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DEVELOPMENT OF AN ELECTROCHEMICAL MEASUREMENT METHODS ABOUT BIOFUNCTIONAL MATERIALS AND THEIR APPLICATION TO NONAQUEOUS SOLUTION SYSTEMS

Research Project

Project/Area Number 06640778
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 分離・精製・検出法
Research InstitutionShinshu University

Principal Investigator

NAKAMURA Toshio  FACULTY OF SCIENCE,DEPARTMENT OF CHEMISTRY,ASSOCIATE PROFESSOR, 理学部, 助教授 (00020678)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBiofunctional materials / Heme protain / Methylviologen / Polyacrylamide / Cyclic voltammetry / Cyclohexanol / NADH / Oxygen / シクロヘキサノール / 酸素 / 生体関連物質 / 酵素・補酵素 / 酸化還元反応 / 電子移動反応 / 高分子膜電極 / 水溶媒ボルタンメトリー / ヘキサノールの酸化 / 色素蛋白質
Research Abstract

Studies for getting basic data concerning to redox and electron transfer about biofunctional materials and for developing sensors for use both in detection of those and in tracing their activities were carried out. Biofunctional materials, which are generally difficuit to exchange electrons between those and an electrode, were contained in fairly a thin film of hydrophilic polymer, polyacrylamide (PAA)coated on a platinum disk electrode. A cell was constructed with the electrode and an acentonitrile(AN) solution to investigate electrochemical functions. 1)A PAA and methylviologen were dissolved with water on a platinum disk and dried in a sil-ica gel descicator. From the cyclic voltammetry (CV) the cell constructed with the electrode was confirmed to share a necessary condition for carring out the research. 2)The platinum disk electrodes coated with a PAA membrane containing a heme protein (catalase, bleomycin, hemoglobin, or cytochrome c) were constructed. It was found from the electrode that CV behavior about the redox of oxygen were different each other according to a containing protain. It should be nescessary to investigate the effect against the fedox behavior of oxygen by the difference of protains and the charge of the iron. 3)The platinum disk electrodes coated with a PAA membrane con-taining some functional materials(NADH,NAD,PQQ,diaphrase)were constructed. It was found from the electrode that redox peak currents were proportional to the con-centration of the cyclohexanol in AN.4)The PAA in gel type used in the experiment as a matrix for fixing biofunctional materials close to platinum disk was investi-gated to elucidate electrochemical basic characteristics.(Pacifichem'95, part 1, ANYL 193(1995). (Honolulu, Dec 17-22))Informations about redox behavior of a cho-lesterol and an oxygen monoxide were also obtained by the platinum electrode coated with a PAA containing cobalt phthalocyanine or hemoglobine.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 中村俊夫,小川原鉄志 伊豆津公佑: "フタロシアニン金属錯体を化学結合した高分子を感応膜とする非水溶液用陰イオンセンサーの電位応答機構の研究" Rev.of Polarogr.40(3). 35- (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Izutu,T.Nakamura T.Arai,M.Ohmaki: "Some recent studies on the use of electrochemical sensors in nonaqueous solution chemistry" Electroanalysis. 7(9). 884-888 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 中村俊夫,西川裕志 伊豆津公佑: "高分子被覆膜電極に固定した生体機能物質の非水系ボルタンメトリー挙動" Rev.of Polarogr.41(3). 55- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura,C.Hayashi T.Ogawara: "Potentiometric response properties of sensor membrane based on cobalt phthalocyanine conjugate-polymer in nonaqueous solutions" BCSJ. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura,H.Nishikawa: "Development of the method of an electrochemical measurement about biofunctional materials and its application to nonaqueous solution systems"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Izutsu, T.Nakamura, T.Arai, M.Ohmaki: "Some recent studies on the use of electrochemical sensors in nonaqueous solution chemistry" Electroanalysis. 7(9). 884-888 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura, C.Hayashi, T.Ogawa: "Potentiometric response properties of sensor membrane based on cobalt phthalocyanine conjugate-polymer in nonaqueous solutions" BCSJ. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura, H.Nishikawa, K.Izutsu: "Development of the method of an electrochemical measurement about biofunctional materials and its application to nonaqueous solution systems" (to be appeared).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura, T.Ogawara, K.Izutsu: "Study of the potentiometric respose mechanism of the sencor membrane based on the cobalt phthalocyanine conjugated-polymer" Rev. of Polarogr. 40. 35 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Nakamura, H.Nishikawa, K.Izutsu: "Voltammetric behaviors of biofunctional materials immobilized in a polymer coated on a platinum disk electrode" Rev. of Polarogr. 41(3). 55 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Izutsu,T.Nakamura,A.Arai,M.Ohmaki: "Some recent studies on the use of electrochemical sensors in nonaqueous solution chemistry" Electroanalysis. 7(9). 884-888 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nakamura,C.Hayashi,T.Ogawara: "Potentiometric response properties of sensor membrane based on cobalt phtalocyanine conjugate-polymer in nonaqueous solutions" Bulletin of the chemical sciety of Japan. (印刷中).

    • Related Report
      1995 Annual Research Report
  • [Publications] 中村俊夫,西川裕志,伊豆津公佑: "高分子被覆膜電極に固定した生体機能物質の非水系ボルタンメトリー挙動" Rev. of Polarogr.41(3). 55- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nakamura,H.Nishikawa: "Development of the method of an electrochemical measurement about biofunctional materials and its application to nonaqueous solution systems" (投稿予定).

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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