Development of a method for making a gene expression map of animal embryos
| Project/Area Number |
06558105
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
NOJI Sumihare Faculty of Engineering, University of Tokushima, Professor Assistant Professor Assistant, 工学部, 教授 (40156211)
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| Co-Investigator(Kenkyū-buntansha) |
OHUCHI Hideyo Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助手 (00253229)
YOSHIOKA Hidefumi Faculty of Engineering, University of Tokushima, Professor Assistant Professor A, 工学部, 助教授 (40191548)
OYAMA Akihiro ALOKA
大山 晃弘 アロカ株式会社, 研究所, 研究員
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥12,000,000 (Direct Cost: ¥12,000,000)
Fiscal Year 1995: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1994: ¥7,500,000 (Direct Cost: ¥7,500,000)
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| Keywords | in situ hybridization / cell growth factor / animal embryos / automation / gene-screening |
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| Research Abstract |
In this research project, we developed a method which can make a map for gene expression in a embryo. We first automated all processes of in situ hybridization. In the first year, we made a instrument for auto-processor of in situ hybridization with glass slides. In the second year, we made a instrument for auto-processor of whole-mount in situ hybridization. Now, we are testing the instrument. In parallel, we cloned various genes working during development. We focused on chick embryo, Drosophila embryo, cricket embryo for making a map for gene expression. We established a method for whole-mount in situ hybridization with chick and Drosophila embryos, while we have not yet succeed clear in situ hybridization results for cricket embryos. For example, we cloned especially vertebrate and cricket genes homologous to the Drosophila segment polarity gene hedgehog (hh). Shh has been reported to be involved in induction of the floor plate and motor neurons in the spinal cord, and in anteroposterior pattern formation of limbs probably in all vertebrate. Shh is supposed to be a crucial factor for intercellular Interaction such as an epithelium-mesenchymal interaction. In the present project, from maps for gene expression, we found that the Shh, BMP2, Wnt, FGF genes are expressed during various developing tissues.
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Report
(3 results)
Research Products
(27 results)
