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Deveopment of PIXE for Small Biological Samples and Its Application to Study for Mechanism of Accumulation of Trace Elements

Research Project

Project/Area Number 06640773
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分離・精製・検出法
Research InstitutionAkita University

Principal Investigator

IWATA Yoshihiro  Department of Chemistry, College of Education, Akita University Assistant Professor, 教育学部, 助教授 (70213296)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1995: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPIXE / nondestructive analysis / biologial sample / small sample / diatom / multielement analysis / algae / accumulation / 甲殻類 / 薄膜
Research Abstract

A thin support film is indispensable to make irradiation targets of a small amount of biological material for trace anlyasis by PIXE.We developed a simple technique for the preparation of high quality support film. The thickness of this polyvinylformarl (PVF) film was 0.1 mm with good homogeneity. The continuous X-ray making background of spectrum was <1/20 that of conventional 10mm Maylar film. We calculated theoretical detection limits and also observed <1ng detection limits for many kinds of elements by 3MeV protons from a cyclotron. (Reference 1,2,4) The PVF film was applied for the support film for <200mug of biological materials. The reliability of the PIXE analysis using the PVF film was evaluated by analysis for biological standart materials. The elemental abundance in the small Crustacea such as Artemia salina was surveyed by the PVF PIXE.artemia salina was bred in artificial sea water and the characteristic of elemental abundance as a function of size and breeding day was described. (Reference 3) Diatom such as Pheodictylum sp. from Pacific Ocean was also bred in artificial sea water and the elemental abundance in the small Pheodictylum sp.was surveyed by PIXE.Accumulation behavior of trace elements especially for zinc was observed by multielemental analysis by PIXE.
Many kinds of analytical methods for determination of inorganic elements are available, however, most of them are not suitable for in sutu analysis of biological materials. Biological and environmental science ofter require simultaneous determination of trace elements inliquid and gel samples. However, conventional analytical methodns require pretreatment of samples such as freeze drying, wet digestion, etc. By constant, vertical type in-air PIXE is powerful tool for in situ analysis because of no pretreatment. We have planned to install a new vertical type in-air PIXE and discussed the detection limits for many kinds of elements in liquid and thin film target. (Reference 5)

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] K.Ishii: "Detection Limit for a Very Thin Backing Film" International Journal of PIXE. 4. 1-7 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ishii: "Compton Tail Background due to Sodium Element in Biological Samples" International Journal of PIXE. 4. 137-145 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩田吉弘: "PIXE法による種々の微小生体試料中の微量元素の定量" NMCC共同利用研究成果報文集. 2. 87-90 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 岩田吉弘: "体液および組織中微量元素含有量とその測定法 -粒子励起X線分光法-" 日本臨床. 54. 221-227 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Iwasaki: "In-Air System of Vertical Beam Type at Tohoku Cyclotron Radioisotope Center" International Journal of PIXE. 5(印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.ISHII,H.ORIHARA,Y.IWATA and K.BESSHO: "Detection Limit for a Very Thin Baking Film" International Journal of PIXE. 4. 1-7 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.ISHII,H.ORIHARA,S.IWASAKI,K.SERA,S.FUTATSUGAWA and Y.IWATA: "Compton Tail Background due to Sodium Element in Biological Samples" International Journal of PIXE. 4. 137-145 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.IWATA,A.MURA and K.SERA: "Determination of Trace Elements in Several Kinds of Small Amount of Biological Materials by PIXE Analysis" JAPAN RADIOISOTOPE ASSOCIATION NISHINA MEMORIAL CYCLOTRON CENTER ANNUAL REPORT. 2. 87-90 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.IWATA: "Particle Induced X-ray Emission (PIXE)" NIHONRINSOU. 54. 221-227 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.IWASAKI,K.ISHII,K.YOSHIZAKI,H.FUKUDA,K.MUROZONO,J.INOUE,M.KITAMURA,H.YOKOTA,Y.IWATA,H.ORIHARA: "In-Air PIXE System of Vertical Beam Type at Tohoku Cyclotron Radioisotope Center" International Journal of PIXE. 5 (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Ishii: "Detection Limit for a Very Thin Backing Film" International Journal of PIXE. 4. 1-7 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ishii: "Compton Tail Background due to Sodium Element in Biological Samples" International Journal of PIXE. 4. 137-145 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岩田 吉弘: "体液および組織中微量元素含有量とその測定法‐粒子励起X線分光法‐" 日本臨牀. 54. 221-227 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] S.Iwasaki: "In‐Air System of Vertical Beam Type at Tohoku Cyclotron Radioisotope Center" International Journal of PIXE. 5(印刷中). (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Ishii: "Detection limit for a Very Thin Backing Film" International Journal of PIXE. 4. 1-7 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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