• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

MOLECULAR MECHANISM OF PLOIDY-SHIFT AND VARIATION OF COLONY MORPHOLOGY IN THE IMPERFECT YEASTS

Research Project

Project/Area Number 06640865
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物形態・構造
Research InstitutionNARA WOMEN'S UNIVERSITY

Principal Investigator

SUZUKI Takahito  NARA WOMEN'S UNIVERSITY,DPT.OF BIOLOGY FULL PROFESSOR, 理学部, 教授 (60144135)

Co-Investigator(Kenkyū-buntansha) IWAGUCHI Shinichi  NARA WOMEN'S UNIVERSITY,DPT.OF BIOLOGY ASSISTANT PROFESSOR, 理学部, 講師 (40263420)
Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsIMPERFECT YEAST / Candida albicans / PLOIDY-SHIFT / COLONY MORPHOLOGY / ZYGOTIC VARIATION / CHROMOSOME REARRANGEMENT / ELECTROPHORETIC KARYOTYPE / 電気泳動核法 / 集落形態 / カンジダ / 倍数性 / 接合性 / アデニン遺伝子 / 高次倍数性
Research Abstract

Some strains of Candida albicans poroduce a proportion of the cell population with a higher ploidy than the rest in normal diploid state. This phenotype was named as Pld^-by the author. Ploidy-shift was observed between two different ploidy states. Pld-STN21 (arg4, Pld^-) was isolated from a weakly growing sector of a half-sectored colony, after UV-irradiation of STN14, an arg4 derivative of C.albicans CBS5736 and from the other normally growing sector, STN22 (arg4, Pld^+) was isolated. STN22, as well as its progenitor, CBS5736, showed a homogeneus colony size when plated out, but STN21 showed a characteristic heterogeneity of colony size on YPD plates at an early stage of incubation : two types of colonies, small ones and large ones, were observed.
The chromosomes of these strains were separated out by PFGE.Some culture of STN21 showed the single band of chromosome 7, suggesting due to the occurrence of chromosome non-disjunction. The other culture of STN21 gave an extrachromosomal band, the size of which was larger than that of chromosome 7, and the loss of one of the two bands of chromosome 7. Southern blot hybridization experiments showed the existence of a SfiI fragment 7F,a central portion of chromosome 7, on the extrachromosomal band but the absence of a terminal SfiI fragment 7C.Protoplast fusion experiments showed, at least, two complementation groups of PLD,the function of which may stabilize both ploidy state and chromosome fidelity.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Sakaguchi,S.,Miyamoto,S.,Iida,H.,Anraku,Y.,Suzuki,T.& Ohya,Y.: "Overpnoduction of Cdc 24p (cls4p), a GTP-GDP enchange factor toward Cdc42 GTPase impairs initiation of budding in S.carevisiae" Protoplasma. 189. 142-148 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yano,S.,Inoue,S.,Noguchi,R.,Kato,M.,& Suzuki,T.: "Bioactivity of nickel (II) coxplex containing N-glycosides derived from D-glucosaxine and ethlenediamine diamune against posloenic yeast" Biological & Pharmaceutical Bullletin. 18. 923-925 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki,T.,Yamada,M.,& Sakaguchi,S.: "Occurrence of chromosone rearrangements during the fusion process in the imperfect yeast candida albicans." Microbiology. 140. 3319-3326 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ono,T.,Suzuki,T.,Anroku,Y.,& Iida,H.: "The MID2 gene encodes a putative integral membrane protein with a Ca^<++>-binding domain and shows mating pheromone-stimulated expression in S.cerevisiae." Gene. 151. 203-208 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzaki,T.,Hitomi,A.,Mogee.T.& Sakaguchi,S.: "Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans." Journal of Bacteriology. 176. 3345-3353 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鈴木孝仁: "真菌の二形性とその調節" Plant Morphology. 7. 29-38 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 1) Sakaguchi, S., Miyamoto, S., Iida, H., Anraku, Y., Suzuki, T., Ohya, Y., and Anraku, Y.: "Overproduction of Cdc24p (cls4p), a GTP-GDP exchange factor toward Cdc42 GTPase impairs initiation of budding in Saccharomyces cerevisiae." Protoplasma. 189. 142-148 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 2) Yano, S., Inoue, S., Nouchi, R., Kato, M., and Suzuki, T.: "Bioactivity of nickel (II) complex containing N-glycosides dirived from d-glucosamine and ethylenediamine against pathogenic yeast, Candida albicans." Biological & Pharmaceutical Bulletin. 18. 923-925 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 3) Suzuki, T., Yamada, M., and Sakaguchi, S.: "Occurrence of chromosome rearrangements during the fusionprocess in the imperfect yeast Candida albicans." Microbiology. 140. 3319-3328 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 4) Ono, T., Suzuki, T., Anraku, Y., and Iida, H.: "The MID2 gene encodes a putative integral membrane protein with a Ca2+-binding domain and shows mating pheromone-stimulated expression in Saccharomyces cerevisiae." Gene. 151. 203-208 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 5) Suzuki, T., Hitomi, A., Magee, P.T., and Sakaguchi, S.: "Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans." Journal of Bacteriology. 176. 3345-3353 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakaguchi,S.,Miyamoto,S.,Iida,H.,Anraku,Y.,Suzuki,T.& Ohya,Y.: "Overpnoduction of Cdc 24p(cls4p),a GTP-GDP ekchange factor toward Cdc42 GTPase impairs initiation of budding in S,cevevisiae" Protoplasma. 189. 142-148 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yano,S.,Inoue,S.,Noguchi,R.,Kato,M.,& Suzuki,T.: "Bioactivity of nickel(II)complex containing N-glycasides derived from D-glucosamine and ethylenediamine against postogenic yeast." Biological & Pharmaceutical Bulletin. 18. 923-925 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki,T.,Yamada,M.,& Saskaguchi,S.: "Occurrence of chromosome rearrangements during the fusion process in the imperfect yeast Candida albicans," Microbiology. 140. 3319-3326 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ono,T.,Suzuki,T.,Anraku,Y.,& Iida,H.: "The MID2 Gene ecodes a putative integral nembane protein with a Ca♯-binding domaina and shows nating pheromone-stimulated expression in S.cerevisiae." Gene. 151. 203-208 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki,T.,Hitomi,A.,Mogee,P.T.& Sakaguchi,S.: "Correlation between polyploidy and auxotrophic segregation in the imperfect yeast Candida albicans." Journal of Bacteriology. 176. 3345-3353 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木孝仁: "真菌の二形性とその調節" Plant Morphology. 7. 29-38 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki,T.et al: "Corvelation between polyploidy and auxotrophic segregation in the imperfect yeast candido albicans." Journal of Bacteriology. 176. 3345-3353 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ono,T.Suzuki,T.,Anraku,Y,and Iida,H.: "The MID2 gene encodes a putative integral membrane protein with a Ca^<2±>binding domain and shows mating pheromon-stimulated expression in S.cerevislae." Gene. 151. 203-208 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Suzuki,T.Yamada,M.and Sakaguchi,S.: "Occurrence of chromosome veorrongements during the fusion process in the imperfect yeast Candida albicans." Microbiology. 140. 3319-3328 (1994)

    • Related Report
      1994 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi