• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Design of peptide-containing chelate metal complexes for recognition of specific base sequences in DNA

Research Project

Project/Area Number 06640733
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Inorganic chemistry
Research InstitutionChuo University

Principal Investigator

CHIKIRA Makoto  Chuo Univ.Scien.& Eng.Prof., 理工学部, 教授 (70006328)

Co-Investigator(Kenkyū-buntansha) ISHIZUKA Morio  Chuo Univ.Scien.& Eng.Prof., 理工学部, 教授 (50168241)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordsspecific recognition / DNA-base sequences / peptide-containing metal complexes / DNA-fiber ESR / 2D-NMR / phenanthroline / Schiff-base / water-soluble porphyrin / DNA / 塩基配列特異性 / ぺプチド複合錯体 / DNAファイバー / ESR / フェナントロリン錯体 / 大環状ポリアミン / ペプチド複合錯体 / シッフ塩基錯体
Research Abstract

In order to evaluate the function of amino acid residues in the stereospecific binding of peptide-containing chelate complexes on DNA,the binding structures of various amino acid and peptide complexes of Cu (II) were investigated by DNA-fiber ESR spectroscopy and it was revealed that the orientation of the coordination plane of the complexes could be regulated by an appropriate choice of the amino acid and the sequence in the peptides. It was also found by means of acrylamide gel electrophoresis that some histidine-containing peptide complexes recognize differently the DNA base sequence from other peptide complexes in the DNA scission reaction with hydrogen peroxide. The research on the structure or the binding constant for various Cu (II) complexes of cationic water soluble porphyrins and Schiff bases were also published.
The investigation was further extended over the phenanthroline Cu (II) complexes. Various 2,9-or 5-substituted-1,10-phenanthroline, that are the intermediates for the combined peptide-chelate ligands, were synthesized and the function of the Cu (II) complexes in the interaction with DNA were assayd by the DNA-fiber ESR spectroscopy and by an agarose gel electrophoresis of the reaction products of phage DNA with hydrogen peroxide. It was revealed that the cleaving is active for the complexes randomly orienting on the DNA and for the ligands with more electron-withdrawing substituents such as NO2 or Cl. It was proposed that the non-intercalated species activate hydrogen peroxide and that the pKa value of the coordinated water molecules affect the reactivity. The binding structure of mixed chelate complex of 1,10-phenanthroline and leucine with Pt (II) on self-compliment oligonucleotide, GCGCAATTGCGC,was investigated by 2D-NMR and was found that this complex intercalate from the major groove at the AT base pair.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 千喜良 誠: "DNA塩基配列特異性を認識するペプチド金属錯体の分子設計" 中央大学理工学研究所年報. 2. 43-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 千喜良 誠: "DNA塩基配列特異性を認識するペプチド金属錯体の分子設計" 中央大学理工学研究所年報. 3(印刷中). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 千喜良 誠: "塩基性アミノ酸銅(II)錯体とDNAとの相互作用における不斉の効果" 中央大学理工学研究所論文集. 2(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] W.Harada: "How Amino Acid Control the Binding of Copper (II) Ion to DNA ; The Role of Hydroxyl Group of Serine and Threonine in Fixing the Orientation of the Complexes" J.Inorg.Biochem.64. 273-285 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "How Amino Acid Control the Binding of Copper (II) Ions to DNA ; (II) Efect of Basic Amino Acid Residues and the Chilarity on the Orientation of the Complexes" J.Inorg.Biochem.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Electron Spin Resonance Study of the Binding Structures of Cationic Water-soluble Metlloporphyrins on HIghly Oriented Deoxyribonucleic Acid Fibres" J.Chem.Soc.,Dalton Transaction. 1325-1331 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Orientation of Copper (II) Complexes of 1,10-Phenanthroline and its Derivatives on DNA-fibers" Magnetic Resonance in Medicine. 6. 214-215 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sato: "Stereospecific Binding of Chemically Modified Salen-type Schiff-base complexes of Copper (II) with DNA [salen=bis (salicylidene) ethylenediamine]" J.Chem.Soc.,Chem.Commun.625-626 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Tanaka: "Synthesis of New Cationic Schiff Base Complexes of Copper (II) and Their Selective Binding with DNA" Bull.chem.Soc.Jpn.(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Design of peptide-containing chelate metal complexes for specific recognition of base sequences in DNA" Annual.Rep.Inst.Sci.Eng.Chuo Univ.2. 43-47 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Design of peptide-containing chelate metal complexes for specific recognition of base sequences in DNA" Annual.Rep.Inst.Sci.Eng.Chuo Univ.3 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Effect of Chirality of Basic Amino Acid on the Interaction of the copper (II) complex with DNA" Bull.Rep.Inst.Sci.Eng.Chuo Univ.2, (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] W.Harada: "How Amino Acid Control the Binding of Copper (II) Ion to DNA ; (I)The Role of Hydroxvl Group of Serine and Threonine in Fixing the Orientation of the Complexes." J.Inorg.Biochem.64 (4). 273-285 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "How Amino Acids Control the Binding of Copper (II) Ions to DNA (II) ; Effect of Basic Amino Acid Residues and the Chirality on the Orientation of the Complexes" J.Inorg.Biochem.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Electron Spin Resonance Study of the Binding Structures of Cationic Water-soluble Metalloporphyrins on Highly Oriented Deoxyribonucleic Acid Fibers." J.Chem.Soc.Dalton Transaction. 1325-1331 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira: "Orientations of Copper (II) Complexes of 1,10-Phenanthroline and its Derivatives on DNA-fibers." Magnetic Resonance in Medicine. 6. 214-215 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Sato: "Stereospcific Binding of Chemically Modified Salen-type Schiff base Complexes of Copper (II) with DNA [salen=bis (salicylidene)-ethylenediamine]." J.Chem.Soc., Chem.Commun.625-626 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Tanaka: "Synthesis of New Cationic Schiff Base Complexes of Copper (II) and Their Selective Binding with DNA." Bull.Chem.Soc.Jpn.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] M.Chikira,他7名: "Electron Spin Resonance Study of the Binding Structures of・・・・・" J.Chem.Soc.,Dalton Trans.1325-1331 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Chikira,他4名: "Orientation of Copper(II) Complexes of 1,10-Phenanthroline・・・・" Mag.Resonance in Medicine. 6. 214-215 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] M.Chikira,他4名: "Binding Structure and Reaction of Amino Acid and Peptide・・・・" J.Inorg.Biochem.59. 170-170 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 千喜良誠・石塚盛雄: "DNA塩基配列特異性を認識するペプチド金属錯体の分子設計" 中央大学理工研究所年報. 2. 43-46 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] W.Harada,他5名: "How Amino Acid Control the Binding of Cu(II)ions to DNA・・・・" J.Inorg.Biochem.61(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Sato,M.Chikira,Y.Fuji,A.Komatsu: "Stereospecific Binding of Chemically Modified Salen-type Schiff Base Compexes of Copper(II) with DNA [salen=bis(salicylidene)ethylene diamine]" J.Chem.Soc.,Chem.Commun.625-626 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Chikira,Y.Tomizawa,D.Fukia T.Sugizaki,M.Palaniandavar: "Orientations of Copper(II)complexes of 1,10-Phenunthroline" Mag.Resonance Med.6. 214-215 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Chikira,他7名: "ESR Stcly of Binding Structures of Cationic Water-soluble Metolls-porphyrins on the Highly Oriented DNA-fibres" J.Chem.Soc.,Dalton Transaction. (in press). (1995)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi