| Project/Area Number |
06558112
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 試験 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hokkaido University |
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Principal Investigator |
ARIKAWA Jiro Hokkaido University School of Medicine, Institute for Animal Experimentation, Professor, 医学部, 教授 (10142704)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAKURA Akira Division of Microbiology ICLAS Monitoring Center, Central Laboratory of Experime, 微生物モニタリングセンター, 室長代理 (60167484)
SUGIYAMA Kazuyoshi Section of Biosafety, National Institute of Health, Japan, Chief, ウイルスー部, 室長 (90150185)
YOSHIMATSU Kumiko Hokkaido University School of Medicine, Institute for Animal Experimentation, As, 医学部, 助手 (90220722)
KARIWA Hiroaki Graduate School of Veterinary Medicine, Hokkaido University, Lecturer, 大学院・獣医学研究科, 講師 (70224714)
橋本 信夫 北海道大学, 獣医学部, 教授 (60082103)
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| Project Period (FY) |
1994 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥18,500,000 (Direct Cost: ¥18,500,000)
Fiscal Year 1996: ¥5,900,000 (Direct Cost: ¥5,900,000)
Fiscal Year 1995: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1994: ¥7,000,000 (Direct Cost: ¥7,000,000)
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| Keywords | HFRS / Hantavirus / Baculovirus / Serological diagnosis / ELISA / Westem blotting / Experimental animals / Zoonosis |
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| Research Abstract |
1. Recombinant baculovirus expressing hantavirus nucleocapsid protein (Bac.HTN-NP) was applied for the antigen of ELISA.Specificity of the ELISA was the same to that of the IFA which uses infected Vero E6 cells as antigen. However, the ELISA had a sensitivity problem because of the relatively high background coloring.
2. cDNA which encoded hantavirus nucleocapsid protein was expressed by using E.coli system and established capture ELISA system. This ELISA was shown to have same specificity and sensitivity to that of the ordinally IFA,and therefore, considered as a useful screening method.
3. Bac.HTN-NP was successfully applied for the antigen for Western blotting (WB). The WB method readily distinguished specific reaction from nonspecific reaction from the WB pattern. Therefore, the WB was considered as an effective method for the serologic confirmation of hantavirus infection.
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