• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the development of allergy treatment using gene-knockout mice.

Research Project

Project/Area Number 06650918
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 生物・生体工学
Research InstitutionOkayama University

Principal Investigator

OHMORI Hitoshi  Professor Department of Biotechnology, Faculty of Engineering, Okayama University, 工学部, 教授 (70116440)

Project Period (FY) 1994 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1995: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsgene targeting / Fc gamma receptor / gene-knockout mouse / allergy / anaphylaxy / IgG immune complex / B cells / mast cells / Fcガンマレセプター / 抗体 / 遺伝子ノックアウト / ES細胞 / 変異マウス / IgE抗体 / リンパ球 / IgG Fc受容体 / クラススイッチ / 遺伝子ターゲッティング / ノックアウトマウス
Research Abstract

The present research project is unique in that we attempted to develop technologies for the treatment of allergic diseases by focusing the role of Fc gamma receptor II (FcgammaRII). For this purpose, we established FcgammaRII-deficient mice by gene-targeting as follows. Targeting vector. A.2.65-kb BamHI fragment containing S1 and EC1 exon was replaced by a neo-resistant gene cassette. Homologous J1 ES cell recombinants were obtained at a frequency of 10.4%. Offsprings of heterozygous intercrosses were examined for the gene knockout by Southern blot of genomic DNA isolated from tail tips. It was found that one out of four offsprings possessed the homozygous disrupted gene in accordance with Mendelian frequency. Lack of FcgammaRII protein was confirmed on B cells, macrophages and mast cells by flow cytometry. Sensitivities of this animal to various allergic stimuli are being investigated.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] T, Takai, H. Ohmori et al.: "Augmented humoral and anaphylactic responses in FcγRII-deficient mice." Nature. 378. 346-349 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] N, Hase, H, Ohmori et al.: "Predominant suppression of anti TNP igE response in mice by monoclonal anti-TNP IgG1 antibody" Int. J. Immunopharmacol.16. 787-794 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toshiyuki Takai, Masao Ono, Masaki Hikida, Hitoshi Ohmori and Jeffery Ravetch: "Augmented humoral and anaphylactic responses in FcgammaRII-deficient mice." Nature. Vol.378. 346-349 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Naoki Hase, Toshiyuki Takai, Masaki Hikida and Hitoshi Ohmori: "Predominant suppression of anti-TNP IgE response in mice by monoclonal anti-TNP IgG1 antibody. Characterization of its mode of action by in vivo and in vitro studies." Int.J.Immunopharmacol.Vol.16. 787-794 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Takai,H.Ohmori et al.: "Augmented humoral and anaphylactic responses in FcγR II-deficient mice." Nature. 379. 346-349 (1996)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1994-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi