遺伝子-In situ hybridization" 広川書店, 6 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 野地澄晴: "分子生物学プロトコール-組織切片へのIn-situ ハイブリダイゼーション" 南江堂, 11 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] H.Ohuchi et al: "An additional limb can be induce from the flank of the chick embryo by FGF-4." Biochem.Biophys.Res.Commun.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Nohno et al: "Involvement of the Sonic hedgehog gene in chick feather formation." Biochem.Biophys.Res.Commun.206. 33-39 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Takebayashi et al: "Hepatocyte growth factor/scatter factor has mitogenic,motogenic and morphogenetic activities on chondrocytes." J.Cell Biol.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Matsuhashi et al: "A new gene,nel,encoding a Mr.93K protein with EGF-like repeats is strongly expressed in neural tissues of early stage chick embryos." Dev.Dyn.(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Ohuchi et al: "Expression patterns of turo fibroblast factor receptor genes during chick eye development." Exp.Eye.Res.58. 649-658 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Ohuchi et al: "Involvement of androgen-induced growth factor (FGF-8)gene in mouse embryogenesis and morphogenesis." Biochem.Biophys.Res.Commun.204. 882-888 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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