• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Establishment of the strain of the invertebrate animals and the analysis and conservation of its gene

Research Project

Project/Area Number 06559005
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section試験
Research Field 広領域
Research InstitutionThe University of Tokyo

Principal Investigator

MORISAWA Masaaki  Univ Tokyo.Grad.Sch.Sci.Prof, 理学部, 教授 (40013594)

Co-Investigator(Kenkyū-buntansha) GOTO Taichiro  Mie Univ.Fac.Educ.Associate Prof, 教育学部, 助教授 (90183813)
AMEMIYA Shonan  Univ.of Tokyo.Grad.Sch.Sci.Prof., 大学院・理学系研究科, 教授 (30011670)
MICHIBATA Hitoshi  Hiroshima Univ.Fac.Sci., 理学部, 教授 (00111740)
YAMAMOTO Masamichi  Okayama Univ.Fac.Sci.Prof., 理学部, 教授 (50011565)
HOSHI Motonori  Tokyo Inst.Tech.Dept.of life Sci.Prof., 生命理工学部, 教授 (20012411)
佐藤 矩行  京都大学, 理学部, 教授 (30025481)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥16,700,000 (Direct Cost: ¥16,700,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1995: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1994: ¥9,400,000 (Direct Cost: ¥9,400,000)
KeywordsAscidians / Amphioxus / Sea-lilies / Arrow worms / Actin gene / HOX gene / Gene library / Genetic polymorphism / Hox遺伝子 / ユウレイボヤ / 自家交配系 / 染色体解析 / マナマコ / 白色マボヤ / cDNAライブラリー / 海産動物飼育培養装置 / カタユウレイボヤ / ヒトデ純系 / アスキジア科ホヤ / 継代飼育 / ゲノムライブラリー
Research Abstract

Establishment of the invertebrate strain : Amphioxus which is becomming extinct were tried to collect at the various area in Japan and China and the obtained animals were reared and bred using tartificial insemination at the Misaki Marine Biological Station, the Univ.of Tokyo. Furthermore, the investigators of MMBS have investigated the sea shore with research vessels and found and caught 3 individuals suggesting that a few number of the animals still inhabit in the area of Japan and possibility to preserve the species is still remains, Oikopleura of which the developmental process has been obscure was collected and reared in the aquarium for a while. Artificial insemination was conducted and the embryo could keep for a long peiods. Platyhelminthes Convoluta naikaiensis which is the primitive triplobrastica is the phylogenicallyinteresting animai species. Establishment of the strain and the method for collection, rearing and artificial fertilization is focused in the Ushimadeo Marine S … More tation, Okayama Univ. The ascidian, Ciona intestinalis, widely used esperimental material in the field of diology and another ascidian species of which oocyte is transparent and the cell has concentrated vanadate were tried to keep in the aquarium to supply them to the scientist in the world. The establishment of the strain in these species was also conducted in the Mukaijima marine Laboratory, Hiroshima University. Gene Analysis : Using genomic DNA obtained from the sperm of sea-lilies, the gene library was made. Six genes for spicle formation, sm-50 and Hoxgenes were used as probes and gene homologues of these genes in sea-lilies were isolated usingthe gene library of the sea-lilies in the MMBS.In the Mie University, three types of actin genes were isolated from sagitta rearing in the laboratory and identified its expression portion in the gene. Under the results the phylogenic difference of sagitta and othe animal phyla was investigated. The identity and difference of gene in the ascidian species Ciona intestinalis among the habitats for example Quintao in China and Onagawa in Japan with the investigation of polymorphism of mRNA in the TTT. Less

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (100 results)

All Other

All Publications (100 results)

  • [Publications] Inaba,K.: "Identification of tubulins associated with the 950kDa protease from salmon sperm." Biomed.Res.17. 87-93 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mukai,T.: "Genetic relationships of the genus Tridentiger (pices,Gobiidae) based on allozyme polymorphism." Zool.Sci.13. 175-183 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minamikawa,S: "Acquisition initiation and maintenance of sperm motility in the shark,Triakis scyllia." Comp.Biochem.Physiol.113. 387-392 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Deguchi,R.,: "Extracellular Ca2+release from inositol 1,4,5-triphosphate-sensitive stores function at fertilization in oocytes of the marine vivalveMytilus edulis" Development. 122. 3651-3660 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oda,S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring,Clupea Pallasii." Develop.Growth & Differ.37. 257-261 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa,M.: "Signal transduction mechanisms for sperm m otility." Zool.Sci.12. 5 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo,T: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis." Biol.Bull.189. 330-339 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takai,H.: "Change in intracellular K+concentration caused by external osmolality change regulates sperm motility of marine and fresh water teleosts." J.Cell Sci.108. 1175-1181 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Buerano,C.C.: "Vitellogenins of Oreochromis niloticus : Identification,isolation and biochemical and immunological characterizations." J.Exp.Zool.273. 59-69 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba,K: "Identification of a 10 S trypsin-like protease that cross-reacts with anti-proteasome antibody in sea urchin egg jelly." Arch.Biochem.Biophys.319. 177-184 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanimoto,S.: "Evidence that potassium flux and increase in intracellular calcium mediate the initiation of sperm motility in salmonid fishes." Mol.Reprod.Develop.39. 409-414 (19954)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida,M.: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemotaxis in the ascidian,Ciona savignyi" Develop.Growth & Differ.36. 589-595 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa,M.: "Cell signalling mechanisms for sperm motility" Zool.Sci.11. 647-662 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,Z.X.: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon." J.Biochem.115. 552-556 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin,Z.X.: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta." J.Biochem,. 115. 885-890 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sensui,N.: "Oocyte maturation and furrow formation in an unfertilized egg by fusion with a fertilized egg or blastomeres in the ascidian.Ascidia sydneiensis divisa" Develop.Growth & Differ.36. 373-380 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K., K.Ohkawa, et al: "Identification of tubulins associatedwith the 950 kDa protease from salmon sperm." Biomed.Res.17. 87-93 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mukai, T., T.Sato, et al.: "Genetic relationships of the genus Tridentiger (pices, Gobiidae) based on allozyme polymorphism." Zool.Sci.13. 175-183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Minamikawa, S.and M.Morisawa: "Acquisition, initiation and maintenance of sperm motility in the shark, Triakis scyllia." Comp.Biochem.Physiol.113(4). 387-392 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Deguchi, R., K.Osanai, et al: "Extracellular Ca2+release from inositol 1,4,5-triphosphate-sensitive stores function at fertilization in oocytes of the marine vivalve Mytilus edulis." Development. 122. 3651-3660 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Oda, S., Y.Lgarashi, et al: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea Pallasii." Develop.Growth & Differ. 37. 257-261 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa, M.: "Signal transduction mechanisms for sperm m otility." Zool.Sci.12. 5 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Togo, T., K.Osanai, et al: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis." Biol.Bull.189. 330-339 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takai, H.and M.Morisawa: "Change in intracellular K+concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts." J.Cell Sci.108. 1175-1181 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Buerana, C.C., K.Inaba, et al: "Vitellogenins of Oreochromis niloticus : ldentification, isolation and biochemical and immunological characterizations." J.Exp.Zool.273(in press). 569 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inaba, K.and M.Morisawa: "Identification of a 10S trypsinlike protease that cross-reacts with anti-proteasome antibody in sea urchin eggjelly." Arch.Biochem.Biophys.319. 177-184 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tanimoto, S., T.Nakazawa, et al: "Evidence that potassium flux and increase in intracellular calcium mediate the initiation of sperm motility in salmonid fishes." Mol.Reprod.Develop.39. 40414 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yoshida, M., K.Inada, et al: "Calcium and cyclic AMP mediate sperm activation, but Ca alone contributes sperm chemotaxis in the ascidian, Ciona Savignyi." Develop.Growth & Differ.36. 589-595 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morisawa, M.: "Cell signalling mechanisms for sperm motility." Zool.Sci.11. 647-662 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin, Z.X., T.Nakajima, et al: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon." J.Biochem.115. 552-556 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Jin, Z.X., K.Inaba, et al: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phoshoprotein of Oncorhynchus keta." J.Biochem. 115. 885-890 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sensui, N., M.Ishikawa, et al: "Oocyte maturation and furrow formation in an unfertilized egg by fusion with a fertilized egg or blastomeres in the ascidian, Ascidia sydneiensis divisa." Develop.Growth & Differ.36. 373-380 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Deguchi,R.et al.: "Extraceilular Ca^<2+> release from inositol 1,4,5-triphoaphote-sensition sbora function of ferle lijation in oocytes of the marine vivalue Mytilus eilulis" Development. 12. 3651-3660 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mukai,T.et al.: "Genetic relationships of the genus Tridenteger(pices,Gobudae)leaud on alloenzyme paly mernlusm" Zaological Science. 13. 175-183 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minamikawa,S.et al.: "Acquisition,initoation and maintenance of spmor matility in the shark Trinkis seyiba" Conporatue Biodumidtry ad plupiology. 113. 378-392 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sensui,N.et al.: "Effect of Ca^<2+> on defermatcer,polarbody extrusium ad pronucleds formation in the egg of the asciidaim Cooma savignye" Development,Grouth & Differntiation. 38. 341-350 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inaba,K.et al.: "Identification of tubalins associated with tl groKDA protease from salenum sperm" Biomedical Research. 