| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Research Abstract |
In order to elucidate the regulatory mechanisms of plant differentiation by plant growth regulators, transcription regulatory factors were investigated in carrot suspension cultured cells. In this research, myb related genes, one of transcription regulatory factors known as proto-oncogene in animal cells, were isolated from subtracted cDNA libraries of carrot suspension cultured cells induced for anthocyanin synthesis. Five clones of myb related genes (DCmyb8, DCmyb10, DCmyb12, DCmyb14 and DCmyb35) were isolated and their nucleotide sequences were determined. DCmyb10 and DCmyb12 were highly similar (98% similarity in nucleotide sequences), but the others had less identity than 55% in nucleotide sequences or 70% in translated amino acid sequences. Using specific nucleotide sequences of each myb related genes as probes, expression patterns of these genes were analyzed in carrot cultured cells under dedifferentiated growth, under differentiated states inducing for anthocyanin synthesis or embryogenesis, and in an anthocyanin-synthesizing mutant cell line or an embryogenic cell line of carrot callus cultures. Both DCmyb8 and DCmyb10 (12) expressed in anthocyanin-synthesizing cells under differentiated states. The expression of DCmyb10 was observed in a mutant cell line synthesizing anthocyanin, but that of DCmyb8 was not in it. DCmyb14 and DCmyb35 expressed in the cells under embryogenesis, especially DCmyb35 did in the cells of globular stage. The results obtained here suggested that myb genes isolated here expressed in cells under different differentiation states in different manners, and that the individual genes might regulate the expression of genes involved in different differentiation process.
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