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Comparative Study on Species-Specific Seed Constituents Differenciating between Wild and Cultivated Soybeans

Research Project

Project/Area Number 06660004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionIwate University

Principal Investigator

KAIZUMA Norihiko  Iwate University, President, 教授 (00003773)

Project Period (FY) 1994 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsSoybeans / Wild Soybeans / Trypsin-Inhibitor / Seed Proteins / Ecotype Differenciation / Gene Resources / 種子タンパク質
Research Abstract

The chemical seed constituents differentiating between cultivated and wild soybeans were not detected. However, partitional ratios of the constituents among leaves, stems and seeds seemed to differ specifically between the two species.
Estimation of the north and south limits in growing area of wild soybean in Japan was determined as in the southern Hokkaido and Kyushu by field investigations.
Principal Component Analysis using the 30 morphological traits revealed that at least the two ecotypes exisi in Japan, Southern and Northern.
Some new electrophoretic polymorphic types of Kunitz trypsin inhibitor in seed were clearly detected. The DNA sequence of a new type named Tia-s was analyzed. As a result, an insertion and a substitution of the DNA bases of the gene and their respective Amino Acid changes were found.

Report

(4 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • 1994 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Wang,J.K.,et al.: "Detection of two new variants of soybean Kunitz trypsin inhibitor through electrophoresis" Breeding Science. 46. 39-44 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wang,J.K.,et al.: "The mode of inheritance and the DNA sequence analysis for a new soybean Kunitz type trypsin inhibitor vaariant, Tia-s" Breeding Science. 46,Suppl.2,. 89-89 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wang, J.K., et al.: "Detection of two new variants of soybean Kunitz trypsin inhibitor through electrophoresis" Breeding Science. 46. 39-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wang, J.K., et al.: "The mode of inheritance and the DNA sequence analysis for a new soybean Kunitz type trypsin inhibitor vaariant, Tia-s" Breeding Science. 46, Suppl.2. 89-89 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 王克晶ら: "ダイズKunitz型トリプシン・インヒビターTia-s変異体の遺伝分析と塩基配列決定" 育種学雑誌. 46別2. 244 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 王 克晶ら: "ダイズトリプシン・インヒビターの新しい電気泳動的多型" 育種学雑誌. 43(別2). 177-177 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wang.J.K.,et al.: "Detection of two new variants of soybean Kunitz trypsin inhibitor through electrophoresis." Breed.Sci.46. 39-44 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 王 克晶: "ダイズトリプシン・インヒビターの新しい電気泳動的多型" 育種学雑誌. 43(別2). 177〜177 (1993)

    • Related Report
      1994 Annual Research Report

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Published: 1994-04-01   Modified: 2016-04-21  

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