17. 87-93 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Togo,T.et al.: "Aminopeptidase-like protease released from oocyted nappresses acroione reactievr sperm and established poly spermy block in oocyte of the mussel" Development Biology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nose,Y.et al.: "Specific increase in the number of vanadium-accumulating blood cells by some ionophores and inhibitors of proton-ATPases in the ascidian,Ascidia sydneiensis samea." Zool.Sci.14(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Uvama.T.et al.: "Finding of the same antigen in the polychaeta,Pseudopotamilla occelata,as those in the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 43-47 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kanda,T.et al.: "Identification and localization of a vanadium-associated protein from the vanadium-rich ascidian,Ascidia sydneiensis samea." Zool.Sci.14. 37-42 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi,M.et al.: "Rapid increases in number of vanadocytes in the vanadium-rich ascidian,Ascidia sydneiensis samea,upon treatment of live animals with NH_4Cl." J.Exp.Zool.275. 1-7 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Michibata,H.: "The mechanism of accumulation of vanadium by ascidians:Some progress towards an understanding of this unusual phenomenon." Zool.Sci.13. 489-502 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 道端 齊.: "ホヤのバナジウム濃縮に携わる特異な細胞、バナドサイトの謎を探る." 生物物理. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Okada: "Secretory function of the test cell in leaval tumic formation in the ascidian Ciona intestinalis" Zoological Science. 13. 253-261 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Katayama: "Phylogenetic relationships of turbellarian orders inferred from 18S rDNA sequences" Zoological Science. 13. 747-756 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Yasuda et al.: "Phylogenic status of Chaetognaths,as deduced from actin seguences" Zoological Science. 13. 39 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Goto et al.: "Regeneration of the mechanocensory organ of the arrowaorm paruspadella gotoi" Zoological Science. 13. 82 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 安田映津子 等: "毛顎動物ヤムシ神経系観察のための〓銀染色法" 三重大学教育学部研究紀要. 47. 45-49 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Goto et al.: "Growth and reprodaction of the buitluc arrourworm praspadella gotoi(Chaetognatha)in lalooratony cultuve" Invertebrate Reproduction & Development. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Minokawa,Y.Hamaguchi and S.Amemiya: "Skeletogenic potential of induced secondary mesenchyme cells derived from the presumptive ectoderm in echinoid" Development,Genes and Evolution. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Amemiya and E.Arakawa: "Variation of cleavage pattern permitting normal development in a sand dollar,Peronell japonica" Development,Genes and Evolution. 206. 125-135 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Amemiya: "Complete regulation of development throughout metamorphosis of sea urchin embryos devoid of macromeres" Development,Growth and Differentiation. 38. 465-476 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 雨宮昭南: "棘皮動物はいかにつくられたか" 科学. 66. 306-311 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 雨宮昭南: "後生動物のガラス骨格と炭酸カルシウム骨格" 海洋. 28. 643-648 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 東中川徹・梶原薫・雨宮昭南: "ウニ胚骨片マトリックス遺伝子" 海洋. 28. 654-658 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishigaki T.et al.: "Structure and function of asferosaps,Sperm-activating peptrdes from the jelly coat of Shorfish eggs" Zygete. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shogomori,H.: "Association of the mejor genalioside in sea unchin eggs with yock bipopnotions" Glycobiology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 森沢正昭 他: "発生生物学" 朝倉書店, 182 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Michibata,H.& Kanamori,K.: "Selective accumulation of vanadium by ascidians from seawater.In “Adv.Environm.Sci.& Technol."" J.John Wiley & Sons,Inc.,edit.Nriagu(in press), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 石原勝利(編): "動物発生段階図譜" 共立出版, 348 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Buerano, C. C.: "Vitellogenins of Oreochromis niloticus: Identification, isolation and biochemical and immunological characterizations." J. Exp. Zool.273. 59-69 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Inaba, K.: "Identification of a 10 S trypsin-like protease that cross-reacts with anti-proteasome antibody in sea urchin egg jelly." Arch. Biochem. Biophys.319. 177-184 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Inaba, K.: "Identification of tubulins associated with the 950 kDa protease from salmon sperm." Biomed. Res.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Oda, S.: "Sperm-activating proteins from unfertilized eggs of the Pacific herring, Clupea Pallasii." Develop. Growth & Differ.37. 257-261 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takai, H.: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts." J. Cell Sci.108. 1175-1181 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Togo, T.: "Existence of three mechanisms for blocking polyspermy in oocytes of the mussel Mytilus edulis." Biol. Bull.189. 330-339 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Minamikawa, S.: "Acquisition, initiation and maintenance of sperm motility in the shark, Triakis scyllia." Comp. Biochem. Physiol.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Akita, N.: "Hemocytes Release Phenoloxidase upon Contact Reaction, an Allogeneic Interaction, in the Ascidian Halocynthia roretzi" Cell Struc. Func.20. 81-87 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Katayama, T.: "Phylogenetic position of the dicyemid mesozoa interred from 18S rDNA sequences." Biol. Bull.189. 81-90 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Okada, T.: "Secretory function of the test cell in larval tunic formation in the ascidian Ciona intestinalis: An immunoelectron microscopic study." Zool. Sci.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaneko, A.: "Localization, with monoclonal antibodies and detection of autonomous fluorescence, of blood cells" Zool. Sci.12. 733-739 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wuchiyama, J.: "Classification, based on autonomous fluorescence, of the blood cells of several ascidians that contain high levels of vanadium." Acta Zool.76. 51-55 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hayashi, M.: "Rapid increases in number of vanadocytes in the vanadium-rich ascidian, Ascidia sydneiensis samea, upon treatmentof live animals with NH4Cl." J. Exp. Zool.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Michibata, H.: "The mechanisms of accumulation of metals, especially high levels of vanadium, by ascidians." Zool. Sci.(in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Goto, T.: "Occurrence of Spadella cephaloptera in a year at Ischia Island(Gulf of Naples)." P. S. Z. N. I. : Marine Ecology. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] Casanova, J. -P.: "Emergence of limb-like appendages from fins in chaetognaths." Acad. Sci. Paris. (in press).

