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Development of Natural Type Hydroxyapatite-Collagen Composite

Research Project

Project/Area Number 06558119
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section試験
Research Field Biomedical engineering/Biological material science
Research InstitutionUNIVERSITY of TOKYO

Principal Investigator

NISHIHARA Katsunari  Department of Oral Surgery, Faculty of Medicine, University of Tokyo, 医学部・附属病院, 講師 (10010323)

Co-Investigator(Kenkyū-buntansha) HIROTA Kazushi  National Institute for Research in Inorganic Materials, 研究員
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsHydroxyapatite / Collagen / High Pressure Low Temperature Sintering / Biomechanics / Hyaluronic Acid / Major Histocompatibility Antigen / Experimental Evolutionary Study / Xenotransplantation / 骨髄造血巣 / 系統発生学 / 分子生物学 / アパタイト / 骨髄造血 / 軟骨
Research Abstract

Conventionally, hydroxyapatite sintering has been aimed at stiff, stable apatite due to chemical bonding with osseous tissue. However, direct bonding of sintered hydroxyapatite with bone may easily be destroyed under severe repeated loading just like osseointegration of metal because of differences in elastic moduli from each other. Therefore, development of stiff, stable hydroxyapatite makes no sense just as in the case of bioinert metal for biomechanical substitution.
Sintering of calcium-deficient hydroxyapatite has already been reported, which bonded water at the calcium-deficient site, and was sintered up to fully dense bodies at 300゚C under a pressure of 600MPa. In the presence of collagen in an aqueous phase, we tried tosynthesize the hydroxyapatite by means of reaction between an aqueous solution of phosphoric acid and a calcium-hydroxide suspension. A diluted collagen solution was mixed with an aqueous solution of phosphoric acid and was poured slowly into a calcium-hydroxide s … More uspended aqueous phase. No collagen was found in the mother liquid thus formed. All collagen in the solution was found to be collecting in the precipitate. Five hundred grams of commercially available collagen solution was diluted up to 8 liters and mixed with 0.6mole of phosphoric acid. The CaO was crushed into fine powder and mixed with water. The Ca (OH) aqueous suspension thus formed was mixed, and the collagen-phosphoric acid mixed solution was added slowly. The precipitate was filtered and partly dried until suitable water content formed. Then, it was mounted in a metal capsule. The capsule was evacuated and sealed by welding. It was thin kept for 8 hrs at 200 MPa at 40゚C.The resulting apatite-collagen composite was 1.75g/ml in density, 2GPa in Young's modulus, and 6.5MPa in compression strength. The specimen could be cut by a knife, and was stable against immersion in water. The physical property of this type of complex may change according to composition and treating conditions. The sample was implanted in dogs chandrichthies (shark) and histologically evalutated. Using newly development biomaterials, experimental evolutionary study is carried out. Xenotransplantations between shark and amphibian are successfully carried out. From this research implant fact is discovered that in archetype vertebrates immune system is in tolerance just like embryo of higher vertebrates. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] 西原克成、丹下 剛, 松田良一, 田中順三, 広田和士, 樺沢 洋: "人工骨髄造血巣の誘導実験と新しい免疫系の概念 -原索類・円口類・軟骨魚と哺乳類の消化系・造血系の研究比較-." 日口診誌. 9(2). 217-231 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成: "人工材料(無機)-この1年の進歩." 人工臓器. 25(649). 940-942 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成, 田中順三, 広田和士: "実験進化学手法による力学対応進化学の検証." 日口診誌. 9(2). 232-249 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成, 田中順三: "骨格系組織の力学対応システムとリモデリングの遺伝子工学" 日本機械学会第74期全国大会、平成8年9月21-24日、京都市同志社大学、日本機械学会第74期全国大会講演論文集. 155-156 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成, 田中順三: "骨性癒着と線維結合状態の天然型人工歯根の生体力学的比較研究." 第26回日本口腔インプラント学会総会、平成8年9月12-14日、岡山、第26回日本口腔インプラント学会総会抄録集. 47 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成, 藤井和子, 田中順三, 広田和士: "脊椎動物の特徴的物質と生体材料学 -Trilateral Researchと実験進化学-" 日本機械学会(No.95-26)、第4回バイオエンジニアリングシンポジウム、1995年7月25-26日、新潟、講演論文集. 115-116 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Nishihara, S.Nakagiri , ed.by P.Vincenzini: "Biomechanical Investigation of Implant Failure in Bone-Bioceramics Juncture System" Materials in Clinical Applications. 491-502 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Nishihara, K.Hirota ed.by P.Vincenzini: "Succesful Pressure Sintered of Hidroxyapatite-Collagen Composite" Materials in Clinical Applications. 297-304 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishihara, K: "Application of Bioactive Ceramics for Functional Surgery in Masticatory Organs" Bio-Medical Materials and Engineering. 4(3). 161-170 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishihara, K.and Nakagiri, S.: "Biomechanical Research on Junction System of Bone with Biomaterials." Bio-Medical Materials and Engineering. 4(3). 151-159 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nishihara, K.and Nakagiri, S.: "Biomechanical Studies on Newly Tailored Artificial Dental Root." Bio-Medical Materials and Engineering. 4(3). 141-149 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Nishihara, T.Tange, K.Hirota and K.Kawase: "Development of Hybrid Type Artificial Bone Marrow Using Sintered Hydroxyapatite" Bio-Medical Materials and Engineering. 4(1). 61-65 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 西原克成: "実験進化学と新しい免疫学の概念" 口科誌. 45(5). 617-618 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成: "骨の形態的機能適応現象のメカニズムの解明" 平成4-6年度科研費(重点領域研究)研究成果報告書. 204-205 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、丹下 剛、松田良一、瀬野久和、梁井 皎、藤井和子、田中順三、広田和士: "実験進化学手法によるハイブリッド型人工器官の開発と新しい免疫学の概念" 人工臓器. 25(3). 753-758 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、田中順三: "実験進化学手法による天然型人工歯根の開発" 第26回日本口腔インプラント学会総会抄録集. 47. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、田中順三: "骨性癒着と線維結合状態の天然型人工歯根の生体力学的比較研究" 第26回日本口腔インプラント学会総会抄録集. 47. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成: "顔面頭蓋の器官特性について" フォーラム顔学 第1回日本顔学会大会論文集 39,パネル討論:顔学への招待. (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、佐藤陽子、森沢正明: "生物の骨格系物質とW.Rouxのバイオメカニクス-マボヤによる力学対応進化学の検証" 材料力学部門講演会講演論文集. Vol.A. 133-134 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、田中順三、広田和士: "実験進化学手法による力学対応進化学の検証" 日口診誌. 9(2). 232-249 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成、丹下 剛、松田良一、田中順三、広田和士、樺沢 洋: "人工骨髄造血巣の誘導実験と新しい免疫系の概念" 日口診誌. 9(2). 217-231 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成: "人工材料(無機)-この1年の進歩" 人工臓器. 25(649). 940-942 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Nishihara,Y.Sato,M.Morisawa: "Morphology of the Viscerocranium and Evolution of Vertebrates" J Oromax Biomech. 2(1). 16-18 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Nishihara,H.Kabasawa: "Relations between Gravity and Cell Differentiation in Vertebrates -A New Concept of Immunology-" J Oromax Biomech. 2(1). 19-22 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Nishihara,J.Tanaka: "Successful inducement of hybrid type artificial bome marrow using boiceramics in various vertebrates" Bioceramics. 9. 69-72 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Nishihara: "Development of Hybrid-type artificial immune organ by means of experimental evolutionary research method using bioceramics." The 1st International Symposium of Tissue Engineering for Therapeutics Use,March 27-28,1997,Kyoto Japan,1997.招待講演.

