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MOLECULAR PHYLOGENETIC STUDY OF DROSOPHILA

Research Project

Project/Area Number 06640906
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionTOKYO METROPOLITAN UNIVERSITY

Principal Investigator

AOTSUKA Tadashi  Faculty of Science TOKYO METROPOLITAN UNIVERSITY Assistant Professor, 理学部, 助教授 (40106604)

Co-Investigator(Kenkyū-buntansha) TAMURA Koichiro  Faculty of Science TOKYO METROPOLITAN UNIVERSITY Assistant, 理学部, 助手 (00254144)
FUJITA Hiroshi  Faculty of Science TOKYO METROPOLITAN UNIVERSITY Assistant, 理学部, 助手 (20094279)
Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsDrosophila / Molecular Phylogeny / Adh / mtDNA / Hawaiian Drosophila / アルコール脱水素酵素 / Adh遺伝子
Research Abstract

On the basis of internal marphology and biogeography, Throckmorton (1966,1975) proposed a hypothesis for phylogenetic relationships between the species group of the family Droso-philidae, which has been widely accepted by many evolutionary biologists. On the other hand, using a caldistic analysis of many external morphological traits, Grimaldi (1990) recently put forward another hypothesis that was very different from Throckmorton's. We therefore re- examined phlogenetic relationships between the species of the family Drosophilidae by nucleotide sequences of the alcohol dehydrogenase (ADH) and the mitochondrial ND2 genes. The molecular phylogenetic trees obtained from these sequences were significantly different from Grimaldi's hypothesis. On the whole, our results supported the Throckmorton's hypothesis. However, our trees offered some new knowledege for understanding Drosophila phylogeny which have not been pointed out neither in Throckmorton's or Grimaldi's hypotheses. Among those, a information for the origin of Hawaiian drosophilids is very interesting. The hypothesis that Hawaiian drosophilids originated in the immigrans- Hirtodrosohila radiation (Throckmorton, 1975) has been widely accepted by many drosophilists. In this study, however, this group is found to be more closely related to the species of the virilis- repleta lineage that diverged from the immigrans- Hirtodrosohila lineage.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Watabe H.,J.Park and T.Aotsuka: "A Karyotype study on the Drosophila robusta species group." Zoological Science. 14巻(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura K.,G.Toba,J.Park and T.Aotsuka: "Origin of Hawaiian Drosophilids inferred from alcohol dehydrogenase gene sequences" Current Topics of Molecular Evolution. 9-18 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komiya,K.,T.Kondoh and T.Aotsuka: "Evolution of the noncoding regions in Drosophila mtDNA:Two intergenic regions" Biochemical Genetics. vol.33. 73-82 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aotsuka,T.,H.Y Chang,M,Aruga,F-J Lin and O.Kitagawa: "Mitochondrial DNA variation in natural populations of Drosophila immigrans" Zoological Studies. vol.33. 29-33 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kumar.S.,K.Tamura and M.Nei: "MEGA:Molecular evolutionary genetics analysis soft-ware for microcomputers" CABIOS. vol.10. 189-191 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohsako.T.,T.Aotsuka and O.Kitagawa: "The origin of the Japanese mainland population of Drosophila albomicans" Japanese J.Genetics. vol.69. 183-194 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aotsuka,T.,T.Suzuki,T.Moriya and A.Inaba: "Genetic differentiation in Japanese freshwater crab,Geothelphusa dehaani(white):Isozyme variation among natural populations in kanagawa Prefecture and Tokyo" Zoological Science. vol.12. 427-434 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura,K: "Model selection in the estimation of the number of nucleotide substitution" Molecular Biology and Evolution. vol.11. 154-157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watabe H., J.Park, and T.Aotsuka: "A karyotype study on the Drosophila robusta species group (Diptera : Drosophilidae)." Zool. Sci. (In press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura K., G.Toba, J.Park, and T.Aotsuka: "Origin of Hawaiian Drosophilids inferred from alcohol dehydrogenase gene sequences." Current Topics of Molecular Evolution. 9-18 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aotsuka, T., T.Suzuki, T.Moriya and A.Inaba: "Genetic differentiation in Japanese freshwater crab, Geothelpusa dehaani (White) : Isozyme variation among natural populations in Kanagawa Prefecture and Tokyo." Zool. Sci.12. 427-434 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komiya, K,T.Kondoh and T.Aotsuka: "Evolution of the noncoding regions in Drosophila mtDNA : Two intergenic regions." Biochem. Genet.33. 73-82 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Aotsuka, T., H- Y Chang, M.Aruga, F-J Lin and O.Kitagawa: "Mitochondrial DNA variation in naural populations of Drosophila immigrans." Zool. Sci.33. 29-33 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kumar, S., K.Tamura and M.Nei: "MEGA : Molecular evolutionary genetics analysis soft- ware for microcomputers." CABIOS. 10. 189-191 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tamura, K.: "Model selection in the estimation of the number of nucleotide substitutions." Mol. Biol. Evol.11. 154-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ohsako, T., T.Aotsuka and O.Kitagawa: "The origin of the Japanese mainland population of Drosophila albomicans." Japan. J.Genet.69. 183-194 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Watabe H- a.et al.: "A Karyotype study on the Drosophila robusta species- group (Diptera : Drosophilidae)" Zool.Sci.14(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Komiya.K.et al.: "Evolution of the noncoding regions in Drosophila mitochondrial DNA : Two intergenic regions." Biochemical.Genetics. 33. 73-82 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohsako,T.et al.: "The origins of the Japanese mainland population of Drosophila albomicans." Japanese.Journal.Genetics. 69. 183-194 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aotsuka,T.et al.: "Geographic variation in mitochondrial DNA of Drosophila immigrans." Zoological Studies. 31. 29-38 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kumar,S.et al.: "MEGA : Molecular Evolutionary Genetics Analysis software for microcomputers." CABIOS. 10. 189-191 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamura,K.: "Model selection in the estimation of the number of nucleotide substitutions." Mol.Biol.Evol.11. 154-157 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tamura,K.et al.: "Current Topics on Molecular Evolution" Origin of Hawaiian drosophilids inferred from alcohol dehydrogenase gene sequences., 9 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Aotsuka,T.: "Genetic differentiation in Japanese freshwater crab,Geothelphusa dehaani(white):Isozyme variation among natural populations in Kanagawa Pretecture and Tokyo" Zoological Science. 12. 427-434 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Komiya,K.: "Evolution of the noncoding regions in Drosophila mitochondrial DNA:Two intergenic regions." Biochemical Genetics. 33. 73-82 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ohsako,T.: "The origins of the Japanese mainland population of Drosophila albomicans." Japanese Journal of Genetics. 69. 183-194 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Aotsuka,T.: "Geographic variation in mitochondrial DNA of Drosophila immigrans." Zoological Studies. 31. 29-33 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tamura,K.: "Model selection in the estimation of the number of nucleotide substitutions." Molecular Biology and Evolution. 11. 154-157 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kumar,K.: "MEGA:Molecular Evolutionary Genetics Analysis software of microcomputers" CABIOS. 10. 189-191 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] K.Komiya, T.Kondoh, T.Aotsuka: "Evolution of the noncoding regions in Drosophila mitochondrial DNA: Two intergenic regions." Biochemical Genetics. 33(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Ohsako, T.Aotsuka, O.Kitagawa20GB02:The origins of the Japanese mainland population of Drosophila albomicans.: Japanese Journal of Genetics. 69. 183-194 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Aotsuka, M.Aruga, H.Y.Chang, F.J.Lin, O.Kitagawa: "Geographic veriation in mitochondrial DNA of Drosophila immigrans." Zoological Studies. 31. 29-33 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Tamura: "Model selection in the estimation of the number of nucleotide substitutions" Molewlar Biology and Evolution. 11. 154-157 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Kumar, K.Tamura: "MEGA: Molewlar Evolutionary Genetics Analysis sattware for microcomputers" CABIOS. 10. 189-191 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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