    • Related Report
      1995 Annual Research Report
  • [Publications] M. Tsushima: "Comparative biochemical studies of carotenoids in sea urchins--III." Comp. Biochem. Physiol.110B. 719-723 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Yoshida: "Calcium and cyclic AMP mediate sperm activation,but Ca alone contributes sperm chemotaxis in the ascidian,Ciona savignyi" Develop.Growth & Differ.36. 589-595 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Tanimoto: "Evidence that potassium flux and increase in intracellular calcium mediate the initiation of sperm motility in salmonid fishes" Mol.Reprod.Develp.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Z.X.Jin: "Monoclonal antibodies against the protein complex that contains the flagellar movement-initiating phosphoprotein of Oncorhynchus keta" J.Biochem,. 115. 885-890 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Morisawa: "Cell signalling mechanisms for sperm motility" Zool.Sci.11. 647-662 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Z.X.Jin: "Isolation and properties of a protein complex that contains flagellar movement-initiating phosphoprotein from testes of a white salmon" J.Biochem.115. 552-556 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Sensui: "Oocyte maturation and furrow formation in an unfertilized egg by fusion with a fertilized egg or blastomeres in the ascidian,Ascidia sydneiensis divisa" Develop.Growth & Differ. 36. 373-380 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Takai: "Change in intracellular K^+ concentration caused by external osmolality change regulates sperm motility of marine and freshwater teleosts" J.Cell Sci.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yamamoto: "Synthesis of Ganglioside M5 from Sea Urchin Egg" Tetrahed.Lett.35. 2701-2704 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] F.J.Longo: "Ultrastructural Localization of Acrosome Reaction-Inducing Substance (ARIS)on Sperm of the Starfish,Asterias amurensis" Mol.Rep.Dev.(in press). (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Akita: "Hemocvtes Release Phenoloxidase upon Contact Reaction,an Allogeneic Interaction,in the Ascidian Halocynthia roretzi" Cell Struc.Func.20. 81-87 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Chiba: "A Periodic Network of G Protein βγ Subunit Coexisting with Cytokeratin Filament in Starfish Oocytes" Dev.Biol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Hoshi: "Glycosidases,Proteases and Ascidian Fertilization" Seminars Dev.Biol.5. 201-208 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Hoshi: "Egg-Jelly Signal Molecules for Triggering the Acrosome Reaction in Starfish Spermatozoa" Internat.J.Dev.Biol.38. 107-174 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of the japanese holothurian Eupentacta chronhjelmi." Canad.J.Zool.72. 387-396 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.A.B.Catalan: "Annual reproductive cycle of two Japanese species of sipunculans Siphonosoma cumanense(Sipunculidae)and Phascolosoma scolops(Phascolosomatidae)." Pacific Sci.48. 145-157 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kanamori,K.: "Raman specroscopic study of the vanadium and sulfate in blood cell homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Wuchiyama,J.: "Classification,based on autonomous fluorescence,of the blood cell of several ascidians that contain high levels of vanadium." Acta Zool.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Uyama,T.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian,Ascidia sydneiensis samea." J.Exp.Zool.270. 148-154 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hikosaka,A: "Short upstream sequences associated with the muscle-specific expression of an actin gene in ascidian embryos." Developmental Biology. 166. 763-769 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsushima,M.: "Comparative biochemical studies of carotenoids in sea-urchins-III." Comp.Biochem.Physiol.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Amemiya,S.: "First fossil record of the family Phormosomatidae(Echi-nothurioida:Echinoidea)from the early Miocene" Zoological Science. 11. 313-317 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoh,Noriyuki: "Developmental Biology of Ascidians" Cambridge University Press, 234 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi