| Project/Area Number |
06650914
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka University |
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Principal Investigator |
KUBOI Ryoichi Osaka University, Department of Chemical Engineering, Associate Professor, 基礎工学部, 助教授 (40029567)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOMORI Koichiro Miyazaki University Dept.of Material Engineering, Research Fellow, 工学部, 助手 (80235506)
KOMASAWA Isao Osaka University, Dept.of Chemical Engineering, Professor, 基礎工学部, 教授 (40029476)
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| Project Period (FY) |
1994 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1995: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Riverse micelle / Mixed micells / Lecithin / AOT / Partition chromatograph / CPC / Bioreactor / beta-Galactosidase / 遠心溶液分配クロマト |
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| Research Abstract |
The application of reverse micellar micro bioreactor systems for enzymatic conversion with continuous recovery of water-soluble products was demonstrated, based on the production of galactose by micelles containing beta-galactosidase. The beta-gal was firmly encapsulated in AOT (bis (2-ethylhexyl) sodium sulfosuccinate) /lecithin mixed micelles. The addition of lecithin to the AOT reverse micelles stabilized the micellar interface, and the leakage of the encapsulated beta-gal to the bulk aqueous phase was therefore negligible, while substrate and product were free to transfer and partition owing to their low molecular weights. The stability of the solubilized beta-gal was also increased in the AOT/lecithin mixed micelles as compared to the conventional AOT micelles. A consider ably higher activity was observed in the present systems than in the bulk buffer solution. Enzymatic conversion and product recovery was carried out in a continuous operation mode by use of centrifugal partition chromatography (CPC), employing the reverse micellar solution with encapsulated beta-gal as the stationary phase and the substrate aqueous solution as the mobile phase. Use of the CPC contactor eliminated any mass transfer limitation on the substrate and the product, and galactose was continuously recovered in the effluent.
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