    • Related Report
      1996 Annual Research Report
  • [Publications] 西原克成: "ハイブリッドタイプの免疫器官 -人工骨髄造血巣の誘導-" 人工臓器. 24(1). 6 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西原克成: "生体材料を用いた実験進化学手法の試み" 第17回日本バイオマテリアル学会大会予行集. 150 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西原克成: "骨の生体力学特性の解明と人工歯根周囲骨の改造" 第40回日本口腔外科学会総会抄録集. 144 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Nishihara S. Nakagiri: "Biomechanical Investigation of Implant Failure in Bone-Bioceramics Juncture System" Materials in Clinical Applications. 491-502 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Nishihara K. Hirota: "Succesful Pressure Sintering of Hidroxyapatite-Collagen Composite" Materials in Clinicla Applications. 297-304 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Fujii K. Hirota Y. Suetsugu J. Tanaka S. R. Kim K. Nishihara: "Low-temperature Sintering of Apatite and Apatite-collagen Complex" Abstracts of Second International Syposium on Apatite. 41 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Nishihara: "What is the Viscerocranium from the Standopoint of Vertebrate Evolution?" J Oromax Biomech. 1(1). 73-78 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西原克成: "顔の科学" 日本教文社, 185 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 西原克成、広田和士: "常温加圧法によるcollagen-apatite天然型人工骨の開発" 第48回日本口腔科学会総会、別府、4月21、22日、口科誌. 43(5). 819-820 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西原克成: "生体材料研究の必要充分条件" 第16回バイオマテリアル学会、山梨、1994年11月4,5日、予稿集. 83. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 瀬野久和、梁井皎、西原克成: "肋骨内,大腿骨内、筋肉内におけるアパタイト周囲の組織反応の研究" 第2回顎顔面バイオメカニクス学会、東京、1994年11月18,19日、 講演論文集. 65-66 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西原克成: "ハイブリッドタイプの免疫器官-人工骨髄造血巣の誘導-" 第32回人工臓器学会、東京、1994年10月12,13日、大会予稿集. S-21. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西原克成: "ハイブリッドタイプの人工関節を持つ人工歯根の開発" 第32回人工臓器学会、東京、1994年10月12,13日、大会予稿集. S-173. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 西原克成: "関節の種類と機能および生体力学的意義" 第21回日本臨床バイオメカニクス学会、新潟、1994年10月21,22日、抄録集. 98. (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Nishihara,K.Hirota: "Succesful Pressure Sintered of Hidroxyapatite-Collagen Complex." Proceedings of the 8th CIMTEC-World Ceramics Congress and Forum on New Materials. (in print). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kazushi Hirota,Katsunari Nishihara and Hidehiko Tanaka: "Pressure Sintering Apatite-collagen." Bio-Medical Materials and Engineering. 3(3). 147-151 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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