| Project/Area Number |
06640861
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物形態・構造
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| Research Institution | Kanazawa University |
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Principal Investigator |
SASAYAMA Yuichi Faculty of Science, Kanazawa University, Professor, 理学部, 教授 (30018999)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Nobuo Faculty of Science, Kanazawa University, Assistant Professor, 理学部, 助手 (60242476)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Reptile hormone / Calcitonin primary structure / Morecular evolution / 爬虫類 / カルシトニン |
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| Research Abstract |
Amplifying of calcitonin gene of 4 species of reptiles (caiman : Gaiman crocodiles, snake : Elaphe climacophora, skink : Takydromus taky-dromoides and turtle : Geoclemys reevisii) was tried using genomic polymerase chain reaction (PCR) method. When primer designed from 2 parts of base sequence of chicken calcitonin gene was applied, about 150 bp of DNA fraction, of which sizu is similar to that of human calcitonin gene, was amplified in each species of those reptiles. After cloning them, it became clear that each base sequence was very similar to that of chicken calcitonin gene. The similarity was 100% in the turtle, 99% in the caiman, 96% in the skink and 93% in the snake. The order of the similarity was coincident to the fact that these species was differentiated from their ancestors in the same order (turtle is the oldest) in reptiles.
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