| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Research Abstract |
Flash-induced optical kinetics at room temperature of cytochrome (Cyt) c_<551> and an Fe-S center (CF_A/CF_B) bound to a purified reaction center (RC) complex from the green sulfur photosynthetic bacterium Chlorobium tepidum were studied. At 551 nm, the flash-induced absorbance change decayd with a t_<1/2> of several hundred ms, and the decay was accelerated by 1-methoxy-5-methylphenazinium methyl sulfate (mPMS). In the blue region, the absorbance change was composed of mPMS-dependent (Cyt) and mPMS-independent component (CF_A/CF_B) which decayd with a t_<1/2> of -400-650 ms. Decay of the latter was effectively accelerated by benzyl viologen (Em -360 mV) and methyl viologen (-440 mV), and less effectively by triquat (-540 mV). The difference spectrum of Cyt c had negative peaks at 551, -520 and -420 nm, with a positive rise a -440 to -500 nm. The difference spectrum of CF_A/CF_B resembled P430 of PSI,and had a broad negative peak at 430-435 nm.
Abbreviations : (B) Chl- (bacterio) chlorophyll ; Cyt-cytochrome ; F_A, F_B and F_X-iron-sulfur center A,B and X of photosystem I ; CF_A, CF_B and CF_X-F_A-, F_B- and F_X-like Fe-S center of Chlorobium ; mPMS-1-methoxy-5-methylphenazinium methyl sulfate, PSI-Photosystem I ; RC-reaction center
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