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Construction of cyanobacterial mutant strains which lack a specific subunit of photo system I and the characterization of their photosynthetic properties.

Research Project

Project/Area Number 06640832
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理
Research InstitutionSaitama University

Principal Investigator

NAKAMOTO Hitoshi  SAITAMA UNIV,DEPT.OF BIOCHEMISTRY AND MOLECULAR BIOLOGY,ASSOCIATE PROFESSOR, 理学部, 助教授 (30192678)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsPhotosystem I / Synechocystis / Targeted mutagenesis / PsaK / 光合成 / Synechocystis sp. / ランソウ
Research Abstract

Photosystem I (PSI) is a multisubunit pigment-protein complex that consists of more than 10 different polypeptides (from PsaA to PsaM) and catalyzes light-driven electron transfer from cytochrome c_6/plastocyanin to ferredoxin. In this study, we have generated the first PsaK-deficient mutant strains of Synechocystis sp.PCC 6803 byogene-targeting in order to identify roles of PsaK in PSI function and organization. Nothing is known about the function of PsaK.DNA fragment containing psaK was amplified from the Synechocystis sp.PCC 6803 genomic DNA by PCR with using nucleotide primers made according the sequence information for psaK which was obtained from Cyanobase, the database on the genome of Synechocystis sp.PCC 6803. The PCR fragment was then cloned into pGEM-T vector. A mutant strain lacking PsaK was generated by transforming the wild type cells with cloned DNA in which psaK gene was interrupted by a gene conferring resistance to kanamycin. Northern blot analysis and SDS polyacrylamide gel electrophoresis revealed that the mutant strain lacked both the transcript and translation products of psaK.The amount of chlorophyll and phycobilisome per cell basis was reduced, suggesting the involvement of PsaK with the binding of those pigments. However, photoautotrophic growth and photosynthetic characteristics of the mutant strain were similar to those of the wild type.
The mutational studies have shown that mutants lacking one (or two) of the accessory subunits of PSI photosynthesize as actively as the wild type. The accessory subunits of PSI do not bind any redox-active cnfactors like PsaA,PsaB and PsaC.In order to find physiological importance of these accessory subunit, we compared phenotypes of the mutant strains with that of the wild type under various stress conditions. We have obtained some evidences which demonstrate that PsaD and PsaE are important for the protection of cyanobacterial cells from photoinhibition and heat-damage.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 仲本準: "Targeted Inactivation of the Gene psaI Encoding a Subunit of Photosystem I of the Cyanobacterium Synechocystis sp.PCC 6803" PLANT AND CELL PHYSIOLOGY. 36. 1579-1587 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 仲本準: "Some studies with mutant strains in which specific genes encoding subunits of photosystem I were inactivated" PHOTOSYNTHESIS : FROM LIGHT TO BIOSPHERE (P.MATHIS,ED.) KLUWER ACADEMIC PUBLISHERS. 3. 513-516 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 仲本準: "Cloning,characterization and transcriptional studies of ferredoxin genes from the mesophilic Cyanobacterium Synechocystis sp.PCC 6803 and the thermophilic cyanobacterium Synechococcus vulcanus." PHYSIOLOGIA PLANTARUM. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Nakamoto: "Targeted inactivation of the gene psaI encoding a subunit of photosystem I of the cyanobacterium Synechocystis sp.PCC 6803" Plant & Cell Physiology. Vol.36. 1579-1587 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Nakamoto: "Some studies with mutant strains in which specific genes encoding subunits of photosystem I were inactivated" Photosynthesis : from Light to Biosphere (P.Mathis ed.), Kluwer Academic Publishers. Vol.III. 513-516 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hitoshi Nakamoto and Toyoko Suzuki: "Cloning, characterization and transcriptional studies of ferredoxin genes from the mesophilic cyanobacterium Synechocystis sp.PCC 6803 and thermophilic cyano-bacterium Synechococcus vulcanus" Physiologia Plantarum. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] NAKAMOTO, HITOSHI: "TARGETED INACTIVATION OF THE GENE psaI ENCODING A SUBUNIT OF PHOTOSYSTEM I OF THE CYANOBACTERIUM Synechocystis sp. PCC 6803" PLANT AND CELL PHYSIOLOGY. 36. 1579-1587 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] NAKAMOTO, HITOSHI: "SOME STUDIES WITH MUTANT STRAINS IN WHICH SPECIFIC GENES ENCODING SUBUNITS OF PHOTOSYSTEM I WERE INACTIVATED." PHOTOSYNTHESIS: FROM LIGHT TO BIOSPHERE (Proceedings of the Xth Internatinal Photosynthesis Congress). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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